HPV16 E6 Promotes Breast Cancer Proliferation via Upregulation of COX-2 Expression

Wang, Y X; Li, Y Z; Zhang, Z Y; Wang, J Q; Cui, Juan; Qian, Xiangyang

doi:10.1155/2017/2948467

Cited by 10 publications

(6 citation statements)

References 42 publications

(31 reference statements)

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“…Cyclo-oxygenase-2 (COX-2) is a key enzyme in eicosanoid biosynthesis. In previous studies, upregulated COX-2 expression has been observed in a number of malignant tumors, such as breast cancer and colon cancers 4,5 . The overexpression of COX-2 is related to prostaglandin-stimulating angiogenesis and proliferation, which can promote cell invasion and the development of metastases 6 .…”

Section: Introductionmentioning

confidence: 88%

TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway

Zhang

Chen

et al. 2019

Cell Death Dis

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Thyroid cancer is commonly seen in the clinic with a rapidly increasing incidence globally. COX-2 overexpression correlates with the pathologic type of thyroid carcinoma, and it has been suggested that COX-2 overexpression is associated with a poor prognosis. However, little is known about its upstream regulatory mechanism. Bioinformatics suggested that transcription factor AP-2 beta (TFAP2B) might specifically bind to the COX-2 promoter, which was confirmed by biotin-labeled COX-2 promoter pulldown and luciferase reporter assays. We performed western blot and immunohistochemical staining to detect the expression of TFAP2B/COX-2 in thyroid cancer tissues (T) and the matched adjacent noncarcinoma tissues (ANT), and investigated the relationship between TFAP2B/COX-2 expression and clinical pathological factors in thyroid cancer patients. Afterward, MTS, colony formation, cell-apoptosis assay, transwell-invasion and scratch assays were performed to examine the proliferation, apoptosis, invasion, and migration of thyroid cancer cells with TFAP2B knocked down or overexpressed. The mouse xenograft experiment was performed to study in vivo the proliferation of thyroid cancer cells with TFAP2B knocked down or overexpressed. We found that TFAP2B bound to the promoter of COX-2 to activate its expression. Western blot and immunohistochemistry showed that TFAP2B/COX-2 was highly expressed in thyroid cancer, and high TFAP2B and COX-2 expression was associated with aggressive clinicopathological features in thyroid cancer. TFAP2B mediated thyroid cancer cell proliferation, apoptosis, invasion, and migration via the COX-2 signaling pathway in vitro and in vivo. TFAP2B bound to the promoter of COX-2 to activate its expression, indicating that TFAP2B is a critical regulatory molecule in the COX-2 signaling pathway that promoted tumor progression in thyroid cancer.

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Section: Introductionmentioning

confidence: 88%

TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway

Zhang

Chen

et al. 2019

Cell Death Dis

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“…Under inflammatory conditions, iNOS and COX‐2 protein expression are elevated, which facilitates the generation of NO and Prostaglandin F2 alpha (PGF 2α ). iNOS is a target of inflammation‐associated tissue damage (Southan & Szabo, ), and COX‐2 is a mediator of inflammation, angiogenesis, and cancer progression (Wang et al, ). Also, cytokines that include TNF‐α and IL‐6 are related to the inflammation process and known to typical proinflammatory cytokine with tumor growth effect (Landskron, De la Fuente, Thuwajit, Thuwajit, & Hermoso, ).…”

Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory effect from extracts of Red Chinese cabbage and Aronia in LPS‐stimulated RAW 264.7 cells

Kwak

Kim

Ryu

et al. 2020

Food Science & Nutrition

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A chronic inflammatory environment facilitates tumor growth and proliferation. Fruits and vegetables are important sources of anthocyanins, polyphenols, and other biologically active substances that can favorably affect the pathogenesis of cancer. The objective of the study was to investigate the anti‐inflammatory effects of Red Chinese cabbage (RC) and mixture of commercial Red Chinese cabbage leaves and Aronia fruits (ARC) in LPS‐stimulated RAW 264.7 cells. The RAW 264.7 cells were cultured and measured the cytotoxicity by using an MTT assay. The inflammatory markers, such as nitrite, IL‐6, and TNF‐alpha expression, were evaluated using ELISA, and protein expression of inflammatory markers like iNOS and COX‐2 was analyzed using Western blot. MTT assays showed that pretreatment of RAW 264.7 cells with RC and ARC did not change cell growth or cytotoxicity. We also found that ARC extracts reduced inflammation‐related biomarker (TNF‐a, IL‐6, and NO) production and gene expression (iNOS, COX‐2). Our results suggested that ARC has good anti‐inflammatory properties compared with RC that maybe used as potential nutrients for treating inflammatory diseases.

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“…We could not identify any previous epidemiologic studies that have assessed the impact of nonaspirin NSAID use and risk of HNC in relation to HPV, and preclinical evidence concerning the association between HPV and COX‐2 expression is limited and inconsistent. Studies on cervical cancer cell lines and tissue have found upregulation of COX‐2 expression in HPV‐positive compared to HPV‐negative tumor cells, while others reported no correlation . In HNC, one study found increased expression of COX‐2 in an HPV‐transformed dysplastic cell line, while two small studies found no or an inverse association between COX‐2 expression and HPV positivity in human HNC samples …”

Section: Discussionmentioning

confidence: 99%

Use of nonaspirin nonsteroidal anti‐inflammatory drugs and risk of head and neck cancer: A nationwide case–control study

Cour

Dehlendorff

Aalborg

et al. 2019

Intl Journal of Cancer

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Head and neck cancer (HNC) is the sixth most frequent malignancy with high mortality and substantial morbidity and hence there is a need for identification of preventive factors. Preclinical and observational studies have reported antineoplastic effects of nonaspirin nonsteroidal anti‐inflammatory drugs (NSAIDs), but studies of nonaspirin NSAID use and risk of HNC are sparse and with inconsistent results. We therefore conducted a register‐based case–control study nested in the entire Danish population. Cases (n = 12,389) comprised all Danish residents aged 30–84 years with a histologically verified primary HNC diagnosis during 2000–2015. Based on the literature, cases were categorized into four groups of anticipated association with human papillomavirus (HPV): strong, potential, no/weak and uncertain. Age‐ and sex‐matched population controls (n = 185,835) were selected by risk‐set‐sampling. We obtained information on filled prescriptions of nonaspirin NSAIDs, other drug use, comorbid conditions and socioeconomic parameters from nationwide Danish registries. Ever‐use (≥2 prescriptions) of nonaspirin NSAIDs was not associated with the overall risk of HNC after adjustment for potential confounders (odds ratio [OR]: 0.99, 95% confidence interval [CI]: 0.95–1.03). However, long‐term consistent use (≥5 years) was associated with a 25% reduction in HNC risk (OR: 0.75, 95% CI: 0.62–0.90). Stratified analyses by anticipated HPV‐association showed no material differences in estimates. In conclusion, ever‐use of nonaspirin NSAIDs was not associated with the risk of HNC with no apparent influence on the estimates by the anticipated HPV‐association. However, long‐term consistent use may be associated with a reduced risk of HNC and merits further investigation.

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HPV16 E6 Promotes Breast Cancer Proliferation via Upregulation of COX-2 Expression

Cited by 10 publications

References 42 publications

TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway

TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway

Anti‐inflammatory effect from extracts of Red Chinese cabbage and Aronia in LPS‐stimulated RAW 264.7 cells

Use of nonaspirin nonsteroidal anti‐inflammatory drugs and risk of head and neck cancer: A nationwide case–control study

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