HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Tarkhan, Amneh H.; Saleh, Khaled Musa Mohammad; Al‐Zghoul, Mohammad Borhan

doi:10.3390/vetsci7020049

Cited by 12 publications

(9 citation statements)

References 56 publications

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“…Intermittent manipulation in incubation temperature between different embryonic ages resulted in thermoregulatory functions being boosted; 3 h of 39.5°C/d from E16 to E18 [21], 39.5°C for 12 h/d at E7-E16 [15,16,24], and 60 minutes exposure to 15°C at E18-E19 [57]. In our review, the similar effect was confirmed by [49,50] at 38.5-39.5°C for 18 h/d from E10 to E18 and, David et al [18] at 39.5°C for 18 h/d from E7 to E16. It's clear that thermotolerance acquisition in broiler chickens can be enhanced by application of TM between E7 to E18, a period that is termed the critical stage and, the ideal embryonic age for TM.…”

Section: Thermal Manipulation and Thermotolerance Acquistionsupporting

confidence: 72%

“…Among the 24 intermittent TM studies, 20 articles reported the TM effect on hatchability, of which 65% found no significant effect, 30% being reduced, and a comparative study by Dalab and Ali [46] reported increased and decreased hatchability at different embryonic age. Meanwhile, embryo and/or hatch weight and chick quality were reported in 18 articles, of which 12 (67%) found no significant effect, three studies reported increased [2,38,50], and three reported reduced effect of TM on embryo and/or hatch weight and chick quality [42,45,46].…”

Section: Search Strategy and Selection Of Publicationsmentioning

confidence: 99%

“…Hatch or chick body temperature was reported in eight of 24 intermittent TM studies, which included no significant effect [48,52], increased [30,42,51], and reduced [18,49,50].…”

Section: General Characteristics Of the Studies Included In The Syste...mentioning

confidence: 99%

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Thermal Manipulation: Embryonic Development, Hatchability, and Hatching Quality of Broiler Chicks

Tainika¹

2022

Broiler Industry

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Here, PRISMA guidelines were utilized to systematically evaluate the publications reporting the effect of thermal manipulation during embryogenesis on incubation performance, hatchability, and hatching quality of broiler chicks. The search and selection of eligible publications was through databases web of science, PubMed, and Scopus. Publications written in English between 2015 and September 2021 were considered. It is evidenced that during TM, key considerations include duration and strength of TM besides stage of embryonic development. The moderate elevation in incubation temperature (38.5–39.5°C) intermittently (3–18 h/d) between E07 and E18 improves the chick’s thermoregulation capacity and reduces any adverse effect of TM on hatchability, and chick quality (e.g., hatch weight and chick length) compared with continuous TM. In addition, high temperature TM (38.5–39.5°C) between E7 and E18 has no significant effect on embryo mortality, hatchability, and chick quality compared to standard incubation temperature (37.8°C). TM above 39.5°C significantly increases and decreases embryo mortality and hatchability, respectively compared with standard incubation temperature. In conclusion, the results of TM studies on embryogenesis, hatchability and hatching quality of broiler chicks are still contradicting, which is a possible limitation for its commercial use.

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Section: Thermal Manipulation and Thermotolerance Acquistionsupporting

confidence: 72%

Section: Search Strategy and Selection Of Publicationsmentioning

confidence: 99%

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Thermal Manipulation: Embryonic Development, Hatchability, and Hatching Quality of Broiler Chicks

Tainika¹

2022

Broiler Industry

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“…Similarly, it was shown that chicks subjected to TM resulted had decreased HSF1 , HSF3 , and HSP70 expression in the jejunal mucosa after 7 days of HS exposure in comparison with chicks incubated under standard conditions [ 40 ]. Furthermore, CS was reported to induce the expression of HSF3 and HSP70 in the liver and spleen of broiler chickens after 5 days of exposure to CS, but TM mitigated this response [ 84 ]. During thermal stress, the decreased expression of heat shock proteins in TM chicks compared to controls suggests that TM chicks were less susceptible to thermal stress.…”

Section: Discussionmentioning

confidence: 99%

Molecular and morphometric changes in the small intestine during hot and cold exposure in thermally manipulated broiler chickens

2021

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Background and Aim: Thermal stress (hot or cold) is one of many environmental stressors that severely affects the health of broiler chickens. One negative effect of thermal stress is the disruption of the intestinal barrier function in broiler chickens. This study aimed to evaluate the effect of thermal manipulation (TM) on the small intestine in terms of histomorphometry as well as junctional, heat-shock, and immune response gene expression during post-hatch exposure to thermal stress. Materials and Methods: The experiment was conducted by dividing 928 fertile Ross eggs into three incubation groups: The control (C) group (incubated at 37.8°C and 56% relative humidity [RH] for the whole incubation period), the TM using low temperature TML group (incubated at 36°C and 56% RH for 18 h/day from embryonic days 7 to 16), and the TM using high temperature (TMH) group (incubated at 39°C and 65% RH for 18 h/day from embryonic days 7 to 16). On post-hatch day 21, 90 chicks were randomly selected from each incubation group and were equally subdivided into three subgroups for the post-hatch thermal stress experiment: The TN subgroup (room temperature maintained at 24°C), the heat stress (HS) subgroup (room temperature maintained at 35°C), and the cold stress (CS) subgroup (room temperature maintained at 16°C). After 1 day of thermal stress exposure (age 22 days), five birds from each subgroup were euthanized and ileum samples were collected to evaluate the transcription of the Claudin (CLDN1), CLDN-5, Occludin, Cadherin-1, heat shock factors (HSF1), HSF3, 70 kilodalton heat shock protein, 90 kilodalton heat shock protein, Interleukin 6 (IL6), IL8, toll-like receptors-2 (TLR2), and TLR4 genes by Real-Time Quantitative Reverse Transcription polymerase chain reaction analysis. Finally, after 4 and 7 days of thermal stress (age 25 and 28 days, respectively), nine chicks were euthanized, and their jejunum and ileum were collected for histomorphometric analysis. Results: After exposure to 1 day of thermal stress, the C subgroups exposed to thermal stress (HS and CS) possessed significantly increased expression of junctional, heat-shock, and immune response genes compared to the C-TN subgroup, and similar results were observed for the TMH. In contrast, thermally stressed TMH subgroups had significantly lower expression of the studied genes compared to C subgroups exposed to thermal stress. Furthermore, no significant changes were detected between the TML subgroups exposed to thermal stress and TML-TN. Moreover, significant alterations in villus height (VH), villus surface area, crypt depth (CD), and VH to CD ratio were observed between the TML, TMH, and C subgroups exposed to CS. Conclusion: It might be suggested that TM may have a protective impact on the small intestine histomorphometry and epithelial integrity of broilers during post-hatch exposure to thermal stress.

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“…Zhang and his colleagues [11] found that the level of HSP70 declined in the heart 6 hours after the heat stress, but the HSF3 expression remained high. Tarkhan and his colleagues [49] examined the HSP70 and HSF3 expression levels in cold stress. They found decreased expression levels in the liver in case of both genes.…”

Section: Discussionmentioning

confidence: 99%

Examination the effect of post-hatch heat-treatment and heat-stress in Transylvanian Naked Neck chicken

Tóth

Szabadi

Lázár

et al. 2020

Preprint

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Background One of the most critical global problem nowadays is the increased environmental temperature. Agriculture is very susceptible to this adverse effect because the productivity of animals and poultry decreased. Although several studies reported the effects of heat-stress in chicken, the expression profile of heat-shock proteins and heat shock factors was not investigated in the gonads and germ cells of Transylvanian Naked Neck chickens. Methods In the first experiment, 24 hours after hatching 80 chicks were heat treated on 38.5oC ambient temperature with 60% humidity for 12 hours. After maturation, their primary productivity parameters, such as egg production, abnormalities in embryo development, sperm quantity, concentration, and motility were studied following two weeks of heat-stress on 30 °C room temperature. In the second experiment, the thermal manipulation of 60 chicks was the same but 15 treated and 15 control chicks were sacrificed immediately after the treatment. The other 15–15 chickens were raised to maturity. Expression levels for two heat-shock proteins and four heat shock factors were determined by real-time PCR in the gonads of heat-treated and heat-stressed chickens. Results We found that the heat-treated layers had significantly higher egg production than the control group in heat-stressed conditions. In cockerels, the sperm quality did not differ significantly between the heat-treated and heat-stressed group and the heat-stressed but not heat-treated group. We examined the expression pattern of HSPs and HSFs in the gonads. We found that the expression of HSP90 and HSF4 increased significantly (p < 0.05) in heat-treated female chick gonads but in adult females the expression of HSF2 and HSF3 were significantly lower compared to the control. In case of adult heat-treated males, the HSP70, HSF1 and HSF3 expression levels showed a significant increase in both gonads, compared to the control expression levels (P < 0.05). Conclusion Heat shock proteins and heat-shock factors protect cells against different stressors, including heat stress. Our findings show a significant effect on egg production but not on the sperm quality after post-hatch heat treatment in heat stress condition. The presented significant differences might be related to the increased expression level of HSP90 and HSF4 in heat-treated chickens.

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HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Cited by 12 publications

References 56 publications

Thermal Manipulation: Embryonic Development, Hatchability, and Hatching Quality of Broiler Chicks

Thermal Manipulation: Embryonic Development, Hatchability, and Hatching Quality of Broiler Chicks

Molecular and morphometric changes in the small intestine during hot and cold exposure in thermally manipulated broiler chickens

Examination the effect of post-hatch heat-treatment and heat-stress in Transylvanian Naked Neck chicken

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