HPLC Method for Simultaneous Estimation of Paclitaxel and Baicalein: Pharmaceutical and Pharmacokinetic Applications

Abstract

Aim: A novel HPLC method was developed and validated for the simultaneous estimation of paclitaxel (PTX) and baicalein (BAC). Materials & methods: The analytes were resolved in a C18 column using the aqueous solution of formic acid (0.10% v/v) and MeOH (30:70 v/v). Results: The developed method was found to be linear over the concentration ranges 0.039–10 μg/ml and 0.019–10 μg/ml for PTX and BAC, respectively. The lower limits of quantification obtained were 0.042 μg/ml and 0.361 μg/ml for PTX and BAC, respectively. Conclusion: The developed method was found to be precise and accurate as per the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use guidelines, for simultaneous estimation of PTX and BAC, having an application in formulation development and bioanalytical studies.

Liquid Chromatography Dependent Stability Indicating Methodology: Development and Authentication for Formulations of Capsule Type Containing Tegafur, Gimeracil, and Oteracil

“…The fundamental benefit of liquid chromatography is its extensive use in academics, education, and routine development over decades, leading to generally recognized and extensively implemented ways of method innovation, method improvement, and problem-solving. Given that liquid chromatography methodologies are the preferred analytical strategy, many researchers have worked to develop a variety of liquid chromatography techniques for the simultaneous estimate of various active elements in multi-constituent medications [13][14][15][16][17][18][19][20][21].…”