Hsa_circ_0003998 promotes epithelial to mesenchymal transition of hepatocellular carcinoma by sponging miR-143-3p and PCBP1

Song, Lina; Qiao, Guanglei; Yu, Jian; Yang, Chunmei; Chen, Ying; Deng, Zhoufeng; Song, Lihua; Ma, Lijun; Yan, Hongli

doi:10.1186/s13046-020-01576-0

Cited by 63 publications

(47 citation statements)

References 42 publications

(43 reference statements)

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“…Acting as competing endogenous RNAs (ceRNAs), lncRNAs may binding to specific miRNAs to suppress target mRNA genes expression 20 . To verify EIF3J-AS1 acted as ceRNA in Eca, we performed Nuclear/cytoplasmic fraction assay and Fig.…”

Section: Resultsmentioning

confidence: 99%

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LncRNA EIF3J-AS1 enhanced esophageal cancer invasion via regulating AKT1 expression through sponging miR-373-3p

Wei

Wang

Liang

et al. 2020

Sci Rep

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Esophageal cancer (ECa) remains a major cause of mortality across the globe. The expression of EIF3J-AS1 is altered in a plethora of tumors, but its role in ECa development and progression are undefined. Here, we show that EIF3J-AS1 is up-regulated in ECa and that its expression correlates with advanced TNM stage (P = 0.014), invasion depth (P = 0.001), positive lymph node metastasis (P < 0.001) and poor survival (OS: P = 0.0059; DFS: P = 0.0037) in ECa. Functional experiments showed that knockdown EIF3J-AS1 inhibited ECa growth and metastasis through in vitro and in vivo experiments. Regarding the mechanism, EIF3J-AS1/miR-373-3p/AKT1 established the ceRNA network involved in the modulation of cell progression of ECa cells. Overall, EIF3J-AS1 may exhibit an oncogenic function in ECa via acting as a sponge for miR-373-3p to up-regulate AKT1 mRNA level, and may serve as a potential therapeutic target and a prognostic biomarker for ECa patients. Esophageal cancer (ECa) is of particularly high incidence in Chinese males compared to other regions and remains a global threat to human health. Up to ~ 450,000 new cases of ECa are diagnosed annually, of which up to ~ 400,000 of those afflicted do not survive 1. Diagnostic and therapeutic advances have been made to improve ECa therapeutics, including biopsies, chromo endoscopy and narrow-band imaging 2. However, despite these improvements, the efficiency of ECa therapies and subsequent patient prognosis remains poor. ECa therefore remains a global health burden for which new diagnostics and effective therapeutic strategies are urgently required 3. A further understanding of the molecular regulators of ECa tumorigenesis is required to expedite the discovery of novel anti-ECa strategies. While lncRNAs (long noncoding RNAs; more than 200 bases long 4-8 are not capable of translating proteins, their dysregulation is observed several cancers, such as ECa 9-12. For instance, the process of EMT (epithelialmesenchymal transition) is enhanced by lncRNA PVT1 via E-cadherin suppression in ECa 13. Further, significantly enhanced level of CASC11 was observed in ECa tissues while its silencing impeded the progression of ECa by regulating KLF6 expression 14. Previous study reported that up-regulated a novelty EIF3J-AS1 was associated with poor survival and accelerated HCC progression via targeting miR-122e5p/CTNND2 axis under hypoxia 15. Meanwhile, EIF3J-AS1 induced by H3K27 promoted colorectal cancer proliferation and inhibited apoptosis via miR-3163/YAP1 axis 16. Nevertheless, the role and inherent mechanisms of EIF3J-AS1 in human ECa development remains to be assessed. In this research, we examined the increased expression of EIF3J-AS1 in ECa cell lines and tissues, the effect of EIF3J-AS1 knocked down on inhibiting the metastasis and growth of ECa cell lines in vitro and vivo. We also examined the role of EIF3J-AS1 in ECa cells' aggressive phenotypes by miR-373-3p binding and assessing the AKT1 expression. Materials and methods Tissue specimens. Collection of a total of 182 ECa cases...

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Section: Resultsmentioning

confidence: 99%

“…In vivo assessments were performed as previous 20 in male nude mice aged 6 weeks (Beijing Vitonlihua Experimental Animal Technology Co. Ltd, Beijing, China). Animals were housed in specified cages that were approved by the national animal guidelines of our institute.…”

Section: Methodsmentioning

confidence: 99%

LncRNA EIF3J-AS1 enhanced esophageal cancer invasion via regulating AKT1 expression through sponging miR-373-3p

Wei

Wang

Liang

et al. 2020

Sci Rep

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“…In addition, PCBP1 could also regulate alternative splicing of CD44 and inhibit invasion in human HepG2 cells 8 . Notably, circ0003998 could interact with PCBP1 to increase the expression level of CD44v6 and promote the EMT in hepatocellular carcinoma 55 . Taken together, PCBP1 acts as a splicing factor to participate in alternative splicing of multiple pre‐mRNA.…”

Section: Pcbp1 Regulates Gene Expression From Different Levelsmentioning

confidence: 96%

“…8 Notably, circ0003998 could interact with PCBP1 to increase the expression level of CD44v6 and promote the EMT in hepatocellular carcinoma. 55 Taken together, PCBP1 acts as a splicing factor to participate in alternative splicing of multiple pre-mRNA. Importantly, it may also bring a novel thought for elucidating the mechanism of tumorigenesis, development, and metastasis.…”

Section: Pcbp1 Regulates Alternative Splicingmentioning

confidence: 99%

Multilevel regulation and molecular mechanism of poly (rC)‐binding protein 1 in cancer

Zhang

Chen

et al. 2020

FASEB j.

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Poly (rC)-binding protein 1 (PCBP1), an RNA-or DNA-binding protein with a relative molecular weight of 38 kDa, which is characterized by downregulation in many cancer types. Numerous cases have indicated that PCBP1 could be considered as a tumor suppressor to inhibit tumorigenesis, development, and metastasis. In the current review, we described the multilevel regulatory roles of PCBP1, including gene transcription, alternative splicing, and translation of many cancer-related genes. Additionally, we also provided a brief overview about the inhibitory effect of PCBP1 on most common tumors. More importantly, we summarized the current research status about PCBP1 in hypoxic microenvironment, autophagy, apoptosis, and chemotherapy of cancer cells, aiming to clarify the molecular mechanisms of PCBP1 in cancer. Taken together, in-depth study of PCBP1 in cancer may provide new ideas for cancer therapy.

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“…Circβ-catenin encodes a novel β-catenin isoform that promotes liver cancer growth and migration in vitro and attenuates tumorigenesis and metastasis in vivo by activating the Wnt pathway. A study by Song et al [ 68 ] showed that circ0003998 was markedly upregulated in portal vein tumor thrombus (PVTT) metastasis and HCC tissues. Its expression was related to the aggressive characteristics of HCC patients.…”

Section: The Characteristics and Categories Of Circrnasmentioning

confidence: 99%

Cancer-related circular RNA: diverse biological functions

et al. 2021

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Noncoding RNAs, including long noncoding RNAs (lncRNAs), microRNAs (miRNAs) and circular RNAs (circRNAs), are involved in regulating biological functions. In recent decades, miRNAs and lncRNAs have both inspired a wave of research, but the study of circRNA functions is still in its infancy. Studies have found that circRNAs actively participate in the occurrence and development of various diseases, which emphasizes the importance of circRNAs. Here, we review the features and classification of circRNAs and summarize their functions. Then, we briefly describe how to analyze circRNAs by bioinformatics procedures. In addition, the relationship between circRNAs and cancers is discussed with an emphasis on proving whether circRNAs can be potential biomarkers for the prognosis and diagnosis of cancer.

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Hsa_circ_0003998 promotes epithelial to mesenchymal transition of hepatocellular carcinoma by sponging miR-143-3p and PCBP1

Cited by 63 publications

References 42 publications

LncRNA EIF3J-AS1 enhanced esophageal cancer invasion via regulating AKT1 expression through sponging miR-373-3p

LncRNA EIF3J-AS1 enhanced esophageal cancer invasion via regulating AKT1 expression through sponging miR-373-3p

Multilevel regulation and molecular mechanism of poly (rC)‐binding protein 1 in cancer

Cancer-related circular RNA: diverse biological functions

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