HPLC, NMR and MALDI-TOF MS Analysis of Condensed Tannins from Lithocarpus glaber Leaves with Potent Free Radical Scavenging Activity

Zhang, Liang Liang; Lin, Yi Hui

doi:10.3390/molecules13122986

Cited by 59 publications

(45 citation statements)

References 32 publications

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“…In the analysis of both condensed and hydrolysable tannins, matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) was used very successfully to determine the chemical composition of complex plant extracts 10–15. The analysis of tannins using MALDI‐TOF is a relatively new technique and provides additional information on the structure and molar mass distributions in a single experiment.…”

Section: Introductionmentioning

confidence: 99%

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MALDI‐TOF‐CID for the microstructure elucidation of polymeric hydrolysable tannins

Radebe

Rode

Pizzi

et al. 2012

J of Applied Polymer Sci

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High molar mass wood tannin extracts are complex mixtures that are distributed in both molar mass and chemical composition. Hydrolysable tannins from tara, Turkey gall, and chestnut woods were analyzed and compared using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Although MALDI-TOF MS reveals the oligomer structure of the tannins, this method cannot distinguish between isomers with isobaric masses and, therefore, ambiguous structural assignments were made in a number of cases. To determine the actual microstructures present, MALDI-TOF-CID (collision induced dissociation) experiments were conducted. MALDI-TOF-CID enables monomer sequence determination and positive assignments of isobaric structures can be made

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Section: Introductionmentioning

confidence: 99%

“…Recently, ion trap mass spectrometry has been used for the analysis of tannins. Corke and coworkers22,23 identified phenolic antioxidants in Chinese roses and galls while Zhang and Lin analyzed condensed tannins in Pacific oak leaves 10…”

Section: Introductionmentioning

confidence: 99%

MALDI‐TOF‐CID for the microstructure elucidation of polymeric hydrolysable tannins

Radebe

Rode

Pizzi

et al. 2012

J of Applied Polymer Sci

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“…Tannins have received considerable attention in the fields of nutrition, health and medicine, largely due to their physiological activity such as antioxidant activity, anti-microbial effects, and anti-inflammatory properties. Tannins are powerful antioxidants often characterized by reducing power and free radical scavenging activities [33]. In addition, the astringent taste of L. aspera may be attributed to the high concentrations of tannins which prevent it from herbivory.…”

Section: Resultsmentioning

confidence: 99%

Pharmacognostical and Physicochemical Evaluation of the Plant Leucas Aspera

Kripa

Sangeetha

Chamundeeswari

2016

Asian J Pharm Clin Res

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Objective: Leucas aspera (Willd.) Linn. is a herbaceous annual distributed throughout India from the Himalayas down to Ceylon. This study was aimed to investigate the pharmacognostical, phytochemical characteristics, and heavy metal content of the aerial parts of L. aspera. Methods:The aerial parts of the plant were subjected to macroscopical and microscopical characterization, followed by physicochemical evaluations using standardized procedures. Atomic absorption spectroscopy (AAS) of the dried plant powder was done to determine the metal and mineral content. Preliminary phytochemical screening and metabolite analysis of the hydroalcoholic extract of L. aspera (HAELA) were also performed to analyze the various phytoconstituents. Results:In this study, the microscopic characteristics of L. aspera were found to be consistent with earlier reports. The total ash value was relatively high (11.5% w/w). Alcohol soluble and water-soluble extractive values were found to be 6.5% and 9%, respectively. AAS indicated the plant to contain insignificant quantities of heavy metals and considerable amounts of copper, sodium, potassium, and iron. Phytochemical analysis revealed the presence of alkaloids, flavonoids, glycosides, lignins, phenols, saponins, sterols, and tannins in HAELA. Conclusion:The pharmacognosy -anatomical, physicochemical, heavy metal characteristics, and the preliminary phytochemical studies of the aerial parts of L. aspera have revealed the presence of phytoconstituents such as triterpenoids, phenolic compounds, flavonoids, and glycosides; insignificant quantities of heavy metals and significant content of phenolics and tannins in the plant.

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“…The signals of the 13 C NMR spectrum of the purified EASPA fraction were assigned using those reported in the literature for other proanthocyanidins. 19,20 The spectrum shows characteristic Thus, the monomeric unit of procyanidin can be specifically identified as epicatechin. The presence of (epi)gallocatechin was also confirmed by 13 C NMR spectroscopic studies.…”

Section: Resultsmentioning

confidence: 99%

Untitled

2016

IJPSR

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ABSTRACT:The preliminary phytochemical analysis of the aqueous ethanolic extract of the bark of Thespesia populnea (L.) revealed the presence of alkaloids, unsaturated sterols, triterpenes, flavonoids and the absence of anthraquinones. The froth test indicated the presence of saponins. Most importantly this study revealed the presence of proanthocyanidins which has not been reported before. In the present study, ethyl acetate and aqueous soluble proanthocyanidin fractions (EASPA and AQSPA respectively) were extracted and purified by chromatography on Sephadex LH-20. Prussian blue test and acid catalyzed cleavage test revealed that proanthocyanidins have been successfully separated from non-proanthocyanidin phenolics. The yields of purified EASPA and AQSPA fractions were 0.081% and 0.72% (by weight) of the fresh bark. Acid catalyzed cleavage followed by TLC studies of both EASPA and AQSPA fractions showed the presence of two anthocyanidins, cyanidin and delphinidin, suggesting that they are composed of (epi)catechin and (epi)gallocatechin units with (epi)catechin being more abundant compared to the other. This was further confirmed by 13 C NMR spectroscopic studies. In addition, 13C NMR spectroscopic studies revealed (epi)catechin to be the epi-isomer. The preliminary antioxidant activity of purified EASPA and AQSPA fractions was determined using the DPPH assay. The IC 50 values of EASPA and AQSPA (0.0725 mg/mL and 0.0781 mg/mL respectively) were clearly lower than that of ascorbic acid (0.125 mg/mL), which is an established standard used in antioxidant studies. Thus, proanthocyanidin samples appear to possess better antioxidant capacity than ascorbic acid. In addition, EASPA and AQSPA fractions were shown to mediate cytotoxic activity against MCF 7 cells through SRB assay.

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HPLC, NMR and MALDI-TOF MS Analysis of Condensed Tannins from Lithocarpus glaber Leaves with Potent Free Radical Scavenging Activity

Cited by 59 publications

References 32 publications

MALDI‐TOF‐CID for the microstructure elucidation of polymeric hydrolysable tannins

MALDI‐TOF‐CID for the microstructure elucidation of polymeric hydrolysable tannins

Pharmacognostical and Physicochemical Evaluation of the Plant Leucas Aspera

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