“…High performance thin-layer chromatography (HPTLC) platformed detections as a hyphenation technology enabled a new chance for analytical chemistry [5][6][7] , because it provides an excellent tool for screening the analyte in samples that were difficult for traditional methods based on column chromatography [8][9][10][11] . First, this hyphenation technology inherits the essential advantages of HPTLC like high throughput and matrix-tolerance [8,[12][13][14] . A single separation of many samples can be performed simultaneously.…”
Section: Food Science and Human Wellnessmentioning
As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormonelike adverse effects. Herein, a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantifi cation and confi rmation of methyl paraben was proposed and validated in vinegar and coconut juice. First, the detectability of the bioautography to the analyte on different layer materials was estimated, revealing that normal silica gel was the best choice. After that, the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices. The separation result was then coupled to the optimized bioautography, enabling instant and straightforward screening of the targeted compound. For accurate quantifi cation, bioluminescent inhibition pattern caused by the analyte was processed by image analysis, giving useful sensitivity (LOD > 16 mg/kg), precision (RSD < 10.1%) and accuracy (spike-recovery rate 76.9%-112.2%). Finally, the suspected result was confi rmed by determining its MS fi ngerprint, further strengthening the reliability of screening.
“…High performance thin-layer chromatography (HPTLC) platformed detections as a hyphenation technology enabled a new chance for analytical chemistry [5][6][7] , because it provides an excellent tool for screening the analyte in samples that were difficult for traditional methods based on column chromatography [8][9][10][11] . First, this hyphenation technology inherits the essential advantages of HPTLC like high throughput and matrix-tolerance [8,[12][13][14] . A single separation of many samples can be performed simultaneously.…”
Section: Food Science and Human Wellnessmentioning
As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormonelike adverse effects. Herein, a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantifi cation and confi rmation of methyl paraben was proposed and validated in vinegar and coconut juice. First, the detectability of the bioautography to the analyte on different layer materials was estimated, revealing that normal silica gel was the best choice. After that, the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices. The separation result was then coupled to the optimized bioautography, enabling instant and straightforward screening of the targeted compound. For accurate quantifi cation, bioluminescent inhibition pattern caused by the analyte was processed by image analysis, giving useful sensitivity (LOD > 16 mg/kg), precision (RSD < 10.1%) and accuracy (spike-recovery rate 76.9%-112.2%). Finally, the suspected result was confi rmed by determining its MS fi ngerprint, further strengthening the reliability of screening.
“…Apart from that, HPTLC also displayed many intrinsic advantages, such as simplicity, matrix-tolerance and high-throughput. This enabled the analyst to expand the capacity of HPTLC by easily integrating a large array of assays that conventionally performed interpedently, such as surface enhanced Raman spectroscopy [ 15 , 16 , 17 ] and IR [ 18 , 19 ]. Particularly, HPTLC separation in combination with elaborately chosen effect-directed assays, also termed bioautography, had been demonstrated a novel and promising tool for screening of compounds with specific bioactivity in bio-mixtures [ 20 , 21 , 22 , 23 ].…”
Teas based on nutraceutical herbs are an effective tool against hyperlipidemia. However, the adulteration with chemical drugs is frequently detected. By coupling bioluminescent bioautography with high performance thin-layer chromatography (HPTLC), we developed a facile method suitable for screening hypolipidemic drugs (ciprofibrate and bezafibrate) adulteration in five different herbal teas (lotus leaf, Apocynum, Ginkgo biloba, Gynostemia and chrysanthemum). First, the sensitivity of a bioluminescent bacteria to the analyte was evaluated on different HPTLC layer materials, revealing that the best performance was achieved on the silica gel layer. On this basis, sample extracts were separated on silica gel plates via a standardized HPTLC procedure, forming a selective detection window for the targeted compound. Then, the separation results were rapidly visualized by the bioluminescence inhibition of bacteria cells within 6 min after dipping. The observed inhibition displayed an acceptable limit of detection (<20 ng/zone or 2 mg/kg) and linearity (R2 ≥ 0.9279) within a wide concentration range (50–1000 ng/zone). Furthermore, the optimized method was performed with artificially adulterated samples and the recovery rates were determined to be within the range of 71% to 91%, bracing its practical reliability. Showing superiorly high simplicity, throughput and specificity, this work demonstrated that the analytical method jointly based on HPTLC and bioautography was an ideal tool for screening bioactive compounds in complex biological matrix.
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