HPLC with diode‐array detection for the simultaneous determination of di(2‐ethylhexyl)phthalate and mono(2‐ethylhexyl)phthalate in seminal plasma

Mazzeo, Pietro; Pasquale, Dorina di; Ruggieri, Fabrizio; Fanelli, Maria; D’Archivio, Angelo Antonio; Carlucci, Giuseppe

doi:10.1002/bmc.870

Cited by 20 publications

(9 citation statements)

References 27 publications

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“…Before analysis, known concentration samples were treated by SPE and analysed by RP-HPLC-ESI-MS to test SPE efficiency. In our study the efficiency of SPE procedure is in accordance with the literature (Mazzeo et al, 2007). Treated urine samples could be stored at 4°C without degradation.…”

Section: Methodssupporting

confidence: 85%

Di-(2-Ethylhexyl) Phthalate and Autism Spectrum Disorders

et al. 2012

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ASDs (autism spectrum disorders) are a complex group of neurodevelopment disorders, still poorly understood, steadily rising in frequency and treatment refractory. Extensive research has been so far unable to explain the aetiology of this condition, whereas a growing body of evidence suggests the involvement of environmental factors. Phthalates, given their extensive use and their persistence, are ubiquitous environmental contaminants. They are EDs (endocrine disruptors) suspected to interfere with neurodevelopment. Therefore they represent interesting candidate risk factors for ASD pathogenesis. The aim of this study was to evaluate the levels of the primary and secondary metabolites of DEHP [di-(2-ethylhexyl) phthalate] in children with ASD. A total of 48 children with ASD (male: 36, female: 12; mean age: 11±5 years) and age- and sex-comparable 45 HCs (healthy controls; male: 25, female: 20; mean age: 12±5 years) were enrolled. A diagnostic methodology, based on the determination of urinary concentrations of DEHP metabolites by HPLC-ESI-MS (HPLC electrospray ionization MS), was applied to urine spot samples. MEHP [mono-(2-ethylhexenyl) 1,2-benzenedicarboxylate], 6-OH-MEHP [mono-(2-ethyl-6-hydroxyhexyl) 1,2-benzenedicarboxylate], 5-OH-MEHP [mono-(2-ethyl-5-hydroxyhexyl) 1,2-benzenedicarboxylate] and 5-oxo-MEHP [mono-(2-ethyl-5-oxohexyl) 1,2-benzenedicarboxylate] were measured and compared with unequivocally characterized, pure synthetic compounds (>98%) taken as standard. In ASD patients, significant increase in 5-OH-MEHP (52.1%, median 0.18) and 5-oxo-MEHP (46.0%, median 0.096) urinary concentrations were detected, with a significant positive correlation between 5-OH-MEHP and 5-oxo-MEHP (rs = 0.668, P<0.0001). The fully oxidized form 5-oxo-MEHP showed 91.1% specificity in identifying patients with ASDs. Our findings demonstrate for the first time an association between phthalates exposure and ASDs, thus suggesting a previously unrecognized role for these ubiquitous environmental contaminants in the pathogenesis of autism.

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Section: Methodssupporting

confidence: 85%

Di-(2-Ethylhexyl) Phthalate and Autism Spectrum Disorders

et al. 2012

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“…Until now, the applied preconcentration methods for the determination of MEHP have been limited to the conventional extraction methods such as liquid-liquid extraction (LLE), [49][50][51][52] accelerated solvent extraction (ASE), 53 and solid-phase extraction (SPE). 54 A comparison of the proposed method with reported methods has been listed in Table 3.…”

Section: Methods Comparisonmentioning

confidence: 99%

Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid–liquid microextraction combined with high performance liquid chromatography

2013

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“…Reversed-phase high-performance liquid chromatography (RP-HPLC) is an extensively applied technique in the separation and determination of a wide range of multi-class compounds, including biomolecules, pharmaceuticals, and industrial chemicals, in human, environmental, and food samples [1,2,3,4]. Separation of complex mixtures by RP-HPLC generally requires the application of mobile-phase gradients to overcome the typical disadvantages of isocratic elution, such as poor resolution of early peaks, broadening of late peaks, band tailing, and long separation times [5,6].…”

Section: Introductionmentioning

confidence: 99%

Artificial Neural Network Prediction of Retention of Amino Acids in Reversed-Phase HPLC under Application of Linear Organic Modifier Gradients and/or pH Gradients

D’Archivio

2019

Molecules

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A multi-layer artificial neural network (ANN) was used to model the retention behavior of 16 o-phthalaldehyde derivatives of amino acids in reversed-phase liquid chromatography under application of various gradient elution modes. The retention data, taken from literature, were collected in acetonitrile–water eluents under application of linear organic modifier gradients (φ gradients), pH gradients, or double pH/φ gradients. At first, retention data collected in φ gradients and pH gradients were modeled separately, while these were successively combined in one dataset and fitted simultaneously. Specific ANN-based models were generated by combining the descriptors of the gradient profiles with 16 inputs representing the amino acids and providing the retention time of these solutes as the response. Categorical “bit-string” descriptors were adopted to identify the solutes, which allowed simultaneously modeling the retention times of all 16 target amino acids. The ANN-based models tested on external gradients provided mean errors for the predicted retention times of 1.1% (φ gradients), 1.4% (pH gradients), 2.5% (combined φ and pH gradients), and 2.5% (double pH/φ gradients). The accuracy of ANN prediction was better than that previously obtained by fitting of the same data with retention models based on the solution of the fundamental equation of gradient elution.

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HPLC with diode‐array detection for the simultaneous determination of di(2‐ethylhexyl)phthalate and mono(2‐ethylhexyl)phthalate in seminal plasma

Cited by 20 publications

References 27 publications

Di-(2-Ethylhexyl) Phthalate and Autism Spectrum Disorders

Di-(2-Ethylhexyl) Phthalate and Autism Spectrum Disorders

Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid–liquid microextraction combined with high performance liquid chromatography

Artificial Neural Network Prediction of Retention of Amino Acids in Reversed-Phase HPLC under Application of Linear Organic Modifier Gradients and/or pH Gradients

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