HPLC-MS-based untargeted metabolomic analysis of differential plasma metabolites and their associated metabolic pathways in reproductively anosmic black porgy, Acanthopagrus schlegelii

Xing, Shu; Li, Xian; Jia, Chaofeng; Li, Jun; Chen, Shuyin; Gao, Bo; Liang, Wenke; Zhang, Libin

doi:10.1016/j.cbd.2023.101071

Cited by 6 publications

(4 citation statements)

References 64 publications

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“…To verify the reliability of the model, 200 iterative substitution tests were conducted. The intersection of the Q 2 regression line with the Y-axis was on the negative semi-axis, further indicating that the PLS-DA model was stable and reliable (Figure 4B) [31]. As shown in Table 1, the common pathway of the three cutting styles was the phenylpropanoid biosynthesis pathway; however, the metabolites involved in the different cutting styles were different, and coniferyl aldehyde and eugenol were unique to the shredding style.…”

Section: Metabolomic Changesmentioning

confidence: 78%

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Effects of Different Cutting Styles on Physiological Properties in Fresh-Cut Carrots

Zhou,

Ma,

Zhang

et al. 2024

Plants

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With the internationalization of Chinese culture, ready-to-cook Chinese food has become popular. Vegetables in Chinese preparations are usually cut into slices, cubes, and shreds. Carrots, as a typical Chinese side dish, were selected as the model in this work. The polyphenol content, antioxidant capacity, O2−, hydrogen peroxide, malondialdehyde, lignin, antioxidant enzymes, and other enzymes activities were analyzed. The results indicated that these parameters were insignificantly different between three cutting styles. Therefore, metabolomics is further employed. Pathway enrichment indicated that glyceollin II and 6″-malonylgenistin were metabolites particularly expressed in the isoflavonoid biosynthesis pathway; (+)-gallocatechin, trans-chlorogenic acid, and (−)-epiafzelechin were specifically identified in the flavonoid biosynthesis pathway after slicing; and shredding caused the expression of coniferyl aldehyde and eugenol, which were specifically expressed in the phenylpropanoid biosynthesis pathway. These results indicate that different cutting styles do not change the physiological indicators of carrots but induce the expression of specific metabolites.

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Section: Metabolomic Changesmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Effects of Different Cutting Styles on Physiological Properties in Fresh-Cut Carrots

Zhou,

Ma,

Zhang

et al. 2024

Plants

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“…Current research indicates that CUMS stimulation reduced Enterorhabdus abundance, which was reversed by venlafaxine treatment. So Enterorhabdus are the key bacteria responsible for venlafaxine-ameliorated depression in mice ( Shen et al, 2023 ). In a rat model of ACTH-induced depression, chlorogenic acid exerted anti-depressive effects by increasing the relative abundance of Bifidobacterium and reducing the relative abundance of Desulfovibrio ( Song et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Investigating the antidepressant effect of Ziyan green tea on chronic unpredictable mild stress mice through fecal metabolomics

Jia,

Tang,

Zou

et al. 2023

Front. Microbiol.

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IntroductionSome studies have shown the effectiveness of tea in reducing depression. Gut flora dysfunction is strongly associated with depression. The mechanism by which Ziyan green tea ameliorates depression is not clear.MethodsIn this study, we examined the impact of Ziyan green tea on mice exhibiting symptoms similar to depression. We specifically focused on the role of intestinal flora and its metabolites. We first established a chronic unpredictable mild stress (CUMS) mouse model to induce depressive symptoms and conducted behavioural tests, biochemical tests, and pathological tissue analysis. We also investigated gut microbiota changes by 16S rRNA sequencing and measured faecal metabolites in mice using UHPLC-MS/MS.ResultsThe results showed that Ziyan green tea intervention improved depression-like behaviour, neurobiochemical factors, and reduced levels of pro-inflammatory factors in CUMS mice. Spearman’s correlation analysis showed that different microbial communities (Corynebacterium, Faecalibaculum, Enterorhabdus, Desulfovibrio) correlation with differential metabolites (Cholic acid, Deoxycholic acid, etc.) and depression-related biochemical indicators (5-HT, DA, BDNF, IL-6, and TNF-α).DiscussionIn conclusion, our findings suggest that both low and high-dose interventions of Ziyan green tea have positive preventive effects on CUMS mice without dose dependence, partly because they mainly affect intestinal Purine Metabolism, Bile Acid Biosynthesis and Cysteine Metabolism in CUMS mice, thus stimulating brain 5-HT, DA and BDNF, and decreasing the inflammatory factors IL-6, TNF-α, activate the composition of intestinal flora, improve the intestinal flora environment and thus promote the production of intestinal metabolites, which can be used for depression treatment. It is suggested that Ziyan green tea may achieve an antidepressant effect through the gut-microbiota-brain axis.

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“…Consequently, distinguishing them from the metabolites in vivo becomes challenging [ [12] , [13] , [14] ], thereby significantly impacting the accuracy of medicinal material metabolism results [ 15 ], and leading to an unclear understanding of the pharmacodynamic material basis in vivo [ 16 , 17 ]. Traditional studies on the in vivo metabolism of medicinal have typically relied on analyzing the metabolic pathways of representative components and predicting metabolites for similar components to evaluate the efficacy of traditional Chinese medicine metabolism, or comparing chemical fingerprints of plant materials with metabolite profiles to identify in vivo metabolites [ [18] , [19] , [20] ]. However, these traditional methods involve complex data processing, large sample size, and procedures.…”

Section: Introductionmentioning

confidence: 99%

The mysteries of pharmacokinetics and in vivo metabolism of Oroxylum indicum (L.) Kurz: A new perspective from MSOP method

Zhang,

Wang

et al. 2024

Heliyon

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HPLC-MS-based untargeted metabolomic analysis of differential plasma metabolites and their associated metabolic pathways in reproductively anosmic black porgy, Acanthopagrus schlegelii

Cited by 6 publications

References 64 publications

Effects of Different Cutting Styles on Physiological Properties in Fresh-Cut Carrots

Effects of Different Cutting Styles on Physiological Properties in Fresh-Cut Carrots

Investigating the antidepressant effect of Ziyan green tea on chronic unpredictable mild stress mice through fecal metabolomics

The mysteries of pharmacokinetics and in vivo metabolism of Oroxylum indicum (L.) Kurz: A new perspective from MSOP method

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