HPLC profile and antibiotic-modifying activity of Azadirachta indica A. Juss, (Meliaceae)

Cristo, Jannyketchuly S.; Matias, Edinardo Fágner Ferreira; Figueredo, Fernando Gomes; Santos, Joycy Francely Sampaio dos; Pereira, Nara Luana Ferreira; G.A.S., José; Aquino, Pedro Éverson Alexandre de; Nogueira, Maria N.F.; Ribeiro-Filho, Jaime; Cunha, Francisco Assis Bezerra da; Costa, Maria do Socorro; Campina, Fábia F.; Tintino, Saulo Relison; Salgueiro, Cristiane Clemente de Mello; Coutinho, Henrique Douglas Melo

doi:10.1016/j.indcrop.2016.10.001

Cited by 19 publications

(8 citation statements)

References 16 publications

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“…The outcome of combining two drugs can be synergistic, additive, or antagonistic depending on the interaction between drugs. Various in vitro experiments have established the fact that a combination of plant extracts and antibiotics possess a synergistic effect, which results in a significant decrease in levels of MIC for the antibiotics [48,49]. One of the main objectives of the current study was to estimate and establish the combined antibacterial activity of plant extracts with antibiotics against clinical strains of periodontal disease causing bacteria.…”

Section: Discussionmentioning

confidence: 99%

Evaluation and Comparison of Antibacterial Efficacy of Herbal Extracts in Combination with Antibiotics on Periodontal pathobionts: An in vitro Microbiological Study

et al. 2019

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Background: In the past few decades focus of research has been toward herbal medicines because of growing bacterial resistance and side effects of antimicrobial agents. The extract derived from the plants may increase the efficacy of antibiotics when used in combination against pathogenic bacteria. In the current study, the synergistic antibacterial efficacy of plant extracts in combination with antibiotics has been assessed on selected periodontal pathogens. Methods: Ethanolic extracts were prepared from Salvadora persica (Miswak) and Cinnamomum zeylanicum (Ceylon cinnamon), by the soxhalate method. Plaque samples were collected from clinical periodontitis patients to isolate and grow the periodontal pathobionts under favorable conditions. Susceptibility of bacteria to the extracts was assessed by gauging the diameter of the inhibition zones. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts were determined against each bacterium. Synergistic activity of plants extract in combination with antibiotics against the bacteria was also assessed by measuring the diameter of the inhibition zones. Results: Ethanolic extract of both the plants showed an inhibitory effect on the proliferation and growth of all four strains of periodontal pathobionts. Maximum antibacterial activity was exhibited by C. zeylanicum against Tannerella forsythia (MIC = 1.56 ± 0.24 mg/mL, MBC = 6.25 ± 0.68 mg/mL), whereas among all the studied groups the minimum activity was reported by C. zeylanicum against Aggregatibacter actinomycetemcomitans the (MIC = 12.5 ± 3.25 mg/mL, MBC = 75 ± 8.23 mg/mL). Combination of herbal extracts with different antibiotics revealed a synergistic antibacterial effect. The best synergism was exhibited by S. persica with metronidazole against A. actinomycetemcomitans (27 ± 1.78). Conclusions: Current in vitro study showed variable antibacterial activity by experimented herbal extracts against periodontal pathobionts. The synergistic test showed significant antibacterial activity when plant extracts were combined with antibiotics.

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Section: Discussionmentioning

confidence: 99%

Evaluation and Comparison of Antibacterial Efficacy of Herbal Extracts in Combination with Antibiotics on Periodontal pathobionts: An in vitro Microbiological Study

et al. 2019

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“…Figura 2 -Histoquímica da lâmina foliar de Azadirachta indica A.Juss. CRISTO et al, 2016;WANG et al, 2016). Algumas destas substâncias estão relacionadas a atividades biológicas, como o nimbolídeo, que possui atividade anticâncer (WANG et al, 2016), e a azadiractina, que apresenta atividade inseticida (MATOS, 2007).…”

Section: Resultsunclassified

CARACTERIZAÇÃO ANATÔMICA E HISTOQUÍMICA DA LÂMINA FOLIAR DE Calotropis procera (Aiton) W.T.Aiton

Sá¹,

Silva²,

Santos³

et al. 2019

Biomedicina E Farmácia: Aproximações 2

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“…The resultant outcome of combining two drugs can be synergistic, additive or antagonistic. Various in vitro experiments have established the fact that a combination of plant extracts and antibiotics possess a synergistic effect, which results in a significant decrease in levels of MIC for the antibiotics [47,48]. One of the main objectives of the present study was to evaluate and establish the combined effects of antimicrobial agents with plant extracts against clinical strains of periodontopathic bacteria.…”

Section: Discussionmentioning

confidence: 96%

Synergistic antibacterial activity of herbal extracts with antibiotics on bacteria responsible for periodontitis

Saquib

AlQahtani

Ahmad

et al. 2021

J Infect Dev Ctries

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Introduction: Development of bacterial resistance and antimicrobial side-effect has shifted the focus of research toward Ethnopharmacology. A biologically active compound derived from the plants may increase the effectiveness of antibiotic when used in combination. The present study aims to determine the synergistic antibacterial effect of ethanolic extracts of Punica granatum (pericarp), Commiphora molmol, Azadirachta indica (bark) in combination with amoxicillin, metronidazole, tetracycline, and azithromycin on periodontopathic bacteria: Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans. Methodology: Periodontopathic bacterial strains were isolated from the plaque sample that was collected from periodontitis patients and grown under favorable conditions. Susceptibility of bacteria to the antibiotics and extracts was determined by disc diffusion method by measuring the diameter of the inhibition zones. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts were evaluated against each bacterium. Synergistic effect of plant extract in combination with antibiotics was tested against each bacterium by measuring the diameter of zone of inhibition (ZOI). Results: Findings revealed that all plant extracts exhibited an inhibitory effects on the proliferation and growth of periodontopathic bacteria. The maximum antibacterial effect was exhibited by C. molmol on P. gingivalis (ZOI = 20 ± 0.55 mm, MIC = 0.53 ± 0.24 mg/mL and MBC = 5.21 ± 1.81 mg/mL) (p < 0.05), meanwhile, no antibacterial activity was exhibited by P. granatum on T. forsythia. Synergistic antibacterial effect was recorded when plant extracts were used in combination with antibiotics. The best synergism was exhibited by P. granatum with amoxicillin against A. actinomycetemcomitans (24 ± 1.00 mm) (p < 0.05). Conclusions: The synergistic test showed significant antibacterial activity when plant extracts were combined with antibiotics against all the experimented bacteria.

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HPLC profile and antibiotic-modifying activity of Azadirachta indica A. Juss, (Meliaceae)

Cited by 19 publications

References 16 publications

Evaluation and Comparison of Antibacterial Efficacy of Herbal Extracts in Combination with Antibiotics on Periodontal pathobionts: An in vitro Microbiological Study

Evaluation and Comparison of Antibacterial Efficacy of Herbal Extracts in Combination with Antibiotics on Periodontal pathobionts: An in vitro Microbiological Study

CARACTERIZAÇÃO ANATÔMICA E HISTOQUÍMICA DA LÂMINA FOLIAR DE Calotropis procera (Aiton) W.T.Aiton

Synergistic antibacterial activity of herbal extracts with antibiotics on bacteria responsible for periodontitis

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