Hsc70 binds to ultraspiracle resulting in the upregulation of 20-hydroxyecdsone-responsive genes in Helicoverpa armigera

Zheng, Weiwei; Yang, Dantong; Wang, Jin-Xing; Song, Qisheng; Gilbert, Lawrence I.; Zhao, Xiao‐Fan

doi:10.1016/j.mce.2009.10.018

Cited by 32 publications

(19 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased Ca 2ϩ and DAG activate PKC to regulate the phosphorylation of USP1 and CDK10 for gene transcription and insect metamorphosis. This chart is based on the models reported by our laboratory, including the ErGPCR-regulated nongenomic action (15), the interaction between Hsc70 and USP1 (57), and the participation of CDK10-Hsp90-Hsc70-EcRB1 in the 20E transcriptional complex (14).…”

Section: Plcg1 Participates In 20e-induced Ca 2ϩmentioning

confidence: 99%

Phospholipase Cγ1 Connects the Cell Membrane Pathway to the Nuclear Receptor Pathway in Insect Steroid Hormone Signaling

Liu

Cai

Zheng

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background: PLCG1 plays an important role in calcium signaling. Results: PLCG1 up-regulates 20E-induced calcium signaling and regulates USP1 PKC phosphorylation in the lepidopteran insect Helicoverpa armigera. Conclusion: 20E activates PLCG1 to induce calcium influx to regulate USP1 PKC phosphorylation for gene expression. Significance: Our study establishes a link between the nongenomic pathway and genomic pathway in steroid hormone 20E signaling.

show abstract

Section: Plcg1 Participates In 20e-induced Ca 2ϩmentioning

confidence: 99%

Phospholipase Cγ1 Connects the Cell Membrane Pathway to the Nuclear Receptor Pathway in Insect Steroid Hormone Signaling

Liu

Cai

Zheng

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…The heat shock proteins Hsp90 and Hsp70 interact with the nuclear receptors to facilitate their DNA binding activity in fruit flies [4] and mammals [5]. Hsp90 [6] and Hsc70 [7] have been found to be involved in insect steroid hormone signaling by differential interaction with the nuclear receptors. However, plant steroid hormones, such as brassinosteroids, employ a nongenomic pathway to initiate signaling by combining with plasma membrane receptor kinases for gene transactivation [8].…”

Section: Introductionmentioning

confidence: 99%

G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane

et al. 2014

Self Cite

View full text Add to dashboard Cite

BackgroundAnimal steroid hormones are conventionally known to initiate signaling via a genomic pathway by binding to the nuclear receptors. The mechanism by which 20E initiates signaling via a nongenomic pathway is unclear.ResultsWe illustrate that 20E triggered the nongenomic pathway through a plasma membrane G-protein-coupled receptor (named ErGPCR) in the lepidopteran insect Helicoverpa armigera. The transcript of ErGPCR was increased at the larval molting stage and metamorphic molting stage by 20E regulation. Knockdown of ErGPCR via RNA interference in vivo blocked larval–pupal transition and suppressed 20E-induced gene expression. ErGPCR overexpression in the H. armigera epidermal cell line increased the 20E-induced gene expression. Through ErGPCR, 20E modulated Calponin nuclear translocation and phosphorylation, and induced a rapid increase in cytosolic Ca2+ levels. The inhibitors of T-type voltage-gated calcium channels and canonical transient receptor potential calcium channels repressed the 20E-induced Ca2+ increase. Truncation of the N-terminal extracellular region of ErGPCR inhibited its localization on the plasma membrane and 20E-induced gene expression. ErGPCR was not detected to bind with the steroid hormone analog [3H]Pon A.ConclusionThese results suggest that ErGPCR participates in 20E signaling on the plasma membrane.

show abstract

“…The cells were cultured for various times after 20E (Sigma, St Louis, MO, USA) or methoprene (Meth, a JH analog) (Sigma, St louis, MO) was added to the cells. The final concentration of both hormones was 1 µM according to previous work [11], [43], [44], [45], [46], [47], [48], [49], [50]. The control cells received an equal volume of dimethylsulfoxide (DMSO), which was used as a solvent for 20E and methoprene.…”

Section: Methodsmentioning

confidence: 99%

“…The final concentration of dsRNA was 3 µg/mL in the medium. After incubation at 26°C for 10 h post dsRNA transfection, the cells were rinsed and then replenished with a medium containing 1 µM 20E for another 12 h of incubation [49]. The RNA was finally extracted from the cells for RT-PCR analysis.…”

Section: Methodsmentioning

confidence: 99%

The Participation of Calponin in the Cross Talk between 20-Hydroxyecdysone and Juvenile Hormone Signaling Pathways by Phosphorylation Variation

et al. 2011

Self Cite

View full text Add to dashboard Cite

20-hydroxyecdysone (20E) and juvenile hormone (JH) signaling pathways interact to mediate insect development, but the mechanism of this interaction is poorly understood. Here, a calponin homologue domain (Chd) containing protein (HaCal) is reported to play a key role in the cross talk between 20E and JH signaling by varying its phosphorylation. Chd is known as an actin binding domain present in many proteins including some signaling proteins. Using an epidermal cell line (HaEpi), HaCal was found to be up-regulated by either 20E or the JH analog methoprene (JHA). 20E induced rapid phosphorylation of HaCal whereas no phosphorylation occurred with JHA. HaCal could be quickly translocated into the nuclei through 20E or JH signaling but interacted with USP1 only under the mediation of JHA. Knockdown of HaCal by RNAi blocked the 20E inducibility of USP1, PKC and HR3, and also blocked the JHA inducibility of USP1, PKC and JHi. After gene silencing of HaCal by ingestion of dsHaCal expressed by Escherichia coli, the larval development was arrested and the gene expression of USP1, PKC, HR3 and JHi were blocked. These composite data suggest that HaCal plays roles in hormonal signaling by quickly transferring into nucleus to function as a phosphorylated form in the 20E pathway and as a non-phosphorylated form interacting with USP1 in the JH pathway to facilitate 20E or JH signaling cascade, in short, by switching its phosphorylation status to regulate insect development.

show abstract

Hsc70 binds to ultraspiracle resulting in the upregulation of 20-hydroxyecdsone-responsive genes in Helicoverpa armigera

Cited by 32 publications

References 41 publications

Phospholipase Cγ1 Connects the Cell Membrane Pathway to the Nuclear Receptor Pathway in Insect Steroid Hormone Signaling

Phospholipase Cγ1 Connects the Cell Membrane Pathway to the Nuclear Receptor Pathway in Insect Steroid Hormone Signaling

G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane

The Participation of Calponin in the Cross Talk between 20-Hydroxyecdysone and Juvenile Hormone Signaling Pathways by Phosphorylation Variation

Contact Info

Product

Resources

About