HPV infection and p16 promoter methylation as predictors of ASC‐US/LSIL progression

Lee, Hee Yun; Lee, Eun‐Ju

doi:10.1002/cncy.21615

Cited by 15 publications

(12 citation statements)

References 33 publications

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“…Comparing the prevalent histopathological result in ASC cytologies, ASC-US was significantly associated with LSIL or better outcome, whereas ASC-H was more associated with HSIL or worst outcome, in agreement to other authors [4, 11, 40, 41]. In the present study, 13.33% of ASC-US and 64.71% of ASC-H biopsies resulted in HSIL or higher, quite significant numbers indicating a need for follow-up.…”

Section: Discussionsupporting

confidence: 81%

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Atypical Squamous Cells: Cytopathological Findings and Correlation with HPV Genotype and Histopathology

Oliveira

Eleutério

et al. 2018

Acta Cytologica

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Objective: We aimed to assess potential associations between atypical squamous cell (ASC) subgroups: ASC-US (undetermined significance) and ASC-H (cannot exclude high-grade squamous intraepithelial lesion), regarding cytomorphological features, high-risk (HR) human papillomavirus (HPV) testing, and histological outcomes in a sample of Brazilian women. Study Design: Cross-sectional study which evaluated 1,346 liquid-based cytologies between January 2010 and July 2016 with ASC results. ASC-US and ASC-H were analyzed for frequency, diagnostic criteria, and cytological findings and compared with HR-HPV tests and histological outcomes. Results: Enlarged nucleus was the most frequent ASC-US criterion, but alternative criteria were present in 20% of the total cases. No ASC-US criteria were associated with histological outcomes or HR-HPV positivity. Parakeratosis, corneal pearl, giant cells, and binucleation were strongly associated with ASC-US while hyperkeratosis was associated with high-grade squamous intraepithelial lesions (HSIL) or a superior outcome. HR-HPV was positive in 64.39% of ASC-US and 65.38% of ASC-H. HSIL or superior outcomes also occurred in 13.33% of ASC-US and 64.71% of ASC-H cases. Conclusion: Alternative criteria for ASC-US were relatively frequent. Reactive cellular changes suggestive of atypias were more abundant in ASC-US. Although ASC-H is associated with worse histological outcomes, no differences in HPV positivity were found in comparison to ASC-US.

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Section: Discussionsupporting

confidence: 81%

“…These results rarely cause concerns that more significant lesions may be present since 1/3 to 2/3 are HPV negative [10, 11]. There is important regional variation not only in the proportion of cytological atypias that are positive for HPV but also in the worldwide distribution of high-risk HPV [12].…”

Section: Introductionmentioning

confidence: 99%

Atypical Squamous Cells: Cytopathological Findings and Correlation with HPV Genotype and Histopathology

Oliveira

Eleutério

et al. 2018

Acta Cytologica

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“…In this meta-analysis, on the basis of data from over 3000 subjects, we found that the hypermethylation rates in LSIL, HSIL and CC specimens were gradually increased, resulting in a growing trend in effects of P16 INK4a hypermethylation on susceptibility to LSIL, HSIL and CC. These results, combined with the previous epidemiological evidence that P16 INK4a hypermethylation was correlated with the progression of LSIL to HSIL [39, 42], suggest that P16 INK4a promoter hypermethylation may be an epigenetic marker for the progression of cervical carcinogenesis. Hence, detecting P16 INK4a hypermethylation may help clinicians to determine whether patients with cervical neoplasia are in disease regression, persistence or progression.…”

Section: Discussionsupporting

confidence: 70%

Associations of P16INK4a promoter hypermethylation with squamous intra-epithelial lesion, cervical cancer and their clinicopathological features: a meta-analysis

et al. 2016

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“…As large-scale clinical studies have confirmed that the HPV-based screening method possesses a high sensitivity and is easy to repeat, this method is currently recognized as the gold standard for clinical HPV testing. However, it has low specificity [19,20]. Therefore, how to improve the specificity of HPV detection in screening for cervical cancer and avoid over-treatment of patients with positive HPV infection have become major topics of interest.…”

Section: Discussionmentioning

confidence: 99%

Clinical Value of Human Papillomavirus E6/E7 mRNA Detection in Screening for Cervical Cancer in Women Positive for Human Papillomavirus DNA or

Han¹,

Husaiyin²,

Zhao³

et al. 2018

Clin. Lab.

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Background: The detection of human papillomavirus (HPV) E6/E7 mRNA indicates a risk of further deterioration in cervical lesions. We explored the clinical value of HPV E6/E7 mRNA detection in cervical cancer screening in women positive for HPV or with abnormal thin-prep cytology test (TCT) results in the Xinjiang region of China. Methods: A total of 6,800 women were screened in our hospital for cervical cancer by both TCT and HPV DNA testing from August 2013 to June 2015. Of these, 197 had abnormal cytological or HPV test results and subsequently underwent HPV E6/E7 mRNA detection and histopathological examination, while 101 underwent an HPV DNA typing test. Using pathological results as the gold standard, we compared the accuracies of HPV E6/E7 mRNA detection or HPV DNA type testing alone, in parallel, and in series for diagnosing high-grade cervical lesions. Results: Pathological examination revealed 80 cases of chronic cervicitis, 16 cases of cervical intraepithelial neoplasia (CIN)-I, 50 cases of CIN-II-III, and 51 cases of cervical cancer. The area under the receiver operating characteristic (ROC) curve (AUC) for diagnosing high-grade cervical lesions by HPV E6/E7 mRNA detection was 74.95% (sensitivity, 85.15%; specificity, 66.67%; Youden index, 0.139; positive predictive value, 72.9%; negative predictive value, 81.0%; positive likelihood ratio, 2.555; negative likelihood ratio, 0.222; and post-test probability, 72.9%). Conclusions: HPV E6/E7 mRNA detection is superior to HPV DNA type testing for diagnosing high-grade cervical lesions. (Clin. Lab. 2018;64:xx-xx.

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HPV infection and p16 promoter methylation as predictors of ASC‐US/LSIL progression

Cited by 15 publications

References 33 publications

Atypical Squamous Cells: Cytopathological Findings and Correlation with HPV Genotype and Histopathology

Atypical Squamous Cells: Cytopathological Findings and Correlation with HPV Genotype and Histopathology

Associations of P16INK4a promoter hypermethylation with squamous intra-epithelial lesion, cervical cancer and their clinicopathological features: a meta-analysis

Clinical Value of Human Papillomavirus E6/E7 mRNA Detection in Screening for Cervical Cancer in Women Positive for Human Papillomavirus DNA or

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