Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line

Lyu, Qingkang; Wawrzyniuk, Magdalena; Rutten, Victor P.M.G.; Eden, Willem van; Sijts, Alice J.A.M.; Broere, Femke

doi:10.3390/ijms21186464

Cited by 29 publications

(21 citation statements)

References 56 publications

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“…NF-κB has been reported to play a central role in the antioxidant and anti-inflammatory responses, and also is considered as a critical transcription factor required for the expression of many cytokines [ 22 ]. Therefore, the expression of NF-κB was examined by western blot assay.…”

Section: Resultsmentioning

confidence: 99%

ROCK inhibitor fasudil reduces the expression of inflammatory factors in LPS-induced rat pulmonary microvascular endothelial cells via ROS/NF-κB pathway

Liu

Pan

et al. 2022

BMC Pharmacol Toxicol

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Background Inflammation plays a major role in the pulmonary artery hypertension (PAH) and the acute lung injury (ALI) diseases. The common feature of these complications is the dysfunction of pulmonary microvascular endothelial cells (PMVECs). Fasudil, the only Rho kinase (ROCK) inhibitor used in clinic, has been proved to be the most promising new drug for the treatment of PAH, with some anti-inflammatory activity. Therefore, in the present study, the effect of fasudil on lipopolysaccharide (LPS)-induced inflammatory injury in rat PMVECs was investigated. Methods LPS was used to make inflammatory injury model of rat PMVECs. Thereafter, the mRNA and protein expression of pro-inflammatory factors was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) assay respectively. Intracellular reactive oxygen species (ROS) levels were measured by the confocal laser scanning system. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) were determined by using commercial kits according to the manufacturer’s instructions. Western blot assay was used to detect the protein expression of nuclear factor kappa B (NF-κB) p65. Results Fasudil effectively prevented inflammatory injury induced by LPS, which is manifested by the decrease of pro-inflammatory cytokines interleukin-6 (IL-6) and monocyte chenotactic protein-1 (MCP-1). Meanwhile, fasudil dramatically reduced the levels of ROS and MDA, and also elevated the activities of SOD and GSH-Px. Furthermore, the nuclear translocation of NF-κB p65 induced by LPS was also suppressed by fasudil. Additionally, the ROS scavengers N-Acetylcysteine (N-Ace) was also found to inhibit the nuclear translocation of NF-κB and the mRNA expression of IL-6 and MCP-1 induced by LPS, which suggested that ROS was essential for the nuclear translocation of NF-κB. Conclusions The present study revealed that fasudil reduced the expression of inflammatory factors, alleviated the inflammatory and oxidative damage induced by LPS in rat PMVECs via ROS-NF-κB signaling pathway.

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Section: Resultsmentioning

confidence: 99%

ROCK inhibitor fasudil reduces the expression of inflammatory factors in LPS-induced rat pulmonary microvascular endothelial cells via ROS/NF-κB pathway

Liu

Pan

et al. 2022

BMC Pharmacol Toxicol

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“…Recently, TLR‐mediated autophagy is reported to promote immune responses via increasing antigen processing and presentation in antigen‐presenting cells (Chou et al, 2020). Lyu et al (2020) research has shown that the upregulation of HSP70 plays a critical role in modulating LPS‐induced NF‐κB activation and cytokine expression. The increase of HSP70 in muscle and spinal cord, which may activate TLR4 in spinal cord microglia with consequent activation of an intracellular signaling cascade mediated by MyD88, resulting in NF‐қB activation and the production of proinflammatory cytokines, such as TNF‐α and IL‐6 (Dos Santos et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…ARF6 interacts with Rab proteins to regulate phagolysosome formation by regulating GTPase activity in P. vannamei gills cells. In hepatopancreas tissue, ARF6 plays a role in immunity of V. anguillarum infection by interacting with cytohesin.Recently, TLR-mediated autophagy is reported to promote immune responses via increasing antigen processing and presentation in antigen-presenting cells(Chou et al, 2020) Lyu et al (2020). research has shown that the upregulation of HSP70 plays a critical role in modulating LPS-induced NF-κB activation and cytokine expression.…”

mentioning

confidence: 99%

Transcriptome analysis reveals immune‐related differentially expressed genes in the hepatopancreas and gills of Penaeus vannamei after Vibrio anguillarum infection

Song

Soomro

et al. 2021

Aquaculture Research

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Penaeus vannamei is one of the commercially important aquaculture species in the world but sensitive to Vibrio anguillarum infection. To understand the immune response of P. vannamei against V. anguillarum infection, the transcriptomes of hepatopancreas and gills of P. vannamei were analysed before and after V. anguillarum infection. Total 6213 differentially expressed genes (DEGs) were obtained from the hepatopancreas, and 3780 were obtained from the gills. The DEGs of interest were screened to construct a heat map. The reliability and accuracy of the transcriptome data were verified using RT‐qPCR. HSP70 and ARF6 were found highly expressed in both hepatopancreas and gills, while MLC2s encoded by two different gene sequences were found to be expressed in hepatopancreas and gills. Six immune‐related DEGs were identified from the protein–protein interaction (PPI) network including the HSP70, ARF6 and MLC2s. The sub‐network of PPI was constructed to explore the mode of interaction of immune‐related DEGs with their neighbouring proteins. Based on the result of PPI network, we infer that phosphorylated MLC2‐1 conducts signal transduction to induce leukocyte transendothelial migration in P. vannamei gills tissue while MLC2‐2 plays a role in immunity of V. anguillarum infection not only through activating signal transduction to mediate immune responses but also through the formation of phagosomes in hepatopancreas tissue. HSP70 is associated with the transporting exogenous cargos through presenting antigenic peptides in the process of V. anguillarum infection in P. vannamei. ARF6 interacts with Rab proteins to regulate phagolysosome formation by regulating GTPase activity in P. vannamei gills tissue. In hepatopancreas tissue, ARF6 plays a role in immunity by interacting with cytohesin. This study will contribute to further exploring the immune mechanism of P. vannamei to V. anguillarum infection.

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“…HSPs can prevent or arrest inflammatory damage, in which they enhance the production of anti-inflammatory cytokines (Borges et al 2012). The anti-inflammatory effect of HSP may be due to their inhibitory action of signaling pathways NF-κB and NF-κB/nuclear factor kappa B (IκB), possibly through the prevention of IκB kinase activation that stabilizes IkB (Janus et al 2011;Lyu et al 2020). Induced Hsp70 inhibited the production of pro-inflammatory cytokines, such as TNFα, IL1β, and IL6 in vitro (Wang et al 2017).…”

Section: Transcriptomic Profile Changes During Oxidative Stressmentioning

confidence: 99%

Stress and immunity in poultry: light management and nanotechnology as effective immune enhancers to fight stress

Abo‐Al‐Ela

El‐Kassas

El-Naggar

et al. 2021

Cell Stress and Chaperones

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The poultry industry plays a significant role in boosting the economy of several countries, particularly developing countries, and acts as a good, cheap, and affordable source of animal protein. A stress-free environment is the main target in poultry production. There are several stressors, such as cold stress, heat stress, high stocking density, and diseases that can affect birds and cause several deleterious changes. Stress reduces feed intake and growth, as well as impairs immune response and function, resulting in high disease susceptibility. These effects are correlated with higher corticosteroid levels that modulate several immune pathways such as cytokine-cytokine receptor interaction and Toll-like receptor signaling along with induction of excessive production of reactive oxygen species (ROS) and thus oxidative stress. Several approaches have been considered to boost bird immunity to overcome stress-associated effects. Of these, dietary supplementation of certain nutrients and management modifications, such as light management, are commonly considered. Dietary supplementations improve bird immunity by improving the development of lymphoid tissues and triggering beneficial immune modulators and responses. Since nano-minerals have higher bioavailability compared to inorganic or organic forms, they are highly recommended to be included in the bird's diet during stress. Additionally, light management is considered a cheap and safe approach to control stress. Changing light from continuous to intermittent and using monochromatic light instead of the normal light improve bird performance and health. Such changes in light management are associated with a reduction of ROS production and increased antioxidant production. In this review, we discuss the impact of stress on the immune system of birds and the transcriptome of oxidative stress and immune-related genes, in addition, how nano-minerals supplementations and light system modulate or mitigate stress-associated effects.

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Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line

Cited by 29 publications

References 56 publications

ROCK inhibitor fasudil reduces the expression of inflammatory factors in LPS-induced rat pulmonary microvascular endothelial cells via ROS/NF-κB pathway

ROCK inhibitor fasudil reduces the expression of inflammatory factors in LPS-induced rat pulmonary microvascular endothelial cells via ROS/NF-κB pathway

Transcriptome analysis reveals immune‐related differentially expressed genes in the hepatopancreas and gills of Penaeus vannamei after Vibrio anguillarum infection

Stress and immunity in poultry: light management and nanotechnology as effective immune enhancers to fight stress

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