HPLC-UV vs. UPLC-DAD for Estimation of Tinidazole, Benzyl Alcohol and Hydrocortisone Acetate Simultaneously with Tioconazole and Its Related Impurities in Bulk and Pharmaceutical Formulations

Elkady, Ehab F.; Tammam, Marwa Hosny; Elmaaty, Ayman Abo

doi:10.1080/22297928.2017.1308269

Cited by 5 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The here-obtained LOD was similar to [ 22 – 24 ] or even 10-times lower than that obtained by other authors using HPLC–DAD [ 25 ] or CE-UV [ 26 ]. Although, the LOD obtained using the proposed sensor was higher than those obtained in other HPLC methods [ 18 , 27 ] it should be noted that electrochemical methods are simpler, faster and cheaper than those based on chromatography, and reagent consumption, specially of organic solvents, is much lower. The GC–MS methods present very low LOD values, as can be expected considering the characteristics of MS detector.…”

Section: Resultsmentioning

confidence: 75%

Electrochemical sensing platform with gold nanoparticles capped by PDDA for benzyl alcohol determination

et al. 2023

View full text Add to dashboard Cite

An electrochemical sensor has been developed, by modifying screen-printed carbon devices (SPCE) with photochemically synthesized gold nanoparticles (AuNP), to determine benzyl alcohol, a preservative widely used in the cosmetic industry. To obtain the AuNP with the best properties for electrochemical sensing applications, the photochemical synthesis was optimized using chemometric tools. A response surface methodology based on central composite design was used to optimize the synthesis conditions, as irradiation time, and the concentrations of metal precursor and the capping/reducing agent (poly(diallyldimethylammonium) chloride, PDDA). The anodic current of benzyl alcohol on SPCE modified with the AuNP was used as response of the system. The best electrochemical responses were obtained using the AuNP generated by irradiating for 18 min a 7.20 $$\times$$ × 10−4 mol L−1 AuCl4−-1.7% PDDA solution. The AuNP were characterized by transmission electron microscopy, cyclic voltammetry and dynamic light scattering. The nanocomposite-based sensor formed by the optimal AuNP (AuNP@PDDA/SPCE) was used to determine benzyl alcohol by linear sweep voltammetry in 0.10 mol L−1 KOH. The anodic current at + 0.017 ± 0.003 V (vs. AgCl) was used as analytical signal. Detection limit obtained under these conditions was 2.8 µg mL−1. The AuNP@PDDA/SPCE was applied to determine benzyl alcohol in cosmetic samples. Graphical Abstract

show abstract

Section: Resultsmentioning

confidence: 75%

Electrochemical sensing platform with gold nanoparticles capped by PDDA for benzyl alcohol determination

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The eluent gradient program was as follows: 0/50, 2/70, 5.6/70, 5.7/50, 7/50 (time [min]/%B). The established conditions and spectrophotometric detection in UV at a wavelength of 220 nm also allowed for the analysis of impurities present in the tested material [106]. Clotrimazole in substance and human plasma was analyzed on an Acquity BEH C-18 (50 × 2.1 mm, 1.7 µm) column and a mixture of water with 0.2% ammonium acetate and 0.1% formic acid (A) and methanol (B) in a volume ratio 18:82 (A:B).…”

Section: Antifungal and Anthelmintic Drugsmentioning

confidence: 99%

UPLC Technique in Pharmacy—An Important Tool of the Modern Analyst

et al. 2022

View full text Add to dashboard Cite

In recent years, ultra-efficient liquid chromatography (UPLC) has gained particular popularity due to the possibility of faster separation of small molecules. This technique, used to separate the ingredients present in multi-component mixtures, has found application in many fields, such as chemistry, pharmacy, food, and biochemistry. It is an important tool in both research and production. UPLC created new possibilities for analytical separation without reducing the quality of the obtained results. This technique is therefore a milestone in liquid chromatography. Thanks to the increased resolution, new analytical procedures, in many cases, based on existing methods, are being developed, eliminating the need for re-analysis. Researchers are trying to modify and transfer the analytical conditions from the commonly used HPLC method to UPLC. This topic may be of strategic importance in the analysis of medicinal substances. The information contained in this manuscript indicates the importance of the UPLC technique in drug analysis. The information gathered highlights the importance of selecting the appropriate drug control tools. We focused on drugs commonly used in medicine that belong to various pharmacological groups. Rational prescribing based on clinical pharmacology is essential if the right drug is to be administered to the right patient at the right time. The presented data is to assist the analyst in the field of broadly understood quality control, which is very important, especially for human health and treatment. This manuscript shows that the UPLC technique is now an increasingly used tool for assessing the quality of drugs and determining the identity and content of active substances. It also allows the monitoring of active substances and finished products during their processing and storage.

show abstract

“…To separate and then determine the components of combination drugs, high- and ultrahigh-performance liquid chromatography (LC) [ 4 , 5 , 6 , 7 , 8 ], microemulsion LC [ 9 ], hydrophilic interaction LC [ 10 ], and capillary electrophoresis [ 11 , 12 ] are used. The preliminary separation of active components makes the analysis difficult, time-consuming, expensive, and often polluting.…”

Section: Introductionmentioning

confidence: 99%

Perfluorosulfonic Acid Membranes Modified with Polyaniline and Hydrothermally Treated for Potentiometric Sensor Arrays for the Analysis of Combination Drugs

et al. 2023

View full text Add to dashboard Cite

A novel potentiometric multisensory system for the analysis of sulfamethoxazole and trimethoprim combination drugs was developed. The potentiometric sensors (Donnan potential (DP) was used as an analytical signal) with an inner reference solution were based on perfluorosulfonic acid (PFSA) membranes modified with polyaniline (PANI) by in situ oxidative polymerization. The order of the membrane treatment with precursor solutions and their concentrations was varied. Additionally, the PFSA/PANI composite membranes were hydrothermally treated at 120 °C. The influence of the preparation conditions and the composition of membranes on their sorption and transport properties was studied. We estimated the factors affecting the sensitivity of DP-sensors based on the PFSA/PANI composite membranes to ions of sulfamethoxazole and trimethoprim simultaneously presented in solutions. A developed multisensory system provided a simultaneous determination of two analytes in aqueous solutions without preliminary separation, derivatization, or probe treatment. The re-estimation of the calibration characteristics of the multisensory system did not show a statistically significant difference after a year of its use. The limits of detection of sulfamethoxazole and trimethoprim were 1.4 × 10−6 and 8.5 × 10−8 M, while the relative errors of their determination in the combination drug were 4 and 5% (at 5 and 6% relative standard deviation), respectively.

show abstract

HPLC-UV vs. UPLC-DAD for Estimation of Tinidazole, Benzyl Alcohol and Hydrocortisone Acetate Simultaneously with Tioconazole and Its Related Impurities in Bulk and Pharmaceutical Formulations

Cited by 5 publications

References 20 publications

Electrochemical sensing platform with gold nanoparticles capped by PDDA for benzyl alcohol determination

Electrochemical sensing platform with gold nanoparticles capped by PDDA for benzyl alcohol determination

UPLC Technique in Pharmacy—An Important Tool of the Modern Analyst

Perfluorosulfonic Acid Membranes Modified with Polyaniline and Hydrothermally Treated for Potentiometric Sensor Arrays for the Analysis of Combination Drugs

Contact Info

Product

Resources

About