hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis for species identification and differentiation of Mycobacterium kansasii and Mycobacterium chelonae–Mycobacterium abscessus group

Sajduda, Anna; Martin, Anandi; Portaels, Françoise; Palomino, Juan Carlos

doi:10.1016/j.ijid.2011.11.011

Cited by 10 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems that; in order to decontamination, more concentration of CPC is suitable, in NTM isolation from surface water samples. Water has been proven as important source for these opportunistic mycobacteria [15,16] [17]. In the present study, of 62 water specimens collected from different sources, 4 were positive for M. fortuitum in culture.…”

Section: Discussionmentioning

confidence: 55%

Species distribution of Non tuberculosis mycobacteria isolates from North of Iran

Feizabadi¹,

Falsafi²,

Khavari-Nejad³

et al. 2014

The Journal of Genes, Microbes and Immunity

View full text Add to dashboard Cite

Nontuberculous mycobacteria (NTM) differ from tuberculous mycobacteria as most of them are ubiquitous and saprophytic. A few are considered to have the potential to infect humans. The immunological status of a person determines the advance of disease. On the other hand among environmental factors, water plays the main roles as a resource of contamination in transferring this group of microorganisms to human. The aim of this study is to identify and isolate NTM in the different region of Iran surface water. 62 water samples were collected from different regions of Northern Iran. Cetyl pyridinium chloride (cpc) 0.01% was used to decontaminate the water samples. After enrichment using filtration, all samples were incubated on Lowenstein-Jensen (LJ) medium at temperatures of 30 and 37 °C. Classical culture, biochemical and enzymatic methods are described for the identification of mycobacteria. hsp65 gene has more variability in its sequences, compared to the some more conserved genes in NTM, for identification of mycobacteria to species level. A 441 bp fragment of hsp65 genes was amplified and digested by two restriction enzymes, BstEII and HaeIII. Digested products were analyzed using 3% agarose gel electrophoresis. from the total of 62 water samples, 8(50%) mycobacteria have been isolated. There were different hsp65 gene PCR-RFLP profiles produced by digestion with BstEII and HaeIII. Dominant isolates were M. fortuitum. Other isolates of Mycobacteria were differentiated using hsp65 genes PCR-RFLP. This study indicates that notable percentage of water sources are contaminated with mycobacterium species. The exposure of immunocompromised individuals to these sources can have crucial consequences. Interestingly the prevalence of mycobacterium species differs in diverse geographical areas.

show abstract

Section: Discussionmentioning

confidence: 55%

Species distribution of Non tuberculosis mycobacteria isolates from North of Iran

Feizabadi¹,

Falsafi²,

Khavari-Nejad³

et al. 2014

The Journal of Genes, Microbes and Immunity

View full text Add to dashboard Cite

show abstract

“…hsp65 PRA currently has been adopted widely for identification of Nontuberculous mycobacteria (NTM) which was the method used in this case. This system involves coupling of PCR of a heat shock protein and is followed by species-specific restriction fragment length identification 21 . M. chelona e are mostly sensitive to macrolide and aminoglycosides; however, routine susceptibility testing should be performed with various antibiotics 22.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium ChelonaeDeveloping Multidrug Resistance

Mannelli

Manoj²,

Nemakayala

et al. 2018

BMJ Case Reports

View full text Add to dashboard Cite

is a rapidly growing mycobacterium which is known to respond well to standard antibiotic treatment regimen. There are no specific guidelines for treatment. Antibiotics are chosen based on the bacterial sensitivity. Here we present a 47-year-old man with hip replacement who developed bright red papular generalised skin lesions and bilateral hip abscess. On workup, it was confirmed that was the causative organism. He was given 8 weeks of antibiotics; however, there was worsening of the hip abscess on interval imaging. The progression was most likely due to developing antibiotic resistance. Physicians should be aware of the rising resistance of this organism, and guide antibiotic therapy based on bacterial sensitivity to yield better outcomes.

show abstract

“…All these species form the M. chelonae-abscessus complex, which is the 3 rd most common pathogenic mycobacterial complex apart from the M. tuberculosis and M. avium complexes because of their similar phenotypes. DNA sequencing (e.g., hsp65, rpoB and secA) or PCR restriction enzyme analysis on a population of isolates enabled us to identify them (17)(18)(19)(20). M. abscessus is part of an RG-NTM group that includes at least 3 subtypes, namely, M. abscessus, M. massiliense, and M. bolletii.…”

Section: Discussionmentioning

confidence: 99%

“…The subtypes of M. abscessus share 99% sequence identity with one another, and they cannot be clearly discriminated by phenotypic or molecular tests (21). Over the past few decades, outbreaks and isolated cases of skin and soft tissue infections caused by M. chelonae and M. abscessus have been described in association with cosmetic surgery and tattooing (17)(18)(19)(20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

“…Some subspecies of M. abscessus demonstrate at least intermediate susceptibility to linezolid and imipenem. M. chelonae shows excellent in vitro susceptibility to tobramycin, clarithromycin, linezolid, amikacin, and tigecycline, but it is uniformly resistant to cefoxitin (16)(17)(18)(19)(20)(21)(22). Treatment for M. chelonae is generally more successful than that for M. abscessus.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cutaneous Infections Caused by Rapidly Growing Mycobacteria: Case Reports and Review of Clinical and Laboratory Aspects

Zhang¹,

Jiang

et al. 2015

Acta Derm Venerol

View full text Add to dashboard Cite

Skin and soft tissue infections caused by rapidly growing non-tuberculous mycobacteria (RG-NTM) have become a growing clinical concern over the past decades. These RG-NTM are ubiquitous environmental organisms and most are resistant to traditional antituberculous agents. In this report, we describe 3 cutaneous infections caused by RG-NTM, namely, Mycobacterium abscessus, M. chelonae, and M. conceptionense, and present the clinical and laboratory characteristics of these infections.

show abstract

hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis for species identification and differentiation of Mycobacterium kansasii and Mycobacterium chelonae–Mycobacterium abscessus group

Abstract: PRA based on automated capillary electrophoresis is a rapid, simple, and reliable method for the identification and differentiation of both clinically relevant and environmental isolates of M. kansasii and M. chelonae-M. abscessus group.

Cited by 10 publications

References 28 publications

Species distribution of Non tuberculosis mycobacteria isolates from North of Iran

Species distribution of Non tuberculosis mycobacteria isolates from North of Iran

Mycobacterium ChelonaeDeveloping Multidrug Resistance

Cutaneous Infections Caused by Rapidly Growing Mycobacteria: Case Reports and Review of Clinical and Laboratory Aspects

Contact Info

Product

Resources

About