HPLC profile and simultaneous quantitative analysis of tingenone and pristimerin in four Celastraceae species using HPLC-UV-DAD-MS

Araujo-León, Jesús Alfredo; Cantillo-Ciau, Zulema; Ruiz-Ciau, Durcy; Coral-Martínez, Tania Isolina

doi:10.1016/j.bjp.2018.12.009

Cited by 12 publications

(5 citation statements)

References 30 publications

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“…A 10 μL aliquot of the mixed standard solution was precisely prepared for HPLC, as described in the ‘Preparation of test and standard solutions’ section, and was injected six times continuously within 1 day. The peak area of each component was recorded ( Alfredo et al, 2019 ). The results showed that the relative standard deviation of each standard substance was <3%, indicating good intraday precision of the instrument, as shown in Supplementary Table S1 .…”

Section: Resultsmentioning

confidence: 99%

Qualitative and quantitative analyses of chemical constituents in vitro and in vivo and systematic evaluation of the pharmacological effects of Tibetan medicine Zhixue Zhentong capsules

et al. 2023

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Introduction: Zhixue Zhentong capsules (ZXZTCs) are a Tibetan medicine preparation solely composed of Lamiophlomis rotata (Benth.) Kudo. L. rotata is the only species of the genus Laniophlomis (family Lamiaceae) that has medicinal constituents derived from the grass or root and rhizome. L. rotata is one of the most extensively used folk medicines by Tibetan, Mongolian, Naxi, and other ethnic groups in China and has been listed as a first-class endangered Tibetan medicine. The biological effects of the plant include hemostasis, analgesia, and the removal of blood stasis and swelling.Purpose: This study aimed to profile the overall metabolites of ZXZTCs and those entering the blood. Moreover, the contents of six metabolites were measured and the hemostatic, analgesic, and anti-inflammatory effects of ZXZTCs were explored.Methods: Ultra-performance liquid chromatography–tandem quadrupole time-of-flight high-resolution mass spectrometry (UPLC-Q-TOF-MS) was employed for qualitative analysis of the metabolites of ZXZTCs and those entering the blood. Six metabolites of ZXZTCs were quantitatively determined via high-performance liquid chromatography The hemostatic, analgesic, and anti-inflammatory effects of ZXZTCs were evaluated in various animal models.Results: A total of 36 metabolites of ZXZTCs were identified, including 13 iridoid glycosides, 9 flavonoids, 9 phenylethanol glycosides, 4 phenylpropanoids, and 1 other metabolite. Overall, 11 metabolites of ZXZTCs entered the blood of normal rats. Quantitative analysis of the six main metabolites, shanzhiside methyl ester, chlorogenic acid, 8-O-acetyl shanzhiside methyl ester, forsythin B, luteoloside, and verbascoside, was extensively performed. ZXZTCs exerted hemostatic effects by reducing platelet aggregation and thrombosis and shortening bleeding time. Additionally, ZXZTCs clearly had an analgesic effect, as observed through the prolongation of the latency of writhing, reduction in writhing, and increase in the pain threshold of experimental rats. Furthermore, significant anti-inflammatory effects of ZXZTCs were observed, including a reduction in capillary permeability, the inhibition of foot swelling, and a reduction in the proliferation of granulation tissue.Conclusion: Speculative identification of the overall metabolites of ZXZTCs and those entering the blood can provide a foundation for determining its biologically active constituents. The established method is simple and reproducible and can help improve the quality control level of ZXZTCs as a medicinal product. Evaluating the hemostatic, analgesic, and anti-inflammatory activities of ZXZTCs can help reveal its mechanism.

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Section: Resultsmentioning

confidence: 99%

Qualitative and quantitative analyses of chemical constituents in vitro and in vivo and systematic evaluation of the pharmacological effects of Tibetan medicine Zhixue Zhentong capsules

et al. 2023

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“…Conventionally, in triterpene extractions from plants, the use of hazard/toxic solvents such as hexane, chloroform, and petroleum ether has been preferred. , Nonetheless, their extraction with environmentally friendly solvents such as ethanol has been recently encouraged. , On its part, ethanol is recognized as a green solvent, and it has been ranked as “recommended” for the unified version of general solvent selection guides for medicinal chemists and Sanofi’s solvent selection guide; this because of its advantageous properties among which are its low volatility, moderate solubility, low toxicity, and low cost. − …”

Section: Resultsmentioning

confidence: 99%

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

González-Félix

Mejía‐Manzano

Barba-Dávila

et al. 2021

Ind. Eng. Chem. Res.

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The natural triterpene pristimerin possesses interesting potential against several types of cancer. However, its production at the industrial level requires novel environmentally friendly and efficient extraction procedures. Here, we analyze an ethanol–phosphate aqueous two-phase system and the effect of its design parameters such as the tie-line length (TLL) and volume ratio (V R), on the direct extraction of pristimerin. The results showed that a high TLL value of 70% (w/w) and a low V R value of 0.33 allow the obtention of optimal yield and purity values. Partition experiments provided a compound K P value of 3.12 ± 1.21, reaffirming its preference for the ethanol-rich phase. Besides, green assessment positioned the extraction in classification A with 90.93 of 100 points. Furthermore, analysis at different laboratory scales (1×, 10×, and 100×) demonstrated scalability and robustness. Finally, a complete bioprocess based on the obtained results is suggested as a tool to address the pharmaceutical potential of this molecule sustainably and efficiently.

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“…Spectral data were collected at a detection wavelength of 220 nm for analysis. 16 Enzyme inhibitory studies a-Amylase inhibition. The inhibitory effect of the avonoidrich extract of B. vulgaris roots on a-amylase was assessed using the methodology described elsewhere.…”

Section: High-performance Liquid Chromatography (Hplc-dad) Analysismentioning

confidence: 99%

Exploring beetroot (Beta vulgaris L.) for diabetes mellitus and Alzheimer's disease dual therapy: in vitro and computational studies

Ojo,

Gyebi,

Ezenabor

et al. 2024

RSC Adv.

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HPLC profile and simultaneous quantitative analysis of tingenone and pristimerin in four Celastraceae species using HPLC-UV-DAD-MS

Cited by 12 publications

References 30 publications

Qualitative and quantitative analyses of chemical constituents in vitro and in vivo and systematic evaluation of the pharmacological effects of Tibetan medicine Zhixue Zhentong capsules

Qualitative and quantitative analyses of chemical constituents in vitro and in vivo and systematic evaluation of the pharmacological effects of Tibetan medicine Zhixue Zhentong capsules

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

Exploring beetroot (Beta vulgaris L.) for diabetes mellitus and Alzheimer's disease dual therapy: in vitro and computational studies

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