hsa_circ_0139402 Promotes Bladder Cancer Progression by Regulating hsa-miR-326/PAX8 Signaling

Abstract

Background. Bladder cancer (BC) is a malignant and common malignant tumors. However, the prognosis of most patients with bladder cancer is still poor, and it is particularly important to identify early tumor diagnostic and treatment targets. Materials and Methods. High-throughput sequencing was used to evaluate the expression level of circRNA in bladder cancer tissue. MTT assay, wound healing assay, and transwell assay were used to detect the cancer cells’ proliferation, migration, and invasion affected by hsa_circ_0139402. The possible miRNA targets of hsa_circ_0139402 and downstream genes were detected by bioinformatics methods and dual-luciferase reporting experiment. FISH was used to observe their interaction. Results. High-throughput sequencing result showed that the expression of hsa_circ_0139402 was highest in BC tissues and increased in metastatic tissues compared to that of nonmetastatic tissues. MTT assay, wound healing assay, and transwell assay revealed that sh-hsa_circ_0139402 could suppress BC cells’ proliferation, invasion, and migration. Bioinformatics analysis, dual-luciferase reporter, and RIP assay showed that hsa_circ_0139402 can bind to hsa-miR-326, and PAX8 is a direct target of hsa-miR-326 in BC cell. Further, cytological studies found that hsa_circ_0139402 enhances BC cells’ proliferation, migration, and invasion by targeting PAX8 via hsa-miR-326. Conclusion. hsa_circ_0139402 plays a oncogene in BC and that can effectively promote cell proliferation, migration, invasion, and EMT by targeting Paired Box Protein Pax-8 (PAX8) via hsa-miR-326 and provides a potential therapeutic target for BC patients.

Construction of a Necroptosis-Related miRNA Signature for Predicting the Prognosis of Patients With Hepatocellular Carcinoma

“…Small RNA RNU6B (U6) (RiboBio, Guangzhou, China) was used as a control for the expression of miRNA ( Li et al, 2022a ). Primer sequences are summarized in previous studies, including miR-139-5p ( Li et al, 2022b ), hsa-miR-326 ( Wei et al, 2022 ), miR-10b-5p ( Niu et al, 2021 ), miR-500a-3p ( Long et al, 2022 ), and miR-592 ( Paul et al, 2021 ).…”

Pax‐8: Molecular biology, pathophysiology, and potential pathogenesis

“…52 Moreover, studies have shown that Pax-8 promotes tumor cell proliferation, migration, invasion, and endothelial-mesenchymal transition (EMT) by targeting miR-144-3p and hsa_circ_0139402/hsa-miR-326. 52,53 Collectively, more investigation is necessary to fully comprehend how multiple factors cooperatively regulate Pax-8 expression. The specific molecular mechanism of translational or posttranslational regulation of Pax-8 needs to be further clarified.…”

“…The luciferase assay further demonstrated that miR‐144‐3p directly targets the 3′ untranslated region (3′UTR) of the Pax‐8 gene to suppress its translation 52 . Moreover, studies have shown that Pax‐8 promotes tumor cell proliferation, migration, invasion, and endothelial‐mesenchymal transition (EMT) by targeting miR‐144‐3p and hsa_circ_0139402/hsa‐miR‐326 52,53 . Collectively, more investigation is necessary to fully comprehend how multiple factors cooperatively regulate Pax‐8 expression.…”

NEDD4L inhibits migration, invasion, cisplatin resistance and promotes apoptosis of bladder cancer cells by inactivating the p62/Keap1/Nrf2 pathway

“…Bladder cancer (BLCA) ranks the ninth most common cancer globally, which is the most common malignancy of the urinary tract 1,2 . There are about 550 000 persons are newly diagnosed with BLCA and 200 000 persons died from this unfavorable disease in the world every year 3 .…”