HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

Abstract

The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa. From the HPTLC fingerprint the florescent band (under 366 nm) at R f : 0.56 (mobile phase chloroform : toluene : ethyl acetate (6 : 3 : 1, v/v)) was found in leaf, root, and stem of R. tuberosa. So, the florescent band (under 366 nm) at R f : 0.56 was isolated as marker compound RT-F2 from root of R. tuberosa. The marker compound RT-F2 was quantified by using HPTLC technique. The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively. Further study is suggested to characterization and biological nature of marker compound.

HPTLCFinger Printing Profile Of Drynaria Quercifolia(L.) J. Sm. With Biomarker Naringin

“…HPTLC fingerprinting profile with biomarker is an important tool for the identification and the standardization of medicinal plants. [10] Owing to its high separation power, performance and reproducibility, it is considered superior to classical TLC. Naringin is a flavanone glycoside formed from the flavanone naringenin and the disaccharide neohesperidose, [11] is identified as a marker phytochemical in the plant Drynaria quercifolia (L.) J. Sm.…”

“…[7] So chromatographic fingerprints with biomarker helps in accurate authentication as well as quality of the plants. [8] Even though high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods of extracts from the rhizome of this plant is reported in the literature, there is no HPTLC method of aqueous extract with marker compound naringin. [3], [9] Thus the present study is aimed at development of HPTLC finger printing profile of aqueous extract of rhizome of Drynaria quercifolia (L.) J. Sm.…”

Influence of Extraction Parameters on the Yield, Phytochemical, TLC-Densitometric Quantification of Quercetin, and LC-MS Profile, and how to Standardize Different Batches for Long Term from Ageratum conyoides L. Leaves

“…It is verry difficult to identify correct marker compounds for all traditional medicinal, but using chromatographic fingerprint, the authencation and identification of herbal medicines can be accurately conducted. 25 Therefore, the use of markers and chromatographic fingerprinting techniques can give useful information assisting manufacturing control, minimising variations in production batches and assuring batch to bacth consistency, with reproducible results. 26 As shown in Figure 5, TLC profiles showed that the R f value of all samples extract at 0.51 (M2) equal to the standard quercetin.…”

Elucidation of β-sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Thin Layer Chromatography

“…The analytical technique selected is specific and solution stable, provides linearity of 0.999 and 99.24% of sample. 1,[33][34][35] Thus, the results depicted in TLC and HPTLC experimentation played a vital role in our research helping us to identify the β-sitosterol with their percentage in the plant extracts.…”