HSF-1 activates the ubiquitin proteasome system to promote non-apoptotic developmental cell death in C. elegans

Kinet, Maxime J; Malin, Jennifer A; Abraham, Mary C.; Blum, Elyse S.; Silverman, Melanie; Lu, Yun; Shaham, Shai

doi:10.7554/elife.12821

Cited by 23 publications

(36 citation statements)

References 57 publications

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“…However, there is now increasing evidence from a number of biological systems that HSF1 is important for diverse “non-stress” conditions including development [26, 40], energy metabolism [27], programmed cell death [41, 42] and carcinogenesis [28, 43]. For these non-heat shock conditions, the transcriptomes regulated by HSF1 are distinct from that of acute heat shock [26, 40].…”

Section: Hsf1 Directs Transcriptional Programs That Are Uncoupled Fromentioning

confidence: 99%

Rethinking HSF1 in Stress, Development, and Organismal Health

Labbadia

Morimoto

2017

Trends in Cell Biology

215

210

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The heat shock response (HSR) was originally discovered as a transcriptional response to elevated temperature shock and led to the identification of heat shock proteins and Heat Shock Factor 1 (HSF1). Since then, HSF1 has been shown to be important for combating other forms of environmental perturbations as well as genetic variations that cause proteotoxic stress. The HSR has long been thought to be an absolute response to conditions of cell stress and the primary mechanism by which HSF1 promotes organismal health by preventing protein aggregation and subsequent proteome imbalance. Accumulating evidence now shows that HSF1, the central player in the HSR, is regulated according to specific cellular requirements through cell-autonomous and non-autonomous signals, and directs transcriptional programs distinct from the HSR during development and in carcinogenesis. Here, we discuss these ‘non-canonical’ roles of HSF1, and its regulation in diverse conditions of development, reproduction, metabolism, and aging, and posit that HSF1 serves to integrate diverse biological and pathological responses.

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Section: Hsf1 Directs Transcriptional Programs That Are Uncoupled Fromentioning

confidence: 99%

Rethinking HSF1 in Stress, Development, and Organismal Health

Labbadia

Morimoto

2017

Trends in Cell Biology

215

210

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“…The death of the male linker cell does not involve the ced-3-encoded caspase or other canonical apoptosis pathway genes, but instead uses the heat shock factor HSF-1 and the ubiquitin proteasome system. EGL-20/LIN-44 WNT ligands, LIN-29-mediated transcriptional regulation, and the TIR-1/SEK-1 kinase pathway provide three redundant systems that regulate the positional timing of the male linker cell's death (Abraham et al 2007) (Blum et al 2012;Kinet et al 2016). After the linker cell dies and its corpse is engulfed, one of the proctodeal cells, B.ae(left or right)aav, directly connects the vas deferens' opening to the proctodeal cavity.…”

Section: Sexual Dimorphism In the Hindgut: Sex-specific Development Amentioning

confidence: 99%

Sexual Dimorphism and Sex Differences in Caenorhabditis elegans Neuronal Development and Behavior

2018

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As fundamental features of nearly all animal species, sexual dimorphisms and sex differences have particular relevance for the development and function of the nervous system. The unique advantages of the nematode have allowed the neurobiology of sex to be studied at unprecedented scale, linking ultrastructure, molecular genetics, cell biology, development, neural circuit function, and behavior. Sex differences in the nervous system encompass prominent anatomical dimorphisms as well as differences in physiology and connectivity. The influence of sex on behavior is just as diverse, with biological sex programming innate sex-specific behaviors and modifying many other aspects of neural circuit function. The study of these differences has provided important insights into mechanisms of neurogenesis, cell fate specification, and differentiation; synaptogenesis and connectivity; principles of circuit function, plasticity, and behavior; social communication; and many other areas of modern neurobiology.

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“…The LC is born in the L2 stage and leads elongation of the male gonad during the L3 and L4 stages (Schwarz et al, 2012). The cell undergoes non-apoptotic programmed cell death in the late L4 stage (Blum et al, 2012), and the genetic basis for this has been under recent investigation (Blum et al, 2012; Kinet et al, 2016). Live imaging of LC death has been a major challenge in the field, as imaging over >10 hours is required, often through the L4-adult molt.…”

Section: Introductionmentioning

confidence: 99%

Long-Term High-Resolution Imaging of Developing C. elegans Larvae with Microfluidics

et al. 2017

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Long-term studies of C. elegans larval development traditionally require tedious manual observations as larvae must move to develop, and existing immobilization techniques either perturb development or are unsuited for young larvae. Here, we present a simple microfluidic device to simultaneously follow development of 10 C. elegans larvae at high spatiotemporal resolution from hatching to adulthood (~3 days). Animals grown in micro-chambers are periodically immobilized by compression to allow high-quality imaging of even weak fluorescence signals. Using the device, we obtain cell-cycle statistics for C. elegans vulval development, a paradigm for organogenesis. We combine Nomarski and multi-channel fluorescence microscopy to study processes such as cell-fate specification, cell death, and trans-differentiation throughout postembryonic development. Finally, we generate time-lapse movies of complex neural arborization through automated image registration. Our technique opens the door to quantitative analysis of time-dependent phenomena governing cellular behavior during C. elegans larval development.

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HSF-1 activates the ubiquitin proteasome system to promote non-apoptotic developmental cell death in C. elegans

Cited by 23 publications

References 57 publications

Rethinking HSF1 in Stress, Development, and Organismal Health

Rethinking HSF1 in Stress, Development, and Organismal Health

Sexual Dimorphism and Sex Differences in Caenorhabditis elegans Neuronal Development and Behavior

Long-Term High-Resolution Imaging of Developing C. elegans Larvae with Microfluidics

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