HPLC Profile Analysis of Hepatoprotective Oleanene-Glucuronides in Puerariae Flos.

Abstract

In order to confirm the constitution of hepatoprotective oleanene glucuronide (OG), HPLC profile analyses of the total OG fractions of both Puerariae Thomsonii Flos (the flowers of Pueraria thomsonii) and Puerariae Lobatae Flos (the flowers of P. lobata) were performed. No remarkable difference in the HPLC profiles with respect to OGs in the flowers was shown, in contrast to those of the roots. By repeated chromatography of the total OG fraction of Puerariae Thomsonii Flos, soyasaponin I (1), kaikasaponin III (2) and kakkasaponin I (3), which had been already isolated from Puerariae Lobatae Flos, were obtained. The hepatoprotective activity of 2 towards immunologically induced liver injury was significantly more effective than that of 1. This information supported previously obtained structure-hepatoprotective relationship data which was measured on another model. The structure-activity relationship information which suggested that the hydroxymethyl group of the galactosyl unit would enhance the hepatoprotective activity was also substantiated.

Microbial transformation and bioactivation of isoflavones from Pueraria flowers by human intestinal bacterial strains

“…Although the HPLC profiles for the total saponin fraction obtained from both types of Puerariae Flos had no remarkable differences between the two, the total isoflavone fraction of P. lobata was different from that of P. thomsonii [9]. The main isoflavones of P. thomsonii are tectorigenin-7-O-xylosylglucoside (1) and tectoridin (1a), whereas that of P. lobata is kakkalide (2) [10].…”

Simultaneous analysis of isoflavones and saponins in Pueraria flowers using HPLC coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside

“…Analysis was conducted according to the previously described protocol [8]. In a preliminary experiment, both types of constituents (saponin and isoflavone) were most efficiently extracted with 60% EtOH (EtOH/H 2 O = 6:4), when tested with various solvents (50-100% MeOH and 50-100% EtOH).…”

“…Some biological activities of Puerariae Flos and its constituents have already been elucidated, and include detoxification of alcohol [1][2][3][4][5][6], hepatoprotection [2,3,7,8], anticomplementary activity [9], and estrogenic activity [10]. Very recently, we reported microbial transformation of isoflavones and bioactivation by intestinal bacteria [11].…”

“…Although the HPLC profiles of the total saponin fraction obtained from both types of Puerariae Flos had no remarkable differences between the two [8], the total isoflavone fraction of P. lobata was different from that of P. thomsonii [12]. The isoflavones of P. thomsonii have an hydroxy group at the 4 0 -position, whereas those of P. lobata have a methoxy group at the same position [11].…”

“…It could be also used with short wavelengths, such as 205 nm, for analyzing the saponin fraction [8]. However, simultaneous analysis of the total saponin and isoflavone fraction has been difficult due to the difference in absorption coefficient between saponin and isoflavones.…”