HPLC/MS Application to Anthocyanins of Vitis vinifera L.

Baldi, A.; Romani, Annalisa; Mulinacci, Nádia; Vincieri, Franco Francesco; Casetta, Bruno

doi:10.1021/jf00056a027

Cited by 139 publications

(107 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Application of modern mass spectrometry techniques, such as liquid chromatography mass spectrometry (LC-MS) and matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), became the most sophisticated techniques for anthocyanin analyses in grape and wine. Mass spectrometry technique is applicable and effective for studies of glycoside compounds, allowing characterization of the aglycone and sugar moiety (Pérez-Magariño et al 1999, de Villers et al 2004, Monagas et al 2005Baldi et al 1995, Cameira-dos-Santos et al 1996, Wang and Sporns 1999, Revilla and González-San José 2001, Núñez et al 2004). …”

Section: Introductionmentioning

confidence: 99%

Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

et al. 2010

View full text Add to dashboard Cite

Matrix-assisted laser desorption/ionization timeof-flight mass spectrometry (MALDI-TOF-MS) as a new valuable technique has been used for qualitative screening of anthocyanins in wine and grape samples from Vranec and Merlot variety. For that purpose, different MALDI matrices have been tested, such as α-cyano-4-hydroxycinnamic acid, sinapic acid, 2,5-dihydroxybenzoic acid (2,5-DHB), and C 70 fullerene. It was observed that 2,5-DHB was superior with respect to all the matrices used in the study. Furthermore, fullerene was, for the first time, tested and used as a possible matrix for MALDI-TOF-MS identification of anthocyanins in wine and grape samples. The results obtained showed identification of aglycone forms of anthocyanins only, since the higher laser energy applied for ionization of the fullerene molecules led to great deal of fragmentation of the sugar moiety from glucosides, acetylglucoside, and p-coumaroylglucoside groups from the corresponding anthocyanin molecules. Additionally, liquid chromatography/electrospray ionization mass spectrometry has also been applied for anthocyanin characterization.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Various ionisation methods such as electrospray ionisation (ESI) [74,[89][90][91][92], atmospheric pressure chemical ionisation (APCI) [77], continuous flow fast atom bombardment (CF-FAB) [79] and matrix assisted laser desorption ionisation (MALDI) [72] have been used. Quadrupole mass analysers are most widely applied, due to their robustness and low cost.…”

Section: Detection Methodsmentioning

confidence: 99%

“…Especially in the last 2 decades, mass spectrometry (MS) has also found extensive application in anthocyanin analysis, with ionisation methods such as matrix assisted laser desorption ionisation (MALDI) [72,73], electrospray ionisation (ESI) [74,75], atmospheric pressure chemical ionisation (APCI) [76,77] [78,79] having been applied. The main focus of the next section will therefore be on LC and LC-MS analysis of anthocyanins.…”

Section: Analysis Of Anthocyaninsmentioning

confidence: 99%

Comprehensive two-dimensional liquid chromatographic analysis of anthocyanins

Willemse¹,

Stander²,

Tredoux³

et al. 2014

Journal of Chromatography A

View full text Add to dashboard Cite

“…Baldi et al proposed a complex fractionation method for grape by using an Extrelut 20 cartridge to remove lipophilic and polyphenolic compounds with hexane and ethylacetate followed by adsorption of the sample on to a C18 cartridge to eliminate hydrophilic molecules e.g. sugars and organic acids ( Baldi, Romani, Mulinacci, Vincieri & Casetta, 1995). This study describes a method for extraction, isolation and purification of anthocyanins from fruits of blueberry and cranberry and rose leaves.…”

Section: Introductionmentioning

confidence: 99%

Effect of Mobile Phase Variation and Purification on Chromatogram View by Using Fruits and Rose Extracts and HPLC Method

Çetin¹,

Turak²

2016

IJC

View full text Add to dashboard Cite

In this study extracts obtained from fruits of bluebery and cranberry and rose leaves were chromatografied by using mobile phase variation and DAD detector. The chromatograms were evaluated under different mobile phase systems such as isocratic and gradient elutions following purification procedure. The best separation conditions of pigments of anthocyanin in extracts and efficiency of purification procedure were searched by using mobile phases in the elution of isocratic and gradient modes. The chromatogram views have shown existence of anthocyanin constituents in the all fruits and rose extracts involving separation and qualitation. The effect of purification for separation and removal of pigments has been investigated.

show abstract

HPLC/MS Application to Anthocyanins of Vitis vinifera L.

Cited by 139 publications

References 4 publications

Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

Comprehensive two-dimensional liquid chromatographic analysis of anthocyanins

Effect of Mobile Phase Variation and Purification on Chromatogram View by Using Fruits and Rose Extracts and HPLC Method

Contact Info

Product

Resources

About