HPLC Separation of Triacylglycerol Positional Isomers on a Polymeric ODS Column

Homochiral Asymmetric Triacylglycerol Isomers in Egg Yolk

“…Several analytical methods for TAG isomers have been developed so far. Kuroda et al found that TAG-PIs binding at least one saturated FA can be separated on a polymeric ODS column 14 . We reported that TAG-PIs binding two saturated FAs can be separated within a short period using a C28 column 15 .…”

Rapid Separation of Triacylglycerol Positional Isomers Binding Two Saturated Fatty Acids Using Octacocyl Silylation Column

“…Because the silver-ion column discriminates between TAG-PIs having different number and positions of double bonds, it can separate the β-OPO/β-OOP pair. In the case of the polymeric ODS stationary phase, the slot model explains the separation mechanism of TAG-PIs 8,17 . The difference between C28 and ODS columns is the length of alkyl groups.…”

“…The characteristics of the elution order of silver-ion HPLC differ from those of reversed-phase HPLC, in which the elution order of TAG molecular species depends on the acyl-chain length and the number of double bonds 15 . We have previously reported that β-OPO/β-OOP and β-POP/β-PPO can be separated using a recycle HPLC system equipped with a non-endcapped polymeric octadecylsilane ODS column 8 .…”

Characterization of Non-endcapped Polymeric ODS Column for the Separation of Triacylglycerol Positional Isomers

“…Thus, it is likely that the TAG-PI pairs described above are essentially unresolved on the non-endcapped polymeric ODS stationary phase. Kuroda et al discussed 18) the resolution mechanism of TAG-PI on a polymeric ODS stationary phase using the "slot model" advocated by Wise and Sander 21) . That is, the ODS groups comprising the polymeric stationary phase must be arranged more closely to each other than those of other kinds of ODS stationary phases, and the space between the ODS group in polymeric ODS phase should be narrower than that in monomeric ODS phase.…”

“…They reported good resolution for six different TAG-PI pairs comprising two palmitic acids and one unsaturated fatty acid. Kuroda et al also demonstrated that a non-endcapped polymeric ODS column can separate TAG-PI pairs consisting of two palmitic acids and one oleic acid or one palmitic acid and two oleic acids 18) . These methods are very simple and reproducible and may be useful for the separation of TAG-PI.…”