HPLC Separation of Acetaminophen and its Impurities Using A Mixed-mode Reversed-Phase/Cation Exchange Stationary Phase

Călinescu, Octavian; Badea, Irinel Adriana; Vladescu, Luminita; Meltzer, Viorica; Pincu, Elena

doi:10.1093/chromsci/bmr043

Cited by 47 publications

(29 citation statements)

References 28 publications

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“…The elution time for acetaminophen detected from all the samples by UV-Vis at the wavelength of 230 nm was 2.30 min. The process-related organic impurities of acetaminophen have been studied and identified by using an HPLC method; they can result from the poor quality of starting materials, reagents and solvents, the reaction conditions and the design of the process equipment (Kamberi et al, 2004;Calinescu et al, 2012).…”

Section: Stability After Ald Coatingmentioning

confidence: 99%

Surface modification of acetaminophen particles by atomic layer deposition

Kääriäinen

Kemell

Vehkamäki

et al. 2017

International Journal of Pharmaceutics

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Active pharmaceutical ingredients (APIs) are predominantly organic solid powders. Due to their bulk properties many APIs require processing to improve pharmaceutical formulation and manufacturing in the preparation for various drug dosage forms. Improved powder flow and protection of the APIs are often anticipated characteristics in pharmaceutical manufacturing. In this work, we have modified acetaminophen particles with atomic layer deposition (ALD) by conformal nanometer scale coatings in a one-step coating process. According to the results, ALD, utilizing common chemistries for Al2O3, TiO2 and ZnO, is shown to be a promising coating method for solid pharmaceutical powders. Acetaminophen does not undergo degradation during the ALD coating process and maintains its stable polymorphic structure. Acetaminophen with nanometer scale ALD coatings shows slowed drug release. ALD TiO2 coated acetaminophen particles show cytocompatibility whereas those coated with thicker ZnO coatings exhibit the most cytotoxicity among the ALD materials under study when assessed in vitro by their effect on intestinal Caco-2 cells.

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Section: Stability After Ald Coatingmentioning

confidence: 99%

Surface modification of acetaminophen particles by atomic layer deposition

Kääriäinen

Kemell

Vehkamäki

et al. 2017

International Journal of Pharmaceutics

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“…Various methods including titrimetry , spectrophotometry capillary electrophoresis liquid chromatography , and electrochemical techniques have been used for determination of paracetamol. Among mentioned methods, titrimetric and spectrophotometric methods require early laborious extraction process, whereas liquid chromatography and capillary electrophoresis are time‐consuming and expensive.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Hexacyanoferrate Intercalated Ni/Al Layered Double Hydroxide Modified Electrode as a Sensitive Electrochemical Sensor for Paracetamol Determination

Asadpour‐Zeynali

Amini

2016

Electroanalysis

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An electrochemical sensor for paracetamol (PC) based on the hexacyanoferate(III) intercalated Ni−Al layered double hydroxide (Ni−Al−HCF) was presented. The as‐prepared LDH structurally and morphologically was characterized by scanning electron microscopy, X‐ray diffraction, and Fourier transform IR. Electrochemical studies revealed that Ni−Al−HCF film modified glassy carbon (GC) electrode exhibited remarkable electrocatalytic activity toward the oxidation of paracetamol. The electrochemical behavior of PC on the Ni−Al−HCF film was investigated in detail. Under optimum experimental conditions, the electrocatalytic response of the modified GC electrode was linear in the PC concentration range 3×10−6−–1.5×10−3 mol L−1, with a detection limit of 8×10−7 mol L−1 (S/N=3), using hydrodynamic amperometry. In addition, the modified electrode exhibited good reproducibility, long‐term stability and anti‐interference property. The fabricated sensor was successfully applied to determination of PC in various pharmaceutical preparations such as tablets, oral solution, and oral drops. Finally, the method was validated by the analysis of paracetamol spiked human serum samples, and good recoveries were obtained in the range of 99.2–103 %.

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“…[1,46,47] The PK of ACP is well studied in different biological matrices; [46] however, only a few limited papers report the application of microsampling techniques for ACP PK studies. The most applied analytical technique for the quantitative measurement of ACP concentration levels in biological fluids is liquid chromatography (LC) in combination with different detectors such as ultraviolet (UV), [48,49] UV in combination with electrochemical detection (ED), [50] fluorescence detection (FD), [51] and MS detection, [32,52] although spectrophotometry can also be applied. [53] In the last 20 years, LC-MS assays have outgrown the mainly applied LC-UV assays in drug analysis and drug development.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of peripheral blood microsampling techniques in combination with liquid chromatography‐high resolution mass spectrometry for the determination of drug pharmacokinetics in clinical studies

Rincón

Meesters

2013

Drug Testing and Analysis

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New bioanalytical assays were developed, validated, and applied in a clinical study for quantitative measurement of acetaminophen concentrations in blood and plasma samples. Furthermore, after validation, the bioanalytical assays were used for determination of pharmacokinetics within a group of six healthy male human volunteers after admission of a single oral dose of 500 mg of acetaminophen. Quantitative analyses were done by means of liquid chromatography-high resolution mass spectrometry and blood samples were collected at various sampling time points using different peripheral blood microsampling techniques. Post-dose peripheral collected blood samples were applied for the preparation of dry blood spots, dried matrix on paper discs, and peripheral plasma. Pharmacokinetic parameters determined were clearance (Cl), area under the curve (AUC), volume of distribution (Vd ), peak concentration (Cmax ), time of occurrence of peak concentration (Tmax ) and half-life time (T½ ). Observed pharmacokinetic values were not statistically (ANOVA) different compared to in literature reported values based on venous blood collection. The present pilot study demonstrated the feasibility of peripheral blood microsampling techniques in combination with quantitative liquid chromatography-high resolution mass spectrometry analysis for the determination of pharmacokinetics in clinical studies.

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HPLC Separation of Acetaminophen and its Impurities Using A Mixed-mode Reversed-Phase/Cation Exchange Stationary Phase

Cited by 47 publications

References 28 publications

Surface modification of acetaminophen particles by atomic layer deposition

Surface modification of acetaminophen particles by atomic layer deposition

Nanostructured Hexacyanoferrate Intercalated Ni/Al Layered Double Hydroxide Modified Electrode as a Sensitive Electrochemical Sensor for Paracetamol Determination

Evaluation of peripheral blood microsampling techniques in combination with liquid chromatography‐high resolution mass spectrometry for the determination of drug pharmacokinetics in clinical studies

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