HPLC of fatty acid esters of mono‐ and polyhydric alcohols. Part 1: Analytical separation

Henke, H.; Schubert, Jack

doi:10.1002/jhrc.1240030204

Cited by 13 publications

(3 citation statements)

References 24 publications

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“…Several methods for the separation of hydroxy fatty acids by high-performance liquid chromatography (HPLC) have been published (7)(8)(9)(10)(11)(12)(13)(14)(15). Unfortunately, the existing techniques for quantitative analysis of hydroxy fatty acids by HPLC are quite laborious and often not accurate.…”

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confidence: 99%

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Separation and quantitation of hydroxy and epoxy fatty acid by high‐performance liquid chromatography with an evaporative light‐scattering detector

Gérard

Moreau

Fett

et al. 1992

J Americ Oil Chem Soc

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A new high-performance liquid chromatography technique with an evaporative light-scattering detector (ELSD) has been developed for the separation and quantitative analysis of hydroxy and epoxy fatty acids. This method employs a gradual binary gradient (hexanefisopropanol) and ELSD detection. The minimum limit of detection is about 1 ~g and ratio of mass to signal is essentially linear in the range of 10 to 200 pg. This highperformance liquid chromatography (HPLC) technique is able to separate various positional isomers of monohydroxy and dihydroxy fatty acids and can also discriminate between monohydroxy, epoxy, epoxyhydroxy, dihydroxy and trihydroxy fatty acids.KEY WORDS: Dihydroxy fatty adds, epoxy fatty acids, evaporative llght-scattering detector, fatty acids, lipids, normal phase HPLC, ricinoleic acid, trihydroxy fatty acids.

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“…Refractive index detection (12,13) and UV detection at 210 nm (14) unpublished data) in a normal-phase HPLC system have been tested but they are not quantitative Radiolabelled molecules have been used in a reverse-phase HPLC system for the separation and detection of hydroxy fatty acids (15), but the individual peaks were not identified.…”

mentioning

confidence: 99%

Separation and quantitation of hydroxy and epoxy fatty acid by high‐performance liquid chromatography with an evaporative light‐scattering detector

Gérard

Moreau

Fett

et al. 1992

J Americ Oil Chem Soc

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“…The literature describes only one method of determination using the conventional gravimetric method in the United States Pharmacopeia (Tong Liu, 2018) and a few other techniques for individual determination of components of interest by LC/LC–MS (Katayama, Masuda, & Taniguchi, 1991; Czichocki, Much, & Vollhardt, 1983; Aserin, Frenkel, & Garti, 1984; Henke & Schubert, 1980; Terol et al, 2010; Guarrasi, Mangione, Sanfratello, Martorana, & Bulone, 2010; Webster et al, 2006; Jinmao et al, 1999; Zembrzuska, 2017) and GC (Feng, Wu, & Cao, 1999). These methods did not distinguish or determine the trace mixtures of residual fatty alcohols (C 10 –C 17 ).…”

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confidence: 99%

A novel RP‐HPLC refractive index detector method development and validation for determination of trace‐level alcohols (un‐sulfated) in sodium lauryl sulfate raw material

Katakam¹,

Dongala²

2020

Biomedical Chromatography

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The main aim of the study is to develop and validate a simple and rapid liquid chromatographic analytical method for simultaneous determination of trace level of un‐sulfated alcohol impurities in sodium lauryl sulfate using high‐performance liquid chromatography with a refractive index detector. The chromatographic separation was achieved using flow rate of 3.0 ml/min with a Waters Symmetry C18 (150 × 4.6 mm, 5 μm) column and 50°C as a column temperature. The mobile phase comprised milliQ water and acetonitrile in the ratio of 30:70 v/v respectively. The detection was performed using a refractive index detector at a sensitivity of 64. The resolutions among n‐decanol, n‐dodecanol, n‐tetradecanol, n‐hexadecanol, and n‐heptadecanol were found to be >4. Regression analysis showed a correlation coefficient for the stated compounds of >0.999. The validated HPLC method was utilized effectively for the estimation of un‐sulfated alcohols in quality control labs for commercial release of sodium lauryl sulfate.

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HPLC of fatty acid esters of mono‐ and polyhydric alcohols. Part 2: Preparative separation

Henke¹,

Dietrich²

1980

J. High Resol. Chromatogr.

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Liquid chromatography, HPLC Preparative separation of esters in RP methanoVwater systems Mono-, di-, tri-, tetraesters SummaryThis paper demonstrates how simply and rapidly fatty acid esters of mono-and polyhydric alcohols can be separated quantitatively in preparative quantities on Lobar@' RP-8 packed columns. After the separation of unknown mixtures, the isolated esters are identified from spectroscopic data (IR/NMR) and, after saponification of the ester components (fatty acids and alcohols), from the retention times of gas and high-pressure liquid chromatographic separations. Thus, in particular, sparingly volatile or nonvolatile partial esters of polyhydric alcohols, as well as the long-chained full esters, can be determined qualitatively and quantitatively.The following fatty acid esters of mono-and polyhydric alcohols have been separated: the i-propylesters of the laurinic and myristinic acids, the i-butyl-, i-octyl-and i-octadecyl-esters of the palmitinic and stearic acids, the mono-and di-fatty acid esters (of the 1,3-bis-(2-hydroxyethyl)-5,5-dimethylhydantoins, the mono-, di-and tri-esters of the trimethylalpropane and the full esters of the pentaerythrite.

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HPLC of fatty acid esters of mono‐ and polyhydric alcohols. Part 1: Analytical separation

Cited by 13 publications

References 24 publications

Separation and quantitation of hydroxy and epoxy fatty acid by high‐performance liquid chromatography with an evaporative light‐scattering detector

Separation and quantitation of hydroxy and epoxy fatty acid by high‐performance liquid chromatography with an evaporative light‐scattering detector

A novel RP‐HPLC refractive index detector method development and validation for determination of trace‐level alcohols (un‐sulfated) in sodium lauryl sulfate raw material

HPLC of fatty acid esters of mono‐ and polyhydric alcohols. Part 2: Preparative separation

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