Hsa_circ_0005909 promotes cell proliferation of osteosarcoma cells by targeting miR-338-3p/HMGA1 axis

Abstract

Objective: Osteosarcoma (OS) is the most common malignant bone tumor in pediatric population. The main goal of this study is to investigate the role of has_circ_0005909 and the underlying signaling pathway involved in OS.Methods: Cell proliferation was measured using CCK-8 assay kit and clone formation assay. Change of RNA and protein expression was determined using RNA extract and quantitative real time PCR (RT-qPCR) assay and Western blotting, respectively. CircInteractome was used to predict the target of circRNA and starBase v2.0 was used to predict the target of miRNAs. Luciferase assay was used to confirm the predicted results from CircInteractome and starBase v2.0.Results: Expression of circ_0005909 was upregulated in both OS tissues and cell lines. The predicted results from CircInteractome and starBase v2.0 demonstrated that circ_0005909 could sponge miR-338-3p and that HGMA1 was the direct target of miR-338-3p. Cell viability and cell clones was inhibited by knockdown of circ_0005909 but increased by dual inhibition of circ_0005909 and miR-338-3p. Phosphorylation of ERK, Akt, PI3K was inhibited by sh-circ_0005909 while this inhibition was repressed by co-transfection of sh-circ_0005909 and HGMA1.Conclusion: Expression of circ_0005909 was upregulated in both OS tissues and cell lines which upregulated expression of HGMA1 through sponging miR-338-3p, resulting in the activation of MAPK-ERK and PI3K-Akt signaling pathways to promote the development of OS.