HPLC-UV Method Development and Validation for the Determination of Low Level Formaldehyde in a Drug Substance

Soman, Ashish; Qiu, Yu-xin; Li, Q. Chan

doi:10.1093/chromsci/46.6.461

Cited by 86 publications

(56 citation statements)

References 7 publications

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“…Formalin and H 2 O 2 were purchased from Sigma (St. Louis, MO, USA), and actual concentrations were assessed before use by HPLC-UV (Soman et al 2008) and peroxidase-H 2 O 2 analysis kit (Cell Biolabs, San Diego, CA), respectively. All other reagents were purchased from Sigma if not specified otherwise.…”

Section: Chemicalsmentioning

confidence: 99%

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Biochemical changes and drug residues in ascidian Halocynthia roretzi after formalin–hydrogen peroxide treatment regimen designed against soft tunic syndrome

et al. 2017

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Soft tunic syndrome (STS) is a protozoal disease caused by Azumiobodo hoyamushi in the edible ascidian Halocynthia roretzi. Previous studies have proven that combined formalin-hydrogen peroxide (H 2 O 2 ) bath is effective in reducing STS progress and mortality. To secure target animal safety for field applications, toxicity of the treatment needs to be evaluated. Healthy ascidians were bathed for 1 week, 1 h a day at various bathing concentrations. Bathing with 5-and 10-fold optimum concentration caused 100% mortality of ascidians, whereas mortality by 0.5-to 2.0-fold solutions was not different from that of control. Of the oxidative damage parameters, MDA levels did not change after 0.5-and 1.0-fold bathing. However, free radical scavenging ability and reducing power were significantly decreased even with the lower-than-optimal 0.5-fold concentration. Glycogen content tended to increase with 1-fold bathing without statistical significance. All changes induced by the 2-fold bathing were completely or partially restored to control levels 48 h post-bathing. Free amino acid analysis revealed a concentration-dependent decline in aspartic acid and cysteine levels. In contrast, alanine and valine levels increased after the 2-fold bath treatment. These data indicate that the currently established effective disinfectant regimen against the parasitic pathogen is generally safe, and the biochemical changes observed are transient, lasting approximately 48 h at most. Low levels of formalin and H 2 O 2 were detectable 1 h post-bathing; however, the compounds were completely undetectable after 48 h of bathing. Formalin-H 2 O 2 bathing is effective against STS; however, reasonable care is required in the treatment to avoid unwanted toxicity. Drug residues do not present a concern for consumer safety.

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Section: Chemicalsmentioning

confidence: 99%

“…The ascidian tissues were homogenized in two volumes of distilled water and centrifuged to obtain supernatants. Formalin content was analyzed by HPLC-UV following complex formation with 2,4-dinitrophenylhydrazine (Soman et al 2008). The limit of detection sensitivity was approximately 500 nM.…”

Section: Analyses Of Formalin and H 2 Omentioning

confidence: 99%

Biochemical changes and drug residues in ascidian Halocynthia roretzi after formalin–hydrogen peroxide treatment regimen designed against soft tunic syndrome

et al. 2017

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“…Most commonly used method for the collection of airborne CC is to use an adsorbing material with a derivatization agent to capture CC, followed by solvent (Soman et al, 2008) or thermal desorption (Ho, et al, 2004) and analysis using a chromatographic or spectroscopic methods (Szulejko, et al, 2015). Various derivatization materials are reported by many research groups (Szulejko et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of a paper-based approach for the detection of carbonyl compounds in indoor air samples

Madavie

Kaumal

2017

Sri Lankan J. Biol.

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This study contributes to the development of a method to measure the level of airborne carbonyl compounds. Carbonyl compounds (CC) are very common indoor air pollutants which are widely used in building and home furnishing industries. Most CC off-gas or vaporize even at normal room temperatures, which results in an increase in the indoor volatile organic carbon levels. Acute and chronic exposure to formaldehyde can result in respiratory symptoms and eye, nose, and throat irritation in humans. Because of these adverse health effects and the increased levels of CC in indoor air and residential areas, there has been a considerable attention toward the development of sensitive methods to monitor indoor CC levels. Most methods available for monitoring indoor CC levels are indirect and time consuming. This paper describes a novel, portable, on site, low-cost and green method for regular monitoring of CC in air. In this method, the derivatization reagent, 2,4-dinitrophenylhydrazine (2,4-DNPH) was used to capture the airborne Cc. Formaldehyde was used as the CC for this study. A novel paper-based platform soaked with 2,4 DNPH was used for the quantification of air born cc. Derivatives of CC were formed on a paper-based platform which were then directly analyzed using FTIR or an image scanner. The FTIR response for the carbonyl derivatives, which correlates with the levels of airborne CC, was used to quantify the levels of airborne cc. The same was scanned using a high resolution image scanner to obtain the RGB values and the yellow color intensity was calculated. Based on the RGB values, the produced yellow color intensity and the levels of CC produced a linear relationship. The use of an image scanner to quantify the levels of CC makes this method a portable, sensitive and on-site CC detection method. This newly developed paperbased FTIR method is agreeable with the stranded HPLC method at 95% confidence limit.

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“…However, very few HPLC methods are reported for the analysis of aldehyde using a derivatization reaction with DNPH in drug substances [13][14][15] and blood plasma [16] . To our knowledge no HPLC method is reported for quantitative determination of ACD in CC.…”

mentioning

confidence: 99%

Determination of Acetaldehyde Content in Candesartan Cilexetil by HPLC

Kumar¹,

Srivastava²

2018

pharmaceutical-sciences

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HPLC-UV Method Development and Validation for the Determination of Low Level Formaldehyde in a Drug Substance

Cited by 86 publications

References 7 publications

Biochemical changes and drug residues in ascidian Halocynthia roretzi after formalin–hydrogen peroxide treatment regimen designed against soft tunic syndrome

Biochemical changes and drug residues in ascidian Halocynthia roretzi after formalin–hydrogen peroxide treatment regimen designed against soft tunic syndrome

Development of a paper-based approach for the detection of carbonyl compounds in indoor air samples

Determination of Acetaldehyde Content in Candesartan Cilexetil by HPLC

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