HPLC method for determination of fluorescence derivatives of cortisol, cortisone and their tetrahydro- and allo-tetrahydro-metabolites in biological fluids

Główka, Franciszek; Kosicka, Katarzyna; Karaźniewicz−Łada, Marta

doi:10.1016/j.jchromb.2009.11.016

Cited by 19 publications

(17 citation statements)

References 45 publications

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“…That is why THE and aTHE frequently are determined in human clinical practice as a combination. More often, only THE is considered as the reduced metabolite of E while the level of aTHE is ignored (Główka et al 2010). The reason for such simplification may be the much lower level of aTHE in comparison to THE in human urine (Vierhapper 2000).…”

Section: Loss Of Formaldehyde [M -Ch 2 O-h]mentioning

confidence: 99%

HPLC-ESI-MS/MS assessment of the tetrahydro-metabolites of cortisol and cortisone in bovine urine: promising markers of dexamethasone and prednisolone treatment

Chiesa

Panseri

Pavlović

et al. 2016

Food Additives & Contaminants: Part A

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The effects of long-term administration of low doses of dexamethasone (DX) and prednisolone (PL) on the metabolism of endogenous corticosteroids were investigated in veal calves. In addition to cortisol (F) and cortisone (E), whose interconversion is regulated by 11β-hydroxysteroid dehydrogenases (11βHSDs), special attention was paid to tetrahydrocortisol (THF), allo-tetrahydrocortisol (aTHF), tetrahydrocortisone (THE) and allo-tetrahydrocortisone (aTHE), which are produced from F and E by catalytic activity of 5α and 5β-reductases. A specifically developed HPLC-ESI-MS/MS method achieved the complete chromatographic separation of two pairs of diastereoisomers (THF/aTHF and THE/aTHE), which, with appropriate mass fragmentation patterns, provided an unambiguous conformation. The method was linear (r 2 > 0.9905; 0.5-25 ng ml . Recoveries were in range 75-114%, while matrix effects were minimal. The experimental study was carried out on three groups of male Friesian veal calves: group PL (n = 6, PL acetate 15 mg day -1 p.o. for 31 days); group DX (n = 5, 5 mg of estradiol (E2) i.m., weekly, and 0.4 mg day -1 of DX p.o. for 31 days) and a control group (n = 8). Urine was collected before, during (twice) and at the end of treatment. During PL administration, the tetrahydro-metabolite levels decreased gradually and remained low after the suspension of treatment. DX reduced urinary THF that persisted after the treatment, while THE levels decreased during the experiment, but rebounded substantially after the DX was withdrawn. Both DX and PL significantly interfered with the production of F and E, leading to their complete depletion. Taken together, the results demonstrate the influence of DX and PL administration on 11βHSD activity and their impact on dysfunction of the 5-reductase pathway. In conclusion, profiling tetrahydrometabolites of F and E might serve as an alternative, indirect but reliable, non-invasive procedure for assessing the impact of synthetic glucocorticosteroids administration. ARTICLE HISTORY

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Section: Loss Of Formaldehyde [M -Ch 2 O-h]mentioning

confidence: 99%

HPLC-ESI-MS/MS assessment of the tetrahydro-metabolites of cortisol and cortisone in bovine urine: promising markers of dexamethasone and prednisolone treatment

Chiesa

Panseri

Pavlović

et al. 2016

Food Additives & Contaminants: Part A

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“…Levels of plasma and urinary GCs were determined using previously described HPLC-FLD method. 16 Concentrations of F and E in plasma, as well as amounts of UFF, UFE, THF, allo-THF, THE þ allo-THE in urine were assessed. The total levels of THF, allo-THF, THE and allo-THE were determined after enzymatic hydrolysis with the use of b-glucuronidase under the previously presented conditions.…”

Section: Steroid Analysismentioning

confidence: 99%

“…The total levels of THF, allo-THF, THE and allo-THE were determined after enzymatic hydrolysis with the use of b-glucuronidase under the previously presented conditions. 16 Subsequently, specific parameters were calculated to estimate 11b-HSD2 activity: plasma F/E, UFF/UFE in urine and (THF þ allo-THF)/(THE þ allo-THE) in urine. The following reference ranges were accepted:p4.00 for F/E in plasma; p0.600 for UFF/UFE in urine andp1.40 for (THF þ allo-THF)/(THE þ allo-THE) in urine.…”

Section: Steroid Analysismentioning

confidence: 99%

11β-Hydroxysteroid dehydrogenase type 2 in hypertension: comparison of phenotype and genotype analysis

et al. 2013

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11β-Hydroxysteroid dehydrogenase type 2 (11β-HSD2) catalyzes the inactivation of cortisol (F) to cortisone (E) in aldosterone target tissues, thereby protects mineralocorticoid receptor from F. Failure of 11β-HSD2 function is the basis of apparent mineralocorticoid excess, and its mild disturbances are suggested to lead to hypertension. The aim of the study was to analyze the 11β-HSD2 activity in hypertensives and healthy volunteers. Glucocorticoids (GCs) profile was estimated to verify whether the disorders of GCs balance may be involved in essential hypertension etiology. Exons and short introns of HSD11B2 were sequenced to evaluate existing mutations and their potential implications in the disease. The identified polymorphisms were assessed in case-control study to determine their relevance to hypertension. No significant differences in values of plasma F/E and UFF/UFE (urinary free F to free E) were observed between hypertensives and controls. The value of (THF+allo-THF)/(THE+allo-THE) (urinary tetrahydro-metabolites of F to tetrahydro-metabolites of E) in hypertensives was higher than in normotensives. Logistic regression demonstrated that the increase of one unit of (THF+allo-THF)/(THE+allo-THE) value increases the risk of hypertension over 11-fold. Genotyping indicated no hypertension related mutations in the coding region and short introns of HSD11B2.

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“…Unfortunately, the intensity of their fluorescence is not strong because of the short emission wavelength (310 nm) [8]. G ówka et al [132] proposed analysis of steroids in plasma and urine samples. Steroids, being generally non-fluorescent reagents due to the lack of active functional groups (only the 21-hydroxyl group converts in fluorescent derivatization) are difficult to convert to fluorescent derivatives.…”

Section: Fluorescence and Chemiluminescence Detectionmentioning

confidence: 99%

“…Steroids, being generally non-fluorescent reagents due to the lack of active functional groups (only the 21-hydroxyl group converts in fluorescent derivatization) are difficult to convert to fluorescent derivatives. However, the use of 9-anthroylnitrile (9-AN) [132] triggered cortisol conversion into its fluorescent derivative. Nozaki [133] proposed separation of the serum sample cortisol and prednisolone, both nonfluorescent, using pre-column sulphuric acid-ethanol fluorescence derivatisation, which enables obtaining the detection limit of 3 ng/ml for both steroids.…”

Section: Fluorescence and Chemiluminescence Detectionmentioning

confidence: 99%

Plasma Steroid Level Measured Using Modern Separation Techniques as Biomarkers in Biological Diagnostics

Konieczna¹,

Bączek

2010

CPA

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During the last decade there has been an increased focus on the application of steroids as biomarkers in biomedical practice. The analysis of steroid hormones in biological samples of plasma or serum is currently routinely used in clinical diagnosis being an essential source of information on not only metabolic pathways, but also disorders of the metabolism. More importantly, the steroid hormones may reduce cancer development on the endocrinal basis, reveal abuse of anabolic substances, or even depression incidences. In the case of biomedical research, quantitative determination of steroids in serum or plasma creates an opportunity to diagnose diseases efficiently in their early stages and to monitor a patient for a possible recurrence of a disease after therapy. Therefore, an accurate measurement of steroids in the plasma or serum has become important for the contemporary medicine, even if troublesome especially in view of their low concentration in biological samples.The most common methods of steroid quantification in clinical practice nowadays include immunoassays such as radioimmunoassay or enzyme immunoassay. However, the main disadvantage of the immunoassay techniques consists in cross reactivity of the antibodies used in the assay with the related hormones. On the other hand, there is a number of applications where the analysis is performed with the use of modern separation techniques.The aim of this review is to present the development and applicability of the various analytical methods, all based on separation techniques and used for determination of steroids in the clinical laboratory, taking into account the recent progress in those areas. The review shows the advantages of high-performance liquid chromatography applied to determine non-volatile and thermally labile steroids and steroid conjugates. It also demonstrates that the currently used mass spectrometry offers practically useful structural information on the composition of steroids. A number of techniques and methods are compared and critically discussed pondering finally their specific analytical requirements like sensitivity, specificity, simplicity, limit of detection, and quantification. Nonetheless, plasma or serum sampling seems to remain one of the most convenient methods for population screening purposes.

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HPLC method for determination of fluorescence derivatives of cortisol, cortisone and their tetrahydro- and allo-tetrahydro-metabolites in biological fluids

Cited by 19 publications

References 45 publications

HPLC-ESI-MS/MS assessment of the tetrahydro-metabolites of cortisol and cortisone in bovine urine: promising markers of dexamethasone and prednisolone treatment

HPLC-ESI-MS/MS assessment of the tetrahydro-metabolites of cortisol and cortisone in bovine urine: promising markers of dexamethasone and prednisolone treatment

11β-Hydroxysteroid dehydrogenase type 2 in hypertension: comparison of phenotype and genotype analysis

Plasma Steroid Level Measured Using Modern Separation Techniques as Biomarkers in Biological Diagnostics

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