Hsa-let-7e-5p Inhibits the Proliferation and Metastasis of Head and Neck Squamous Cell Carcinoma Cells by Targeting Chemokine Receptor 7

Wang, Song; Jin, Shan; Liu, Minda; Pang, Pai; Wu, Hong; Qi, Zhong-zheng; Liu, Fa‐Yu; Sun, Changfu

doi:10.7150/jca.29536

Cited by 37 publications

(26 citation statements)

References 25 publications

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“…According to GLOBOCAN 2012, the age-standardized incidence of HNSCC in China is estimated to be 2.7 per 100,000 people 5. Despite advances in diagnosis and treatment, the 5-year survival rate of patients with HNSCC remains poor due to its high propensity for local and distant metastasis 6. The initiation and progression of HNSCC are linked to multiple risk factors including HPV, diet, detrimental habits, genetic background and geographic factors;7 however, the underlying molecular mechanisms are unclear.…”

Section: Introductionmentioning

confidence: 99%

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

Chen¹,

Zhang²,

Sun³

et al. 2019

OTT

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BackgroundHead and neck squamous cell carcinoma (HNSCC) is among the most common malignant tumors worldwide. This study, investigated the role of microRNA (miR)-762 in regulating HNSCC progression.Materials and methodsThe expression levels of miR-762 in HNSCC tissues were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Statistical analyses were performed to investigate the association of miR-762 with clinicopathological features in patients with HNSCC. Cell proliferation and migration were examined by cell counting (CCK-8) and IncuCyte assays. Target genes of miR-762 were screened using bioinformatics tools and microarrays, and confirmed using a luciferase activity reporter assay, qRT-PCR and Western blot analysis. Recuse experiments were performed to detect whether target genes mediated the effects of miR-762 on HNSCC cells. The in vivo effects of miR-762 were verified using tumor xenografts.ResultsHNSCC clinical specimens showed high expression levels of miR-762, which positively correlated with tumor-node-metastasis (TNM) stage and poor prognosis of HNSCC. miR-762 overexpression promoted the proliferation and migration of HNSCC cells in vitro. In addition, overexpression of miR-762 upregulated the expression of phosphorylated AKT (p-AKT) and mesenchymal markers (N-cadherin and vimentin), but suppressed epithelial marker (E-cadherin) expression. miR-762 also promoted HNSCC tumor growth in vivo. PH domain and leucine-rich repeat protein phosphatase 2 (PHLPP2) and Forkhead box O4 (FOXO4) were direct target genes of miR-762. HNSCC tissues had low expression levels of PHLPP2 and FOXO4, showing a negative correlation with miR-762 expression. Moreover, silencing of PHLPP2 and FOXO4 mimicked the tumor-promotive effects of miR-762 on HNSCC cells. Notably, overexpression of PHLPP2 and FOXO4 abolished the pro-tumoral function of miR-762 on cell proliferation and migration.ConclusionmiR-762 promotes HNSCC progression by targeting PHLPP2 and FOXO4. Therefore, miR-762 might be a potential diagnostic or therapeutic target for HNSCC.

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Section: Introductionmentioning

confidence: 99%

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

Chen¹,

Zhang²,

Sun³

et al. 2019

OTT

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“…32 In addition, let-7e-5p overexpression inhibited cell proliferation, wound healing, migration, and invasion in head and neck squamous cell carcinoma cells. 33 Chiam et al found that circulating serum exosomal let-7e-5p may be a useful marker to distinguish esophageal adenocarcinoma from controls and Barrett's esophagus. 34 We supposed that circMYC may play a role in the function of downstream target genes by sponging miR-20b-5p and let-7e-3p.…”

Section: Discussionmentioning

confidence: 99%

“…Recent study also reveals that miR‐20b‐5p is sponged by circRNA DNA Methyltransferase 3 Beta (DMNT3B) in human retinal microvascular endothelial cell 32 . In addition, let‐7e‐5p overexpression inhibited cell proliferation, wound healing, migration, and invasion in head and neck squamous cell carcinoma cells 33 . Chiam et al found that circulating serum exosomal let‐7e‐5p may be a useful marker to distinguish esophageal adenocarcinoma from controls and Barrett's esophagus 34 .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of exosomal circMYC in radioresistant nasopharyngeal carcinoma

Luo

Liu

et al. 2020

Head & Neck

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Background: The relationship between circulating exosomal circular RNA (circRNA) and prognosis of patients with nasopharyngeal carcinoma (NPC) remain unknown. This study focused on the expression of exosomal circMYC and its relationship with the recurrence and prognosis of patients with NPC. Methods: The circulating exosomes were obtained from 210 patients with NPC. Quantitative polymerase chain reaction, 5-ethynyl-2 0-deoxyuridine (EdU) staining, colony formation, and bioinformatic analysis were performed. Results: Circulating exosomal circMYC was significantly increased in patients with NPC and was associated with tumor size, lymph node metastasis, TNM stage, survival rate, and disease recurrence. Gain-functional and lossfunctional experiments revealed that overexpression of circMYC promoted cell proliferation and reduce radiosensitivity, while knockdown of circMYC inhibited cell proliferation and enhanced radiotherapy. Conclusion: circMYC is an oncogene in NPC cells and can enhance the radiotherapy resistance of NPC cells. Circulating exosomal circMYC can be used as a potential therapeutic target for NPC.

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“…reported that CCR7 could also be involved in the proliferation of head and neck squamous cell carcinoma [25]. Besides, Liu et al showed that CCR7 could promote dendritic cell migration by increasing glycolysis metabolism, and there was a negative feedback regulation [26].…”

Section: Tsccmentioning

confidence: 99%

Long noncoding RNA UCA1 regulates CCR7 expression to promote tongue squamous cell carcinoma progression by sponging miR-138-5p

Shi¹,

Li²,

Zhao³

2021

neo

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Tongue squamous cell carcinoma (TSCC) is a malignant tumor. Long non-coding RNAs (lncRNAs) have been proved to be involved in the regulation of the progression of various cancers. However, the mechanism of lncRNA urothelial cancer-associated 1 (UCA1) in the progression of TSCC remains unclear. The expression levels of UCA1, microRNA-138-5p (miR-138-5p), and CC chemokine receptor 7 (CCR7) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation, migration and invasion were detected using colony formation assay and transwell assay, respectively. Western blot (WB) analysis was used to test the levels of proliferation and metastasis-related proteins and CCR7 protein. Moreover, the extracellular acidification rate (ECAR) of cells was measured by the Seahorse XF Extracellular Flux Analyzer, and the adenosine triphosphate (ATP) level, glucose uptake, and lactate produce of cells were tested by their corresponding assay kits. Further, the dual-luciferase reporter assay was used to confirm the interaction between miR-138-5p and UCA1 or CCR7. In addition, the effect of UCA1 on TSCC tumor growth in vivo was evaluated by animal experiments. We found that UCA1 and CCR7 were upregulated, while miR-138-5p was downregulated in TSCC tissues. Silenced UCA1 restrained the proliferation, migration, invasion, and glycolysis metabolism of TSCC cells. Similarly, knockdown of CCR7 also could suppress the progression of TSCC. Besides, UCA1 overexpression promoted TSCC progression, while this promotion effect could be reversed by CCR7 silencing. miR-138-5p could be sponged by UCA1 and could target CCR7. Additionally, miR-138-5p overexpression could reverse the promotion effect of overexpressed UCA1 on TSCC progression. Furthermore, the UCA1 knockdown reduced TSCC tumor growth in vivo. In conclusion, lncRNA UCA1 might function as an oncogene in TSCC through regulating the miR-138-5p/CCR7 axis, providing a new biomarker for TSCC treatment.

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Hsa-let-7e-5p Inhibits the Proliferation and Metastasis of Head and Neck Squamous Cell Carcinoma Cells by Targeting Chemokine Receptor 7

Cited by 37 publications

References 25 publications

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

Diagnostic value of exosomal circMYC in radioresistant nasopharyngeal carcinoma

Long noncoding RNA UCA1 regulates CCR7 expression to promote tongue squamous cell carcinoma progression by sponging miR-138-5p

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