HPTLC densitometric quantification of stigmasterol and lupeol from Ficus religiosa

Rathee, Deepti; Rathee, Sushila; Rathee, Permender; Deep, Aakash; Anandjiwala, Sheetal; Rathee, Dharmender

doi:10.1016/j.arabjc.2011.01.021

Cited by 31 publications

(12 citation statements)

References 30 publications

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“…It has proved a very useful technique because of its low operating cost, high sample throughput and the need for minimum sample clean-up. The major advantage of HPTLC is in reducing analysis time and cost per analysis [17]. However, no single method was found in the literature to our knowledge to detect stigmasterol in methanolic extract of rhizomes of Alpinia calcarata.…”

Section: Introductionmentioning

confidence: 92%

“…Quantitative determination of stigmasterol and lupeol from Ficus religiosa by HPTLC [17]. HPTLC method which has been developed and validated for quantitation of stigmasterol from petroleum ether extract of dried leaf, stem and flower of Ageratum conyzoides [36].…”

Section: Discussionmentioning

confidence: 99%

“…The final dried samples were stored in labeled sterile bottles and kept at-20 °C. The filtrate obtained was used as sample solution for the further HPTLC analysis [17].…”

Section: Preparation Of Methanol Extractsmentioning

confidence: 99%

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Identification, Quantification and Validation of Stigmasterol From Alpinia Calcarata Using High-Performance Thin Layer Chromatography Method

Pratheema¹,

Cathrine²

2017

Int J Pharm Pharm Sci

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Objective: The present study is designed to develop a new simple, precise, rapid and selective high-performance thin-layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata. Methods:As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml. Results:The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng-250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol. Conclusion:The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

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Section: Introductionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Identification, Quantification and Validation of Stigmasterol From Alpinia Calcarata Using High-Performance Thin Layer Chromatography Method

Pratheema¹,

Cathrine²

2017

Int J Pharm Pharm Sci

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“…Although high performance thin layer chromatography (HPTLC) has a few restrictions, such as a low plate efficiency and narrow developing distance compared with GC and HPLC, it remains a valuable tool for quality assessment of natural products due to its ease, low cost and few requirements, and it has been profitably utilized to develop a chromatographic fingerprint for various natural products, herbal drugs, and commercial herbal formulations. Moreover, the colourful pattern and quantification at the micron and nanogram levels helps to differentiate various samples on the same plate [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of HPTLC method for simultaneous estimation of curcumin and galangin in polyherbal capsule dosage form

Kharat

Namdeo

Mehta

2017

Journal of Taibah University for Science

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Simple and precise HPTLC methods were developed for the simultaneous estimation of two anti-inflammatory drugs (curcumin and galangin). The method was tailored to analyze both drugs in their commercial dosage form (capsules) with no interference from ingredients. Chromatographic separation was performed over precoated TLC plates (60 F 254 , 20 cm × 10 cm, 250 m thickness, Merck, Darmstadt, Germany) via a linear ascending technique using n-hexane, ethyl acetate, acetic acid, and methanol as the mobile phase. Detection and quantification was achieved at 404 nm through spectro-densitometric analysis. Analytical performance of the proposed HPTLC method was validated according to the ICH guidelines with respect to the linearity, accuracy, precision, detection and quantitation limits, robustness and specificity. The calibration curves were linear with the limits of 80-450 and 200-1200 ng/spot for CU and GA, respectively, with correlation coefficients (r 2 ) >0.9998. The limits of detection were 18.31 and 40.50 ng/spot for CU and GA, respectively. The validated HPTLC method was successfully applied to the simultaneous determination of CU and GA in the commercial polyherbal formulation.

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“…HPTLC is widely used as a routine analytical method for the fingerprinting of compounds in complex herbal extracts and quantification of detectable compounds that can be used as DOI: 10.3109/13880209.2015.1066397 markers in quality control of herbal drugs (Rathee et al, 2011). HPTLC analysis of the extract/fractions of L. prostrata confirmed the presence of lupeol and b-sitosterol.…”

Section: Hptlc Analysismentioning

confidence: 99%

Evaluation of antiurolithiatic and antioxidant potential ofLepidagathis prostrata: APashanbhedplant

Devkar

Chaudhary

Adepu

et al. 2015

Pharmaceutical Biology

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Context: Oxidative stress acts as an essential mediator in the pathophysiology of urolithiasis. Lepidagathis prostrata Dalz. (Acanthaceae) is a Pashanbhed plant that is recommended for the management of urolithiasis; however, no scientific validation has been reported. Objectives: To evaluate the antiurolithiatic and antioxidant potential of L. prostrata. Materials and methods: Methanol extract (LPM) and fractions; petroleum ether (LPPE), ethyl acetate (LPEA), n-butanol (LPBU) and aqueous (LPAQ) were prepared. In vitro antiurolithiatic activity was evaluated by the capacity to inhibit calcium oxalate (CaOx) nucleation and aggregation at different concentrations of extract/fractions (0.04-3 mg/mL) for 30 min. Total phenol and flavonoid content and antioxidant potential were determined. A validated HPTLC method was performed to quantify lupeol and b-sitosterol. Results: LPEA exhibited the highest dose-dependent inhibition of CaOx nucleation (IC 50 : 336.23 ± 30.79 mg/mL) and aggregation (IC 50 : 149.63 ± 10.31 mg/mL), which was significantly (p50.05) better than standard Cystone Õ . The polar LPBU fraction was enriched with phenols (47.34 ± 0.19 mg GAE/g) and flavonoids (20.38 ± 0.05 mg QE/g), which correlates with its highest antioxidant potential in DPPH, ABTS, nitric oxide scavenging and iron chelating activities (IC 50 : 1.18-87.34 mg/mL). To our knowledge, this is the first study reporting the presence of lupeol and b-sitosterol in L. prostrata. Conclusion: The antiurolithiatic activity of L. prostrata is probably mediated through the inhibition of CaOx crystallization. In addition to its free radical scavenging and antioxidant activities, it would act as an excellent agent for the prevention of urolithiasis.

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HPTLC densitometric quantification of stigmasterol and lupeol from Ficus religiosa

Cited by 31 publications

References 30 publications

Identification, Quantification and Validation of Stigmasterol From Alpinia Calcarata Using High-Performance Thin Layer Chromatography Method

Identification, Quantification and Validation of Stigmasterol From Alpinia Calcarata Using High-Performance Thin Layer Chromatography Method

Development and validation of HPTLC method for simultaneous estimation of curcumin and galangin in polyherbal capsule dosage form

Evaluation of antiurolithiatic and antioxidant potential ofLepidagathis prostrata: APashanbhedplant

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