Abstract:Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5µm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column tem… Show more
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