hsa‑microRNA‑411‑5p regulates proliferation, migration and invasion by targeting the hyaluronan mediated motility receptor in ovarian cancer

Abstract

The mortality rate of ovarian cancer is the highest out of all gynecological malignancies worldwide. Therefore, it is important to understand the mechanisms of ovarian cancer, identify new biomarkers and develop targeted drugs. The role and molecular mechanisms of hsa-microRNA (miR)-411-5p in ovarian cancer have not been fully elucidated. The present study investigated the ovarian cancer cell lines OVCAR-8 and SKOV3. After transfection with miRNA mimics, cell proliferation was monitored by a proliferation assay. Furthermore, cell migration was measured by a cell wound healing assay and cell invasion was measured by Matrigel invasion assays. A miRNA luciferase reporter assay was used to analyze the relationship between miRNAs and the target gene HMMR, which was then further evaluated by gene differential analysis. In the current study, hsa-mir-411-5p was identified as a miRNA regulator of the hyaluronan mediated motility receptor, which negatively regulated the activity of ERK1/2 and ultimately inhibited ovarian cancer cell proliferation and motility. Although hsa-mir-411-5p may have different roles in other types of cancer, the present study suggested that miR-411-5p functions as a negative tumor regulator in ovarian cancer cells, displaying the potential of miR-411-5p as a biomarker for ovarian cancer.

LncRNA ST8SIA6-AS1 Promotes Cholangiocarcinoma Progression by Suppressing the miR-145-5p/MAL2 Axis

“…16 Growing evidence has indicated that miRNAs can regulate the progression of various cancers by regulating cell growth, migration, invasion, and apoptosis, such as pancreatic cancer, papillary thyroid cancer, and ovarian cancer. [17][18][19] For instance, miR-133a-3p repressed the growth and metastasis of gastric cancer cells via blocking autophagymediated glutaminolysis. 20 MiRNA-106a has been confirmed to promote breast cancer cells' proliferation, clonogenicity, migration, and invasion but inhibit their apoptosis and chemosensitivity.…”

Hsa_circ_0005273 acts as a sponge of miR‐509‐3p to promote the malignant behaviors of breast cancer by regulating HMMR expression

“… 28 HMMR is associated with BC risk and cancer progression across multiple tumor types. 29 , 30 HMMR has been reported to be resistant to chemotherapy by inducing epithelial‐mesenchymal transformation in gastric cancer through the TGFβ/Smad2 pathway. 31 Existing evidence has revealed that HMMR is often freakishly expressed in human tumors and its overexpression might accelerate the formation and progression of different cancers, such as lung adenocarcinoma 32 and colon cancer.…”

“…HMMR is one of the few defining receptors for hyaluronan and was a centrosomal and microtubule‐associated protein that regulates cell growth 28 . HMMR is associated with BC risk and cancer progression across multiple tumor types 29,30 . HMMR has been reported to be resistant to chemotherapy by inducing epithelial‐mesenchymal transformation in gastric cancer through the TGFβ/Smad2 pathway 31 .…”

Circ_0076305 facilitates prostate cancer development via sponging miR‐411‐5p and regulating PGK1

“…Former researchers have revealed that miR‐411‐5p is able to serve as tumour‐suppressing miRNA in several tumours. For examples, miR‐411‐5p could target HMMR to play a suppressing role in ovarian cancer (He et al, 2020). Moreover, miR‐411‐5p decreased GRB2 to limit breast cancer cell metastasis and proliferation (Zhang et al, 2016).…”