HPLC Method for Detection of Ergotamine, Ergosine, and Ergine after Intravenous Injection of a Single Dose

Moubarak, Ali S.; Piper, E. L.; Johnson, Z. B.; Flieger, Miroslav

doi:10.1021/jf950425i

Cited by 30 publications

(17 citation statements)

References 12 publications

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“…LC-FLD was used to measure ergosine, ergotamine and ergine in bovine serum based on C18 SPE trapping and clean-up [ 91 ]. Recovery of ergosine was 95% to 98% at the detection limit (0.5 ng/mL) and up to 24 ng/mL.…”

Section: Specific Applicationsmentioning

confidence: 99%

Analysis of Ergot Alkaloids

Crews

2015

Toxins

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The principles and application of established and newer methods for the quantitative and semi-quantitative determination of ergot alkaloids in food, feed, plant materials and animal tissues are reviewed. The techniques of sampling, extraction, clean-up, detection, quantification and validation are described. The major procedures for ergot alkaloid analysis comprise liquid chromatography with tandem mass spectrometry (LC-MS/MS) and liquid chromatography with fluorescence detection (LC-FLD). Other methods based on immunoassays are under development and variations of these and minor techniques are available for specific purposes.

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Section: Specific Applicationsmentioning

confidence: 99%

Analysis of Ergot Alkaloids

Crews

2015

Toxins

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“…At Mount Vernon, sampling occurred on May 6, June 2, June 18, and October 20, 1999, and on June 7, 2000. Forage samples were lyophilized and ergovaline concentrations were determined by HPLC and expressed as the sum of ergovaline and its epimer ergovalinine (Moubarak et al, 1996). Forage loline levels were also determined from collection of each pasture on May 24, 1999 and May 31, 2000 at Fayetteville.…”

Section: Animal and Pasture Treatment For Both Locationsmentioning

confidence: 99%

Growth rate and physiology of steers grazing tall fescue inoculated with novel endophytes

Nihsen

Piper

West

et al. 2004

Journal of Animal Science

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Cattle grazing tall fescue (Festuca arundinacea Schreb.) often develop fescue toxicosis. This condition is thought to be caused by ergot alkaloids produced by the endophyte Neotyphodium coenophialum. Endophytes from wild tall fescue plants, which do not produce ergot alkaloids, were transferred into the endophyte-free tall fescue germplasm, HiMag. The novel associations also lacked the ability to produce ergot alkaloids. Our objective was to determine whether cattle grazing these novel endophyte associations showed signs of fescue toxicosis. At the Fayetteville, Arkansas location, tester steers (n = 72) were assigned to one of four pasture treatments: endophyte-free HiMag tall fescue (HiMag-); 'Kentucky-31' tall fescue infected with its native, toxic endophyte (KY+); and two novel endophyte-infected tall fescue associations, HiMag4 and HiMag9. At the Mount Vernon, Missouri location, steers (n = 54) were used to test three of the four cultivars (HiMag9 was not tested). Ergot alkaloid concentrations in the forage of HiMag4 and HiMag9 were low or undetectable. Respiration rate, rectal temperature, ADG, and hair scores were measured during the grazing period. Blood was collected via jugular venipuncture and used for prolactin, aspartate aminotransferase, alkaline phosphatase (ALP), lactate dehydrogenase (LDH), cholesterol, triglyceride, and creatinine analysis. Weight gains by steers grazing HiMag4 and HiMag9 did not differ from those of steers grazing HiMag-, but were greater than gains (P < 0.05) by steers on the KY+ treatment. Steers grazing KY+ had higher (P < 0.05) respiration rates, rectal temperatures, and hair scores than did steers grazing novel endophyte and HiMag- pastures. Prolactin, ALP, cholesterol, LDH, and triglycerides all were suppressed (P < 0.05) in steers grazing KY+ compared with steers grazing novel endophyte and HiMag- pastures. Steers grazing the novel endophyte tall fescues did not suffer from the decreased weight gains and toxicities associated with fescue toxicosis, resulting in enhanced animal production.

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“…Pastures were sampled on June 18 to measure ergovaline, an ergopeptine used as an indicator of the potential toxicity of consumed forage (Garner et al, 1993;Sleper and West, 1996). Single, whole tillers from 25 randomly chosen plants were collected and combined for each pasture and stored frozen for subsequent determination of ergovaline concentration following procedures described by Moubarak et al (1996).…”

Section: Pasture Phasementioning

confidence: 99%

Influence of protein supplementation and implant status on alleviating fescue toxicosis.

Aiken

Piper²,

Miesner³

2001

Journal of Animal Science

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Heat stress is a major problem in transporting stocker calves with symptoms of fescue toxicosis. Removing calves from tall fescue pastures and offering diets devoid of endophyte-infected tall fescue could reduce the severity of toxicosis and precondition calves for transport to the feedlot. In the present experiment, a pasture phase was used to condition yearling steers to grazing tall fescue and induce symptoms of fescue toxicosis, and a pen phase followed to determine effects of implanting at the start of grazing and protein supplementation (hay only vs hay plus supplement) on shortterm changes in rectal temperature and serum prolactin concentration. Neither implant status nor protein supplementation affected (P > 0.10) white blood cell count or rectal temperature. White blood cell counts at

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HPLC Method for Detection of Ergotamine, Ergosine, and Ergine after Intravenous Injection of a Single Dose

Cited by 30 publications

References 12 publications

Analysis of Ergot Alkaloids

Analysis of Ergot Alkaloids

Growth rate and physiology of steers grazing tall fescue inoculated with novel endophytes

Influence of protein supplementation and implant status on alleviating fescue toxicosis.

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