HPTLC Fingerprint Authentication of Selected Sideritis spp. Using a Pharmacognostic Approach

Over the last years, Sideritis extracts were shown to improve memory. However, their potential to promote the generation of new neurons, starting with the neuronal differentiation of neural stem cells, remains unexplored. Therefore, the present study aimed to evaluate the neurogenic effects of different Sideritis infusions in neural stem and precursor cells and their impacts on cell viability. Moreover, the metabolic fingerprints were recorded using LC-DAD, LC-ESIHRMS, and GC-MS. The neurogenic potential from infusions of the eight Sideritis taxa tested was as potent as the classical neuronal inducer combination of retinoic acid and valproic acid. Further cytotoxicity assays revealed that the IC50 values of extracts were comprised between 163 µg/mL and 322 µg/mL. Hierarchical cluster analyses of the metabolic fingerprints unveiled, that the two Sideritis taxa with the lowest IC50 values were the most divergent in the analytical techniques used. As the analysis focused on polyphenols, it is reasonable to assume that these compounds are responsible for the effect on the cell viability of SH-SY5Y neuroblastoma cells. This study is the first report on the neurogenic potential of Sideritis taxa and might support the use of Sideritis herbal preparations in the context of neurodegenerative diseases.

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“…4 c ). This is in accordance with previous findings in the specific plants 10 , 28 , 29 , 30 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 .…”

Section: Resultssupporting

confidence: 94%

“…4 c). This is in accordance with previous findings in the specific plants [10,[28][29][30][32][33][34][35][36][37][38][39][40].…”

Section: Resultssupporting

confidence: 94%

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

et al. 2023

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“…Siderol is an ent-kaurane diterpenoid obtained from the hexane extract of cultivated Sideritis scardica (Lamiaceae) [ 25 ]. Sideritis involves around 150 species, of which many are popular herbal medicines in Mediterranean folk medicine [ 26 , 27 ]. “Ironwort” is the common name for the aerial parts of various species of Sideritis plants.…”

Section: Discussionmentioning

confidence: 99%

Siderol Inhibits Proliferation of Glioblastoma Cells and Acts Synergistically with Temozolomide

et al. 2022

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Glioblastoma (GBM) is the most aggressive primary central nervous system (CNS) tumor in adults with dismal prognosis. Currently, the therapeutic interventions include gross total resection, when possible, followed by radiotherapy and chemotherapy. However, despite treatment, tumor usually recurs within 7–9 months. The presence of glioma cells with stem-like properties and tumor’s heterogeneity have been identified as the most important factors driving recurrence. Recently, research efforts have been focused on the use of natural substances as treatment for GBM. Siderol is an ent-kaurane diterpenoid, isolated from the genus Sideritis. Sideritis extracts have already been investigated for their anti-inflammatory, antioxidant, and anticancer effects. In this study, we investigated the antitumoral effects of siderol in GBM T98 and U87 cell lines, as well as the effects of combined treatment with temozolomide (TMZ). Cell viability was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue exclusion assay. Different concentrations of siderol were used in order to calculate the IC50 values at 72 h after treatment. Flow cytometry used for the DNA cell cycle analysis after treatment with siderol in concentrations of IC50 and twice the IC50 values for 72 h. Furthermore, the effect of siderol in cell’s migratory ability was tested using wound healing assay. Cell viability and proliferation, after combined treatment with siderol and TMZ, also were evaluated with the trypan blue exclusion assay and the effects of the combination treatment were analyzed with CompuSyn software. Treatment with siderol significantly reduced cell viability in T98 and U87 cell lines in a dose-dependent manner and IC50 values were calculated, 18 μM and 13 μM, respectively. Moreover, siderol induced G0/G1 cell cycle arrest in a dose-dependent manner and inhibited the migration in both cell lines. In addition, siderol and TMZ seem to have synergistic action in the majority of tested concentrations in both T98 and U87 cells. In conclusion, siderol may represent an innovative strategy for the treatment of GBM, and further studies are needed on siderol’s efficacy and mode of action.

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Herba Sideritis: A putative adaptogen for reducing the risk of age-related cognitive decline and neurodegenerative disorders

Panossian

2024

Phytomedicine Plus

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HPTLC Fingerprint Authentication of Selected Sideritis spp. Using a Pharmacognostic Approach

Cited by 3 publications

References 38 publications

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

Siderol Inhibits Proliferation of Glioblastoma Cells and Acts Synergistically with Temozolomide

Herba Sideritis: A putative adaptogen for reducing the risk of age-related cognitive decline and neurodegenerative disorders

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