HPV genotype analysis for women in Shaanxi Province of China

Abstract

ABSTRACT. The aim of this study was to examine the subtype distribution of human papilloma virus (HPV) in women in the Shaanxi Province of China. A DNA chip, along with polymerase chain reaction amplification and reverse dot blot technology, was adopted to analyze the HPV genotypes of 22,937 cases of cervical cell specimens. The HPV infection rate was 18.70%, wherein high-risk, low-risk, and high-and low-risk multiple infection rates were 15.75, 2.96 and 1.91%, respectively. High-risk infections accounted for 84.20% of total infections. The rate of HPV infection in women with rural residence, high school education or less, a low income, or age over 40 years was significantly higher than that in the control group (negative HPV infection women). Of the 18 detected high-risk HPV subtypes, the most common in single infections were, in the order of prevalence, HPV16, 58, 18, 52, 33, and 56. For multiple high-risk infections, the most common subtypes in the order of prevalence were HPV16, 52, 58, 18, 56, and 33. Age was a factor in the rate of infection, as the 41- 50-year age group had a significantly higher risk of infection than the other groups (P < 0.05). In multiple infections, double infections were common, accounting for 77.10% of multiple infections, and triple or more infections were more common in women aged 51-60 years. In Shaanxi Province, high-risk HPV infection in women was mainly attributed to rural residence, age over 40 years, low income, and low education level.

The Characteristics of Human Papillomavirus Infection in Women with Vaginitis and the Correlation with Vaginitis

“…HPV subtypes examined in our study, HPV58, 52, and 16 had the highest prevalence in overall, single-type and multiple-type infected participants, which is slightly different from some data for screening [17]. The study also showed that the most common infection was with HPV58, which was detected in 10.34% of the women with vaginitis.…”

“…To further investigate the relationship between vaginitis and HPV infection, 447 patients with vaginitis were chosen for HPV genotyping results, showing that the total HPV infection rate was 39.60% in women with vaginitis. Previous studies have focused on the prevalence of HPV infection in different countries [16,17] and within China, and the prevalence varies from 9.9 to 31.9% in different areas in China [18][19][20].In our study, the infection rate was 39.60%, indicating a higher frequency in women with vaginitis. Considering this high prevalence, it is indispensable to know the HPV genotype distribution for vaccine development in women with vaginitis.…”

Prevalence and genotype distribution of human papillomavirus among women with cervical lesions in Shenzhen city, China

“…In our study, the overall HPV prevalence rate was 17.83% (937/5,255) in Shenzhen city. However, HPV infection rates are known to vary across different regions in China (7.3% in Guangdong, 15 9.9% in Beijing, 16 10.3% in Liaoning, 17 18.7% in Shaanxi, 18 and 24.7% in Xinjiang 19 ), and across different countries (8.0% to 27.3%). 9,[20][21][22] Demographic factors such as ethnicity, socioeconomic status, health conditions, and education level may contribute to the uneven distribution of HPV incidence.…”

“…The 21 HPV GenoArray Diagnostic Kit is a qualitative polymerase chain reaction (PCR)-based test used for the detection and determination of 21 specific HPV DNAs in cervical specimens. The 21 HPV genotypes are divided into 15 high-risk genotypes (HPV 16,18,31,33,35,39,45,51,52,53,56,58,59, 66 and 68) and 6 low-risk genotypes (HPV 6,11,42,43,44,CP8304), and the specific detection steps were followed in strict adherence to instructions, as described previously. 13,14 Briefly, the HPV DNA extracted from cervical samples was amplified using PCR, and then hybridized with specific HPV probes located inside the membrane strip, followed by the use of the enzyme immunoassay method to obtain colorimetric results.…”