HPV Genotyping by Molecular Mapping of Tissue Samples in Vaginal Intraepithelial Neoplasia (VaIN) and Vaginal Squamous Cell Carcinoma (VaSCC)

Zhang, Shitai; Saito, Mayumi; Okayama, Kaori; Okodo, Mitsuaki; Kurose, Nozomu; Sakamoto, Jinichi; Sasagawa, Toshiyuki

doi:10.3390/cancers13133260

Cited by 11 publications

(11 citation statements)

References 33 publications

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“…A previous study from our group showed that at least one HR-HPV or pHR-HPV can be detected in CIN2 or CIN3 FFPE tissues, 37 which is consistent with the present results (HPV-positive rate was 100% in CIN2 tissues). One case (case no.…”

Section: Discussionsupporting

confidence: 93%

Prediction value with a novel and accurate tissue‐based human papillomavirus detection method in low‐grade squamous intraepithelial lesions

Wang

Han

et al. 2022

Cancer Medicine

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Background The progression rate from CIN1 to CIN3 is 9.0% and that for invasive cancer is 1.0%. The large majority of CIN1 lesions regress spontaneously, and the treatment of CIN1 is still controversial. Aims The aim of this study is to investigate the responsible HPV genotype in the low‐grade SILs, then to predict the presence of high‐grade SILs, and determine whether further treatment is needed. Methods We use the methods of manual microdissection with FFPE tissue specimens and the E6/E7 uniplex polymerase chain reaction (PCR) to detect HPV in the lesions. Results The HPV test was performed on 72 biopsy tissue specimens, and 55 (76.4%, 55/72) of them were HPV positive. Nine (16.4%, 9/55) of them escalated to CIN2 after LEEP or cervical conization, and 46 (83.6%, 46/55) were still CIN1. There were 17 (23.6%, 17/72) cases with HPV‐negative results in cervical biopsy tissues. HPV test of cervical biopsy diagnosed with CIN1 has a positive predictive value of 16.4% in the presence of CIN2 or higher lesions, a negative predictive value of 94.1%, a specificity of 25.8%, and a sensitivity of 90.0%. HPV test of cervical biopsy tissues for the prediction of HPV infection in LEEP or cone surgery tissues had a positive predictive value of 80.0%, a negative predictive value of 82.3%, a specificity of 56.0%, and a sensitivity of 93.6%. Conclusions It is the first time that we have detected HPV genotype in the low‐grade SILs by the methods of manual microdissection with FFPE tissue specimens and the E6/E7 uniplex PCR. Patients with cervical biopsy tissue diagnosed with CIN1 and with a negative or only low‐risk HPV type result can be considered for follow‐up. Conversely, in cases of cervical biopsy tissue diagnosed with CIN1 positive for high‐risk HPV, surgery or a close follow‐up program can be selected.

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Section: Discussionsupporting

confidence: 93%

Prediction value with a novel and accurate tissue‐based human papillomavirus detection method in low‐grade squamous intraepithelial lesions

Wang

Han

et al. 2022

Cancer Medicine

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“…As especially HPV 16 and HPV 31 were found to be highly associated with high-grade VaIN, it is advisable to be particularly attentive for signs of VaIN among patients tested positive for these hrHPV subtypes [ 53 ]. However, to add another degree of complexity, recent data suggests that concomitant CIN and VaIN in the same patient develop independently, since in the great majority of cases different hrHPV types were identified within the cervical and vaginal lesions [ 54 ]. Both patients who were diagnosed with VaIN 3 in our study were HPV 16 positive and did not have any cervical abnormalities.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of higher-grade dysplasia in persistently high-risk human papillomavirus positive, cytology negative women after introduction of the new cervical cancer screening in Germany

Berger

Wolf-Breitinger

Weiß

et al. 2023

Cancer Causes Control

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Purpose According to the recently implemented organized cervical cancer screening program in Germany, women older than 35 years with negative cytology but persistent high-risk human papilloma virus (hrHPV) infection > 12 months should be referred to colposcopy for further evaluation. This study aimed to present and dissect colposcopic and histopathological findings with particular focus on associated hrHPV genotypes. Methods This study is a retrospective analysis of clinical data from 89 hrHPV positive patients with normal cytology who underwent colposcopic examination at a certified dysplasia outpatient clinic in Germany in 2021. Results While 38 (43%) women had a normal colposcopic finding, 45 (51%) had minor and 6 (7%) major changes. Thirty-one (35%) of the women were HPV 16 and/or HPV 18 positive and 58 (65%) women were positive for other hrHPV only. Among patients who underwent colposcopy with biopsies (in case of an abnormal finding or type 3 transformation zone, n = 68), eight (12%) had cervical intraepithelial neoplasia (CIN) 3 and six (9%) had CIN 2. The proportion of women diagnosed with CIN 3 varied among different hrHPV genotypes (HPV 16: 11%, HPV 18: 33%, HPV 31: 27%, HPV 33: 33%, HPV 52: 33%). Conclusion Persistently hrHPV positive women with negative cytology are at increased risk of being diagnosed with CIN 3. As CIN 3 prevalence seems to differ with regard to hrHPV strain, immediate HPV genotyping for risk stratification and subsequent early referral for colposcopy might constitute a feasible strategy.

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“…This may be due to the prevalence of HPV58 in patients with CIN. In most instances, the differing distribution of HPV types between the vagina and cervix suggests that VaIN and CIN develop independently of each other [22]. The results indicated the close association of high-risk HPV infection with the occurrence of concurrent VaIN and CIN.…”

Section: Prevalence and Clinical Data Distributionmentioning

confidence: 85%

A single-institutional retrospective analysis of related factors for vaginal intraepithelial neoplasia

Hongmin,

Qianling,

Dan

2023

Preprint

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Background To date, there are only a few studies on the related factors of vaginal intraepithelial neoplasia (VaIN). This study analyzes the features of VaIN and identify underlying risk factors. Methods Patients with histologically confirmed VaIN at the Industrial Street Branch of Chengdu Women’s and Children’s Central Hospital from July 2020 to December 2021 were objectively identified. We used the baseline clinical characteristics, human papillomavirus (HPV), cytology, and pathology of the cases enrolled for statistical analysis. Results A total of 62 patients with VaIN (mean age: 39.06 ± 11.66 years; range: 19–69 years) were included, with 16 patients having cervical intraepithelial neoplasia (CIN) complications. Synchronous CIN was identified in 46 (74.2%) patients. Except for one patient with vulvar itching, almost all patients were asymptomatic. The most common cytological abnormalities among women were atypical squamous cells of undetermined significance (ASC-US) (59.5%) and low-grade squamous intraepithelial lesions (LSIL) (21.6%). Abnormalities occurred in six (37.5%) out of 16 patients with VaIN only and in 31 (67.4%) out of 46 patients with concurrent CIN (P = 0.0358). Additionally, 60 (96.8%) patients developed HPV infection. The most prevalent HPV genotypes were HPV16 in patients with VaIN alone and both HPV58 and HPV16 in patients with concurrent CIN. Conclusions Attention should be paid to HPV16- and HPV58-positive patients with cytological abnormalities such as ASC-US and LSIL (especially concurrent CIN) to avoid misdiagnosis or underdiagnosis and to facilitate early interventions for VaIN.

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HPV Genotyping by Molecular Mapping of Tissue Samples in Vaginal Intraepithelial Neoplasia (VaIN) and Vaginal Squamous Cell Carcinoma (VaSCC)

Cited by 11 publications

References 33 publications

Prediction value with a novel and accurate tissue‐based human papillomavirus detection method in low‐grade squamous intraepithelial lesions

Prediction value with a novel and accurate tissue‐based human papillomavirus detection method in low‐grade squamous intraepithelial lesions

Prevalence of higher-grade dysplasia in persistently high-risk human papillomavirus positive, cytology negative women after introduction of the new cervical cancer screening in Germany

A single-institutional retrospective analysis of related factors for vaginal intraepithelial neoplasia

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