HPV infection profile in cervical lesions

González-Yebra, Beatriz; Mojica-Larrea, Miriam; Alonso, R.; González, Ana Laura; Romero-Morelos, Pablo; Taniguchi‐Ponciano, Keiko; Ruiz-Romero, Jorge A.; López-Romero, Ricardo; Salcedo, Mauricio

doi:10.24875/gmm.m22000679

Cited by 5 publications

(7 citation statements)

References 15 publications

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“…This milestone approach is primarily dependent on the availability of nationwide HPV genotype data for countries, like Ethiopia. Our finding together with other previous HPV genotype reports could be used to partly evaluate the impact of vaccination and in the future the impact of the screening program in Ethiopia [62].…”

Section: Discussionsupporting

confidence: 52%

“…The reason behind the low involvement of HPV18 in cancer and high-grade cervical lesions in Ethiopia requires further studies. Generally, the difference in the proportion and type of HR-HPVs among different studies in Ethiopia and other parts of the world might vary partly due to studyspecific characteristics, heterogeneity in geographical location [62], age, life style and socio-economic status of the study participants and differences in study population, and most importantly methods used for HPV detection [49,53].…”

Section: Discussionmentioning

confidence: 99%

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High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

Derbie

Maier

Amare

et al. 2023

Infect Agents Cancer

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Background Human papillomavirus (HPV) genotypes differ by geographic location. With the advent of HPV vaccination and HPV-based cervical screening tests in Ethiopia, a nationwide dataset on the genotype distribution of HPV among women has paramount importance in the fight against cervical cancer. However, there is limited data in this regard in the northwest part of the country. Therefore, this study aimed to identify the genotype distribution of high-risk HPVs among women presenting with cervical abnormalities. Methods A health facility-based cross-sectional study was conducted at Felege Hiwot Comprehensive Specialized Hospital (FHCSH), Bahir Dar–Ethiopia. Women aged ≥ 30 years who visited the hospital gynecology unit from 01 March 2019 to 30 October 2021 were included. Following general and pelvic examinations, a senior gynecologist collected cervical punch biopsies for histopathological examinations and cervical swabs for HR-HPV detection using the Abbott Alinity m system (Abbott Molecular, Des Plaines, IL, USA). Extended genotyping was carried out with the INNO-LiPA HPV Genotyping Extra II assay (INNO-LiPA; Fujirebio Europe, Ghent, Belgium) as per the manufacturer protocols at the Institute of Virology, Leipzig University Hospital, Germany. Results We included 355 women with a mean age of 46.4 ± 11.4 years. The majority of the participants, 277 (79.4%) were sexually active before the age of 18 years and 180 (51.6%) had multiple sexual partners. Forty-eight (13.5%) of the participants were HIV positive. The proportion of HR-HPV was 53.0% (n = 188; 95%CI: 47.8–58.1%). From these samples, 13 different HR-HPV types with a total of 258 sequences were identified. The detection of HR-HPV increased significantly with an increase in the age of the participants. The predominant identified HR-HPV was HPV16, 50.4% followed by HPV31 (9.7%), HPV33 (8.5%), HPV39, and HPV68 each (5.8%) and HPV18 (4.7%). Of the total HR-HPV-positive women, 23.9% (45/188) were infected with multiple HR-HPV types. All HPV16, HPV18, HPV35, and HPV45 genotypes (as a single or in coinfections) were found to be associated with either high-grade lesions or cervical cancer. Conclusions HR-HPV infection was reportedly higher among women in the present study area. Based on our findings, we strongly recommend the nonavalent HPV vaccine for immunization and any HPV-based screening method to take into consideration the predominant genotypes circulating in the country. The role of multiple HPV infections in high-grade cervical lesions entails further study in Ethiopia.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

Derbie

Maier

Amare

et al. 2023

Infect Agents Cancer

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“…Since these genotypes belong to the α-6 and α-9 species, this could suggest that phylogenetically related HR-HPV genotypes tend to cluster together, in agreement with reports by other authors. [41][42][43] Use of other contraceptive methods: coitus interruptus, intrauterine device, bilateral tubal occlusion, vasectomy, subdermal implant, or not using any. Herpes simplex virus type 2 test was performed in a StepOne Real-Time PCR system, using the SYBR select master mix.…”

Section: Discussionmentioning

confidence: 99%

“…HPV DNA detection and virus load quantification were performed using the Seegene Anyplex™ II HPV 28 system (Seegene, Seoul, Korea), based on a real-time multiplex PCR assay that allows simultaneous amplification, detection, and differentiation of target nucleic acids of 19 high-risk HPV types (16,18,26,31,33,35,35,39,45,45,51,52,53,56,58,59, 66, 68, 69, 73, 82) and 9 low-risk HPV types (6,11,40,42,43,44,54,61,70). 24 This system allowed us to confirm the HPV diagnosis and estimate viral loads by genotype in each patient.…”

Section: Hpv Genotyping and Pathological Examinationmentioning

confidence: 99%

Prevalence and Risk Factors for High-Risk Human Papillomavirus Infection and Cervical Disorders: Baseline Findings From an Human Papillomavirus Cohort Study

Saldaña-Rodríguez,

Bahena-Román,

Delgado-Romero

et al. 2023

Cancer Control

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Background A persistent infection by high-risk human papillomavirus (HR-HPV) is a prerequisite for the development of cervical neoplasms; however, most studies have focused on risk factors associated with HPV-16 and HPV-18 only. Objectives We assessed the association of risk factors with the prevalence of HPV-16, HPV-18, and non-16/18 HR-HPV infection and with the occurrence of cervical lesions in the baseline of a cohort study of HPV persistence in a Mexican population. Methods Cross-sectional study within the baseline of a 5-year dynamic cohort study of HR-HPV persistence in women with an abnormal cytology study result from 2015 to 2021. HPV DNA was detected using the Anyplex II HPV 28 kit. Data on lifestyle, sociodemographic, and reproductive factors were assessed using bivariate and multivariate analyses to determine the association of risk factors with HR-HPV infection status and histopathologic diagnosis. Results A total of 373 women were included in the study. The overall prevalence of HR-HPV infection was 69.97%. The most prevalent HR-HPV genotypes, including single and multiple infections, were HPV-53 (13.4%), HPV-16 (11.8%), HPV-58 (10.9%), HPV-31 (10.9%), and HPV-66 (10.7%). We found 90 multiple HR-HPV infection patterns, all of them with α-6 and -9 species. Significant associations of multiple HPV-16 and non-16/18 HR-HPV infections were found with marital status, number of lifetime sexual partners, and smoking history. The most prevalent genotype in CIN1 and CIN2 patients was HPV-16. No association was found between biological plausibility risk factors and cervical lesions. Conclusions The risk factors for non-16/18 HR-HPV multiple infections are no different than those linked to HPV-16 multiple infections.

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“…HPV infection is also associated with anal, head and neck cancers, and some cancers of the vulva, penis, and anus [ 5 , 6 , 7 ]. HPV 6 and 11 are related to recurrent respiratory papillomatosis in children, as well as approximately 90% of genital warts [ 8 ]. Studies conducted in recent decades have shown that it is capable of infecting and replicating in syncytiotrophoblast cells, causing adverse events in pregnancy (miscarriage, preeclampsia and preterm birth) [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Correlation of the HPV 16 Genotype Persistence in Women Undergoing LEEP for CIN3 with the Risk of CIN2+ Relapses in the First 18 Months of Follow-Up: A Multicenter Retrospective Study

Bruno,

Valenti,

Ruggeri

et al. 2024

Diagnostics

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Objective: Specific hr-HPV genotypes have different natural histories and different oncogenic capacity. This study aimed to investigate the risk of CIN2+ recurrence of the individual genotypes and evaluate how the duration of HPV persistence influences the risk of developing recurrent 16 cervical dysplasia of high grade (CIN2+). Methods: Data from patients with persistent HPV infection after primary conization were retrospectively extracted. Kaplan-Meier proportional hazards models were used to evaluate associations between the duration of HPV persistence and the risk of developing recurrent CIN2+. Kruskal-Wallis testing with Dunn’s multiple comparison test was used to test whether there was a statistically significant difference in the time to development of tumor recurrences between different genotypes. Results: Overall, 333 patients met the inclusion criteria. In 285 cases the HPV infection was persistent, in 48 cases (18%) it was transient, i.e., different genotypes after LEEP. Overall were diagnosed 39 relapses (13.7%), 79.5% (31/39 cases) were due to genotype 16, 20.5% (8/39) were linked to the other genotypes. Persistence of genotype 16 showed a 7-fold increased risk of developing a CIN2+ relapse, OR = 7.08 (95%CI: 3.12–16.08). Furthermore, the majority of relapses (38/39) occurred within 24 months of persistence with a cut-off represented by 18 months (p = 0.001) in which the relapse rate is maximum and the most frequently found genotype was the 16th with 31 (79.5%) cases of recurrence. Kruskal-Wallis test with Dunn’s multiple comparisons has shown statistically significant difference in the time of development of CIN2 relapses among HPV16 and other genotypes. (p < 0.05). Kaplan-meier analysis has shown statistically significant difference between the time to CIN2+ relapse onset in patients with HPV 16 infection and patients with other hrHPV genotypes. (p < 0.05) Conclusions: the study results suggest that persistent HPV infection after LEEP with the same HR genotype present before surgery represents one of the most important predictive factors of the risk of CIN2+ recurrence. The persistence of HPV16 for the first 18 months strongly correlates with the risk of developing a CIN2+ recurrence.

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HPV infection profile in cervical lesions

Cited by 5 publications

References 15 publications

High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

Prevalence and Risk Factors for High-Risk Human Papillomavirus Infection and Cervical Disorders: Baseline Findings From an Human Papillomavirus Cohort Study

Correlation of the HPV 16 Genotype Persistence in Women Undergoing LEEP for CIN3 with the Risk of CIN2+ Relapses in the First 18 Months of Follow-Up: A Multicenter Retrospective Study

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