HPLC-UV Method Development and Validation of Potato Sprout Inhibitor 1,4-Dimethylnaphthalene Using Different Systems

Abstract

1,4-Dimethylnaphthalene (1,4-DMN) is effective sprout suppressant used in potato stores in many countries in the world. High residue levels of this compound on the potatoes and in other environmental samples are considered for human health and environmental risks. Determination of the residue requires specific analytical methods to be developed and validated. In this study, HPLC-UV was selected for validating a separation method based on reversed phase for the analysis of 1,4-DMN using 2-methylnaphthalene (2-MeN) as internal standard testing three HPLC systems. Under the same chromatographic conditions, all three systems achieved good separation on a Jones column (Hypersil ODS 5 μm, 250 mm × 4.6 mm) at ambient temperature isocratically using 70% acetonitrile as mobile phase at a flow rate of 1.5 mL min−1, 20 μL injection volume, a run time of 10 min, and a detection wavelength of 228 nm. All three systems showed high precision, good linearity, and low limit of detection (LOD) and quantification (LOQ); particularly, the SpectraSERIES UV100-autosampler system offered lower values of LOD (0.001–0.004 μg mL−1) and LOQ (0.002–0.013 μg mL−1) for both compounds. This system can be used for the quantitative determination of 1,4-DMN residue in potato and environmental samples.

Maleic and l-tartaric acids as new anti-sprouting agents for potatoes during storage in comparison to other efficient sprout suppressants

“…So, it is an important concern for storing harvested potatoes timely with proper sprout suppressants. The widely used CIPC has been reported to have some toxic effects on health as well as on environment due to their degraded by-products, uptake and residual effect on the tubers, low volatilization and less water solubility 15 , 16 , 26 , 33 . Residual effects of CIPC is noticed not only in the stored potatoes but also in the processed potato products 34 , 35 .…”

“…It was reported that up to 45% of applied CIPC is persistent in the soil besides adhering to tubers 14 . It was observed that the CIPC residue in peel samples were fairly high about 15–85 mg/kg 15 , 16 . CIPC renders sprout growth by blocking the spindle formation during cell division 17 , 18 .…”

Development and Validation of an HPLC Method for the Analysis of Chlorpropham and 3-Chloroaniline in Potato Extracts

“…Five replicate injections of 1 g/mL of a mixture of CIPC, IPC, and 3-CA were injected to measure the precision of the standard solution by calculating the relative standard deviation (RSD%) as described in recent study [19].…”

“…were estimated for three compounds as mentioned in recent study [19], applying two approaches including statistical regression of the low concentration range of 0.02-0.1 g/mL and ten replicate injections of mixed solution at the low concentration of 0.05 g/mL of CIPC, IPC, and 3-CA.…”