Hsa_circ_0000437 upregulates and promotes disease progression in rheumatic valvular heart disease

Zhu, Linwen; Wang, Zhifang; L, Sun; Zheng, Dawei; Hu, Bingchuan; Li, Ni; Shao, Guofeng

doi:10.1002/jcla.24197

Cited by 8 publications

(8 citation statements)

References 39 publications

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“…Total RNA extraction using the TRIzol LS reagent (Invitrogen) and reverse transcription were performed as previously described. 10 ABI 7500 System (Applied Biosystems, USA). GAPD H was used as the reference gene.…”

Section: Methodsmentioning

confidence: 99%

“…Data analysis was done using R software limma package. The circRNA array data were as previously described 10 . Differentially expressed circRNAs changes were identified with a fold change of >2.0 and p ‐value <0.05 as significant.…”

Section: Methodsmentioning

confidence: 99%

“… 9 However, recent studies showed that circRNA acts as a micro‐RNA (miRNA) sponge to regulate gene expression in several ways. 10 Many studies demonstrate that miRNA plays an essential regulatory role in tumorigenesis and development. 11 , 12 Based on the function of circRNA to sponge adsorb miRNA, circRNA plays a minor role in regulating tumorigenesis and development.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike linear RNA, the structure of circRNA is stable and not susceptible to degradation by nuclease 9 . However, recent studies showed that circRNA acts as a micro‐RNA (miRNA) sponge to regulate gene expression in several ways 10 . Many studies demonstrate that miRNA plays an essential regulatory role in tumorigenesis and development 11,12 .…”

Section: Introductionmentioning

confidence: 99%

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Upregulation of hsa_circ_0000977 participates in esophageal squamous cancer progression by sponging miR‐874‐3p

et al. 2022

Clinical Laboratory Analysis

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Background Esophageal squamous cell carcinoma (ESCC) is one of the most common clinical malignancies of the digestive system, characterized by high mortality but not evident early symptoms. Molecular markers for diagnostic and outcome prediction are urgently needed. Circular RNAs might play essential roles in the progression of ESCC. Methods Hsa_circ_0000977 was identified using circRNA microarrays and qRT‐PCR. The diagnostic value of hsa_circ_0000977 was calculated. We also examined in vitro cell functions in ECA109 and TE12 ESCC cells to determine the effect of hsa_circ_0000977. A dual‐luciferase reporter vector validated the binding of hsa_circ_0000977 to miR‐874‐3p. Results The top 10 significantly upregulated circRNAs from microarray assays were hsa_circ_0000977, hsa_circ_0006220, hsa_circ_0043278, hsa_circ_0000691, hsa_circ_0000288, hsa_circ_0000367, hsa_circ_0021647, hsa_circ_0006440, hsa_circRNA_405571 and hsa_circRNA_100790, while the top 10 significantly downregulated circRNAs were hsa_circ_0008389, hsa_circ_0089763, hsa_circ_0089762, hsa_circ_0000102, hsa_circ_0001714, hsa_circ_0089761, hsa_circ_0007326, hsa_circ_0001549, hsa_circ_0005133 and hsa_circRNA_405965. Hsa_circ_0000977 was significantly upregulated in ESCC ( p < 0.01) and had diagnostic value in ESCC. The hsa_circ_0000977 expression level was related to the pT stage and numbers of lymph nodes in ESCC patients. Elevated hsa_circ_0000977 promoted cell proliferation, migration and inhibited apoptosis in ESCC cells. Hsa_circ_0000977 might function as a micro‐RNA sponge to competitively bind miR‐874‐3p. Conclusion Disordered hsa_circ_0000977 expression can promote carcinogenesis in ESCC and might serve as a diagnostic biomarker to evaluate the occurrence and development of esophageal cancer.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Upregulation of hsa_circ_0000977 participates in esophageal squamous cancer progression by sponging miR‐874‐3p

et al. 2022

Clinical Laboratory Analysis

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“…In vitro experiments showed that hsa_circ_0000437 promoted proliferation and migration but inhibited apoptosis in RVHD cells. 20 There is abnormal expression of circRNAs in patients with valvular heart disease complicated with atrial fibrillation. Investigators studied expression profiles of circRNAs, laying the foundation for future circRNA‐related research.…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0003748 promotes disease progression in rheumatic valvular heart disease by sponging miR‐577

Zhang

Yuan-fu

et al. 2022

Clinical Laboratory Analysis

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The diagnosis and treatment of rheumatic valvular heart disease (RVHD) require substantial improvements. Studies found that circular RNAs (circRNAs) are involved in the progression of cardiovascular diseases. We screened target hsa_circ_0003748 by circRNA microarrays uploaded to a database. We used fluorescence in situ hybridization to determine the cellular location of hsa_circ_0003748. A dual‐luciferase reporter gene assay revealed that has_circ_0003748 might bind the miRNA miR‐577. In hVIC cells (an RVHD cell line), Cell Counting Kit‐8, Transwell, and flow cytometry assays measured proliferation, migration, and cell cycle and apoptosis, respectively. We found that hsa_circ_0003748 was localized in the cytoplasm; hsa_circ_0003748 promoted the proliferation and migration of hVIC cells, arrested the cell cycle in the G2/M phase, and inhibited apoptosis. These phenomena may result from hsa_circ_0003748 promoting RVHD after sponging miR‐577. Bioinformatic analysis revealed that hsa_circ_0003748 might affect RVHD progression by affecting transcription and the MAPK signaling pathway, the Ras signaling pathway, the cAMP signaling pathway, the Rap1 signaling pathway, and other signaling pathways.

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Let‑7f‑5p Regulated by Hsa_circ_0000437 Ameliorates Bleomycin‐Induced Skin Fibrosis

Liu,

Li,

et al. 2024

J of Cellular Biochemistry

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The current treatment of skin fibrosis is limited in its effectiveness due to a lack of understanding of the underlying mechanisms. Previous research has shown a connection between microRNAs (miRNAs) and the development of skin fibrosis. Therefore, investigating miRNA for the treatment of skin fibrotic diseases is highly important and merits further exploration. In this study, we have discovered that let‐7f‐5p could suppress the proliferation, migration, and expression of collagen type I alpha 1 (COL1A1) in human dermal fibroblasts (HDFs). It was further determined that let‐7f‐5p could target thrombospondin‐1 (THBS1), thereby inhibiting the TGF‐β2/Smad3 signaling pathway and exerting its biological effects. Additionally, let‐7f‐5p is regulated by Hsa_circ_0000437, which acts as a sponge molecule for let‐7f‐5p and consequently regulates the biological function of HDFs. Furthermore, our findings indicate that in vivo overexpression of let‐7f‐5p leads to a reduction in dermal thickness and COL1A1 expression, effectively inhibiting the progression of bleomycin (BLM)‐induced skin fibrosis in mice. Hence, our research enhances the comprehension of the Hsa_circ_0000437/let‐7f‐5p/THBS1/TGF‐β2/Smad3 regulatory network, highlighting the potential of let‐7f‐5p as a therapeutic approach for the treatment of skin fibrosis.

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Hsa_circ_0000437 upregulates and promotes disease progression in rheumatic valvular heart disease

Cited by 8 publications

References 39 publications

Upregulation of hsa_circ_0000977 participates in esophageal squamous cancer progression by sponging miR‐874‐3p

Upregulation of hsa_circ_0000977 participates in esophageal squamous cancer progression by sponging miR‐874‐3p

Hsa_circ_0003748 promotes disease progression in rheumatic valvular heart disease by sponging miR‐577

Let‑7f‑5p Regulated by Hsa_circ_0000437 Ameliorates Bleomycin‐Induced Skin Fibrosis

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