HPLC Qualitative Amino Acid Analysis in the Clinical Laboratories

Rutledge, Joe C.; Rudy, J L

doi:10.1093/ajcp/87.5.614

Cited by 9 publications

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“…Over the past decade, a variety of high performance liquid chromatography (HPLC) methods have been developed for the determination of the amino acid composition of biological materials (Bildingmeyer et al, 1984;Vendrell and Aviles, 1986;Rutledge and Rudy, 1987). We have previously developed a HPLC method for hydroxyproline analysis, and have identified hydroxyproline in rat tail collagen and in conditioned medium (Ikeda et al, 1991;1993), urine (lkeda et al, 1995 and cultured rat hepatoma cells (M) .…”

Section: Introductionmentioning

confidence: 99%

The amino acid composition of mammalian and bacterial cells

et al. 1997

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Summary. High performance liquid chromatography was used to analyze the amino acid composition of cells. A total of 17 amino acids was analyzed. This method was used to compare the amino acid compositions of the following combinations: primary culture and established cells, normal and transformed cells, mammalian and bacterial cells, and Escherichia coli and Staphylococcus aureus. The amino acid compositions of mammalian cells were similar, but the amino acid compositions of Escherichia coli and Staphylococcus aureus differed not only from mammalian cells, but also from each other. It was concluded that amino acid composition is almost independent of cell establishment and cell transformation, and that the amino acid compositions of mammalian and bacterial cells differ. Thus, it is likely that changes in amino acid composition due to cell transformation or species differences between mammalian cells are negligible compared with the differences between mammalian and bacterial cells, which are more distantly related.

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Section: Introductionmentioning

confidence: 99%

The amino acid composition of mammalian and bacterial cells

et al. 1997

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“…The yield of Dns-amino acid under the classical conditions of the reaction (acetone/water 2:1, pH 9, sodium bicarbonate buffer) was found to be dependent on the relative amount of Dns-Cl for some amino acids [26], while according to the Tapuhi method [27] with substitution of acetonitrile for acetone as a solvent, such a dependence was not observed. In this work, acetonitrile was used as a solvent for dansylation.…”

Section: Optimization Of the Procedures For Dansyl Derivatizationmentioning

confidence: 92%

Simultaneous RP-HPLC-DAD determination of dansyl amino acids in chemically treated human hair

Sherovski

Стефова

Ristovska

2018

Maced. J. Chem. Chem. Eng.

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A simple, rapid and reliable RP-HPLC-DAD method for the analysis of 19 amino acids in the protein hydrolysate of chemically treated hair is developed. The pre-column dansylation and RP-HPLC separation were optimized and peak resolution was improved as compared to previously published HPLC methods. The developed method showed excellent linearity for all tested amino acids in the concentration range 0.05-0.5 mmol/l. The obtained results show that the concentration of all amino acids detected in bleached hair decreases with increasing the concentration of hydrogen peroxide, this is most significant for cystine, compared to virgin hair. Higher amounts of the typical amino acids for keratin have been measured after exposure of hair to protein treatments. The method has been shown as very useful for amino acids analysis in a protein hydrolisate and it can be also used for establishing the amino acid profiles of other protein samples using this optimized procedure.

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Amino acid measurement by high-performance liquid chromatography using electrochemical detection

Sherwood¹

1990

Journal of Neuroscience Methods

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HPLC Qualitative Amino Acid Analysis in the Clinical Laboratories

Cited by 9 publications

References 0 publications

The amino acid composition of mammalian and bacterial cells

The amino acid composition of mammalian and bacterial cells

Simultaneous RP-HPLC-DAD determination of dansyl amino acids in chemically treated human hair

Amino acid measurement by high-performance liquid chromatography using electrochemical detection

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