HPV‐negative and HPV‐positive HNSCC cell lines show similar numerical but different structural chromosomal aberrations

Abstract

Background It was tested whether the difference in carcinogenesis between noxa and human papillomavirus (HPV)‐driven head and neck squamous cell carcinoma (HNSCC) is associated with a variation in genomic instability. Methods Conventional and molecular cytogenetics in HPV‐positive and HPV‐negative HNSCC cell lines. Results Numerical aneuploidy determined by multicolor fluorescence in situ hybridization and DNA ploidy was very similar for both entities with most chromosomes being present either in quadruplicate or triplicate, and only few are still diploid with, however, a striking similarity in the overall pattern. A clear difference was seen concerning the translocations formed, with no difference in the total amount but with a significantly higher genomic instability of HPV‐positive cell lines at chromosome 3 as compared to HPV‐negative cells. Conclusion The different processes of carcinogenesis of HPV‐positive and HPV‐negative HNSCC appear to result in a similar pattern of numerical but a clear difference in structural chromosomal aberrations.

TP53 and PTEN as driver mutations in Zenker's carcinoma—a clinical presentation

“…Our results indicate genetic similarities to HPV (‐) HNSCC. HPV (+) and HPV (‐) HNSCC seem to have different processes of carcinogenesis 22 which factor may explain why Feldman et al found TP53 as the most frequently mutated gene in HNSCC with 41%, but without any occurrence of the mutation in the HPV (+) carcinomas, and with an occurrence of 57% in HPV (‐) tumors 10 . Similarly, according to the Cancer Genome Atlas Network TP53 mutation occurred in 86% among the HPV (‐) HNSCC samples and only in one case of 36 among HPV (+) samples 11 .…”

“…Alcohol is an established risk factor in HPV (‐) HNSCC 22 , and the association seems to be even stronger among cancers of the oropharynx and hypopharynx 26 . A prolonged exposition of the mucosa due to prolonged washout in Zenker's diverticulum suggests that alcohol may also be a potential risk factor in this condition.…”

Single-Cell Transcriptome Analysis Reveals Different Immune Signatures in HPV- and HPV + Driven Human Head and Neck Squamous Cell Carcinoma

“…Accordingly, several molecular differences exist between HPV-positive and HPV-negative tumors, including higher frequency of intratumoral B cells in HPV-positive [7] and higher frequency of dysfunctional CD8+ T cells in HPV-negative patients [8]. Furthermore, tumors with HPV-positive entity show a similar pattern of numerical but a difference in structural chromosomal aberrations compared to HPV-negative ones [9]. While HPVnegative HNSCC often show mutations in genes like TP53 and CDKN2A, which is primarily associated to tobacco, HPV-positive tumors represent an increased expression of the E6 and E7 viral oncoproteins, resulting in a degradation of p53 and functional inactivation of Rb [10].…”

LncRNA AWPPH as a prognostic predictor in human cancers in Chinese population: evidence from meta-analysis

“…Cancer is a major public health problem worldwide, and it has been the leading cause of death in China since 2010 [ 1 ]. Cancer is a highly complex disease involving numerous molecular changes, including chromosomal translocations, deletions and amplification, epigenetic alterations and genetic mutations [ 2–4 ], which make it more difficult to be cured than ordinary diseases. Although great advances have been achieved in diagnoses and treatments, the clinical prognosis remains undesirable in most cancer patients.…”