HPV Detection in Head and Neck Squamous Cell Carcinomas: What Is the Issue?

Lépine, Charles; Morini, Aurélien; Brunet, Anaïs; Veyer, David; Brochard, Camille; Mirghani, Haïtham; Péré, Hélène; Badoual, Cécile

doi:10.3389/fonc.2020.01751

Cited by 52 publications

(64 citation statements)

References 136 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Though the current standard methods for detection of HPV infection in OPSCC are based on detection of E6/E7 mRNA detection [ 33 ], OPSCC tumor samples were mainly obtained as FFPE tissue in which RNA might not be reliably preserved [ 34 – 36 ]. Therefore, in our study, we selected HPV DNA detection method by qPCR to confirm the presence of HPV infection in HNSCC.…”

Section: Discussionmentioning

confidence: 99%

Discrepancy in p16 expression in patients with HPV-associated head and neck squamous cell carcinoma in Thailand: clinical characteristics and survival outcomes

et al. 2021

View full text Add to dashboard Cite

Background Lower prevalence HPV infection has been previously reported in Thai population when compared with Western countries. p16 expression indicates HPV-associated oropharyngeal squamous cell carcinoma (OPSCC), but not non-OPSCC. We therefore evaluated the characteristic and association of p16 and HPV in Thai patients with HNSCC. Methods We used immunohistochemistry and qPCR, respectively, to detect p16 and HPV DNA in archrival formalin-fixed paraffin-embedded HNSCC tissues. Patient characteristics and survival were analyzed. Results p16 expression was detected in tumors of 72 of 662 (10.9%) patients with HNSCC and was significantly associated with higher-grade histology, advanced nodal stage, and oropharynx. p16 was expressed in 28 and 6.5% of patients with OPSCC or non-OPSCC, respectively, and HPV DNA was detected in 15.6 and 1% of patients, respectively. Using p16 as a surrogate for HPV status, sensitivities were 80 and 25% in OPSCC and non-OPSCC, respectively. Positive and negative predictive rates of OPSCC were 38 and 95%. Discordance rates between HPV and p16 were 23 and 7% in OPSCC and non-OPSCC, respectively. Overall survival (OS) were significantly longer in both p16-positive OPSCC (p = 0.049), and non-OPSCC (p = 0.003). Conclusions Low prevalence of p16 and HPV associated OPSCC and non-OPSCC were confirmed in Thai patients. High discordance and low positive predictive rates of p16 were observed in HPV-associated OPSCC. p16 was a significant prognostic factor for OS for patients with OPSCC or non-OPSCC. Therefore, HPV testing should be performed to assess the association of HPV with HNSCC regardless of p16 expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Discrepancy in p16 expression in patients with HPV-associated head and neck squamous cell carcinoma in Thailand: clinical characteristics and survival outcomes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The fluorescent signal is read in real time at each amplification cycle, generating curves which, compared to a standard, allow for a quantitative evaluation of the viral load. Several studies have shown a statistically valid correlation between viral load and risk of developing cervical cancer and high sensitivity, reproducibility, and speed of execution [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…The determination of the mRNA transcripts of E6/E7 oncoproteins, a useful prognostic indicator of the progression of lesions towards cervicocarcinoma, uses NASBA (nucleic acid sequence-based amplification) technology based on isothermal amplification (conducted at 41°C) of the target RNA and on the use of particular probes defined as molecular beacons capable of emitting fluorescence at two different wavelengths when a complementary sequence is present [ 35 , 45 – 47 ].…”

Section: Resultsmentioning

confidence: 99%

Human Papilloma Virus: Current Knowledge and Focus on Oral Health

Fiorillo

Cervino

Surace³

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

The human papilloma virus (HPV) is responsible for different pathological manifestations in humans. This agent gives rise to lesions of different types and in different areas of the organism, including the oral cavity. The aim of this study is to show which are the main diseases for which HPV is responsible and to bring to light some of the interceptive and therapeutic strategies. The analysis was conducted by consulting the major scientific databases with the aim of obtaining information on the characteristics of oral HPV and its management; furthermore, the literature was supported by some clinical cases proposed by the authors. The role of dentistry is essential in the early diagnosis of this type of pathologies and above all in knowing how to direct patients towards a path that can lead to patient management, especially in the event that these lesions have a malignant potential. Enhancing the knowledge and role of dentistry can lead to early diagnosis of this type of injury, intercepting a pathology that could have multiorgan implications.

show abstract

“…Some risk factors have been described, including smoking, alcohol abuse, and human papillomavirus (HPV) infection [ 1 , 2 ]. HPV plays a role in the development of a subset of HNCs and, notably, oropharyngeal cancer (OPC) [ 3 ]. The role of HPV is not so clear in non-oropharyngeal carcinomas (non-OPCs), but some reports suggest a possible association between HPV infection and nasopharyngeal cancers [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…HPV plays a role in the development of a subset of HNCs and, notably, oropharyngeal cancer (OPC) [ 3 ]. The role of HPV is not so clear in non-oropharyngeal carcinomas (non-OPCs), but some reports suggest a possible association between HPV infection and nasopharyngeal cancers [ 3 , 4 , 5 ]. HPV-induced OPC has different biological behavior and a better prognosis compared to non-HPV-induced OPC, and the eighth-edition Tumor-Node-Metastasis (TNM) classification now separates these two types [ 3 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Nutritional Support in Head and Neck Radiotherapy Patients Considering HPV Status

et al. 2020

View full text Add to dashboard Cite

Malnutrition is a common problem in patients with head and neck cancer (HNC), including oropharyngeal cancer (OPC). It is caused by insufficient food intake due to dysphagia, odynophagia, and a lack of appetite caused by the tumor. It is also secondary to the oncological treatment of the basic disease, such as radiotherapy (RT) and chemoradiotherapy (CRT), as a consequence of mucositis with the dry mouth, loss of taste, and dysphagia. The severe dysphagia leads to a definitive total impossibility of eating through the mouth in 20–30% of patients. These patients usually require enteral nutritional support. Feeding tubes are a commonly used nutritional intervention during radiotherapy, most frequently percutaneous gastrostomy tube. Recently, a novel HPV-related type of OPC has been described. Patients with HPV-associated OPC are different from the HPV− ones. Typical HPV− OPC is associated with smoking and alcohol abuse. Patients with HPV+ OPC are younger and healthy (without comorbidities) at diagnosis compared to HPV− ones. Patients with OPC are at high nutritional risk, and therefore, they require nutritional support in order to improve the treatment results and quality of life. Some authors noted the high incidence of critical weight loss (CWL) in patients with HPV-related OPC. Other authors have observed the increased acute toxicities during oncological treatment in HPV+ OPC patients compared to HPV− ones. The aim of this paper is to review and discuss the indications for nutritional support and the kinds of nutrition, including immunonutrition (IN), in HNC, particularly OPC patients, undergoing RT/CRT, considering HPV status.

show abstract

HPV Detection in Head and Neck Squamous Cell Carcinomas: What Is the Issue?

Cited by 52 publications

References 136 publications

Discrepancy in p16 expression in patients with HPV-associated head and neck squamous cell carcinoma in Thailand: clinical characteristics and survival outcomes

Discrepancy in p16 expression in patients with HPV-associated head and neck squamous cell carcinoma in Thailand: clinical characteristics and survival outcomes

Human Papilloma Virus: Current Knowledge and Focus on Oral Health

Nutritional Support in Head and Neck Radiotherapy Patients Considering HPV Status

Contact Info

Product

Resources

About