HPLC/MS-TOF Analysis of Surface Resins from Three Poplar Clones Grown in Serbia

Abstract

Background and Purpose: Poplar clones grown in Serbia are fast growing tree species important for many different purposes in forestry and industry. In this study chemical content of the surface resins of three poplar clones grown in Serbia-M1, B229 and PE 19/66 was analyzed, aiming at their potential usage as a source of natural products important for pharmacy and chemotaxonomy. Materials and Methods: Using HPLC/MS-TOF we gained the first information on chemical compounds which comprise of resins on terminal twigs cuttings of commonly grown poplar clones. Provided from the nursery of the Institute of Lowland Forestry and Environment (Serbia), terminal twigs cuttings with leaves of different development stage from two year old seedlings of M1 poplar clone (Populus euramericana L.), PE 19/66 clone and B229 clone (both belonging to Populus deltoides) were sampled. The washing of the surface resins from terminal twigs cuttings of every sample was done with methylene-chloride until the samples were prepared for HPLC/MS-TOF analysis. Results: Out of 38 different compounds which were identified, M1 clone qualitatively differed for 14 compounds as compared to two other clones. Generally, the results showed that the composition of the resins consisted of different phenolic acids, phenolic esters, flavonoids and other contents. Conclusion: These three poplar clones are potent producers of pharmacologically and chemotaxonomically potent compounds in forest ecosystems, especially M1 clone.

Profile of Polyphenolic and Essential Oil Composition of Polish Propolis, Black Poplar and Aspens Buds

“…However, some lower concentration ions in some peaks show similarity to salicin (M/Z for ESI-NEG = 285.1) and other salicylate-like compounds. This result was probably due to low concentration of salicin-like substances in poplar buds, extraction procedure and HPLC division condition [ 23 , 24 ]. It is also noteworthy that salicylic compounds characteristic for aspens (e.g., tremuloidin or tremulacin) were not observed [ 25 ].…”

Extraction, Antioxidant Capacity, 5-Lipoxygenase Inhibition, and Phytochemical Composition of Propolis from Eastern Canada

“…Following the initial LC-MS/MS method development with the seven standards, an ultrasound-assisted ethanol extract was tested to tentatively identify other precursor ions from other abundant compounds. Based on this analysis and a comparison to several other published lists of propolis components [33][34][35][36][37][38][39], seventeen more standards were purchased from Sigma-Aldrich (Mississauga, ON, Canada). All twenty-four standards (including gallic acid) were tested by LC-MS/MS on the quadrupole-Orbitrap and by infusion on a linear quadrupole ion trap mass spectrometer (LTQ-XL, Thermo-Fisher Scientific, San Jose, CA, USA).…”

“…The chemical profiles of several propolis extracts were analyzed by gradient microscale liquid chromatography (Ultimate 3000, Thermo-Fisher Scientific, San Jose, CA, USA) coupled to a high-resolution/accurate mass quadrupole-Orbitrap mass spectrometer using a pneumatically-assisted electrospray ionization ion source. Electrospray ionization has previously been used to "fingerprint" propolis by mass spectrometry using negative ion mode [34]. Complex extract samples were efficiently fractionated using on-line gradient liquid chromatography with a 10 cm long, 1.0 mm diameter reversed-phase (Altima C18) column with 3 µm particles (VWR, Mississauga, ON, Canada).…”

Metabolite profiling of the medicinal herb Phyllanthus niruri L. under drought stress

“…The chemical characteristics of the cuticular wax and the increase in wax are the main determinants of plant cuticle permeability. Cuticle synthesis is influenced by genetics and the environment under certain conditions [17]. According to low-water conditions, plants synthesize various lipid substances for detoxification and protection of pathogens, such as heneicosane and substances for drought tolerance mechanisms [18].…”