Hsp27 and Hsp70 in chronic obstructive pulmonary disease: certainties vs doubts

Cappello, Francesco; Macario, Alberto J. L.; Stefano, Antonino Di

doi:10.1007/s12192-015-0617-9

Cited by 4 publications

(5 citation statements)

References 6 publications

(8 reference statements)

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“…2 However, the role of HSPs in chronic obstructive pulmonary disease (COPD) pathogenesis is still poorly understood and debated. 3 In the past, we showed in vivo that the levels of HSP60 decrease during the carcinogenic steps of bronchial mucosa in patients affected by COPD and, noteworthy, the normal epithelium close to the tumor shows a reduced immunopositivity for HSP60 compared to normal epithelium of healthy subjects as determined in mucosal biopsies. 4 In contrast, the intracellular HSP60 levels are increased in the bronchial epithelium of patients without cancer but with severe/very severe COPD compared to smoker and non-smoking subjects.…”

Section: Introductionmentioning

confidence: 96%

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HSP60 activity on human bronchial epithelial cells

Sangiorgi

Vallese

Gnemmi

et al. 2017

Int J Immunopathol Pharmacol

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HSP60 has been implicated in chronic inflammatory disease pathogenesis, including chronic obstructive pulmonary disease (COPD), but the mechanisms by which this chaperonin would act are poorly understood. A number of studies suggest a role for extracellular HSP60, since it can be secreted from cells and bind Toll-like receptors; however, the effects of this stimulation have never been extensively studied. We investigated the effects (pro- or anti-inflammatory) of HSP60 in human bronchial epithelial cells (16-HBE) alone and in comparison with oxidative, inflammatory, or bacterial challenges. 16-HBE cells were cultured for 1–4 h in the absence or presence of HSP60, H2O2, lipopolysaccharide (LPS), or cytomix. The cell response was evaluated by measuring the expression of IL-8 and IL-10, respectively, pro- and anti-inflammatory cytokines involved in COPD pathogenesis, as well as of pertinent TLR-4 pathway mediators. Stimulation with HSP60 up-regulated IL-8 at mRNA and protein levels and down-regulated IL-10 mRNA and protein. Likewise, CREB1 mRNA was up-regulated. H2O2 and LPS up-regulated IL-8. Experiments with an inhibitor for p38 showed that this mitogen-activated protein kinase could be involved in the HSP60-mediated pro-inflammatory effects. HSP60 showed pro-inflammatory properties in bronchial epithelial cells mediated by activation of TLR-4-related molecules. The results should prompt further studies on more complex ex-vivo or in-vivo models with the aim to elucidate further the role of those molecules in the pathogenesis of COPD.

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Section: Introductionmentioning

confidence: 96%

“… 2 However, the role of HSPs in chronic obstructive pulmonary disease (COPD) pathogenesis is still poorly understood and debated. 3 …”

Section: Introductionmentioning

confidence: 99%

HSP60 activity on human bronchial epithelial cells

Sangiorgi

Vallese

Gnemmi

et al. 2017

Int J Immunopathol Pharmacol

Self Cite

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“…We have read with great interest the work by Cui et al (2015) and the letter to the Editor by Cappello et al (2015). Historical knowledge of data acquisition and interpretation is paramount in the field of applied medical research.…”

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confidence: 99%

“…By interpreting the report by Cui et al (2015) and its comment by Cappello et al (2015), we want to allude to a recent publication by our research group (Zimmermann et al 2014). It has to be accepted by the community that an ELISA detection kit for protein XY is sometimes not detecting protein XY (Mueller et al 2012).…”

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confidence: 99%

Serendipity and technical considerations for the measurement of serum heat shock protein HSP27 in patients with COPD and lung cancer

Ankersmit

Lambers

Zimmermann

et al. 2015

Cell Stress and Chaperones

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“…Dear Editor, We would like to thank the journal for giving us the opportunity to respond to a Letter to the Editor written by Cappello et al (2015) concerning our recent study (Cui et al 2015). We also thank Dr. Cappello and his colleagues for raising these important issues concerning the plasma levels of Hsp27 in people with chronic obstructive pulmonary disease (COPD).…”

mentioning

confidence: 99%