HPLC Method for Analysis of Secoisolariciresinol Diglucoside in Flaxseeds

Johnsson, Pernilla; Kamal‐Eldin, Afaf; Lundgren, L.; Åman, Per

doi:10.1021/jf0005871

Cited by 191 publications

(131 citation statements)

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“…The content of secoisolariciresinol diglucoside in flaxseed has been reported to vary from 6.1 to 25.9 mg g (1 (Johnsson et al 2000;Eliasson et al 2003). Lignans are classified as phytoestrogens because of their structural similarities with natural and synthetic estrogens and antiestrogens, but they are also able to compete with endogenous estradiol for estrogen receptors.…”

Section: Flaxseed Compositionmentioning

confidence: 99%

Review: Feed intake, milk production and milk composition of dairy cows fed flaxseed

Petit¹

2010

Can. J. Anim. Sci.

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, milk production and milk composition of dairy cows fed flaxseed. Can. J. Anim. Sci. 90: 115Á127. Flaxseed contains approximately 55% of total fatty acids of the oil as a-linolenic acid and is rich in lignans, which are strong antioxidants. Diets rich in omega-3 fatty acids and antioxidants are known to have beneficial effects on human health such as a decrease in the incidence of cancer, cardiovascular diseases, hypertension, and arthritis. Flaxseed could then be an interesting natural feed to consider for changing milk composition. Cyanogenic glycosides (linustatin and neolinustatin) are present in flaxseed, but the concentration of hydrocyanic acid is very low in milk and ruminal fluid of cows fed flaxseed products. In general, feeding up to 15% of the total dry matter as whole flaxseed has a limited effect on dry matter intake. Heat treatments such as micronization and extrusion have no effect on dry matter intake and the effect of formaldehyde treatment on feed intake is unclear. The effects of flaxseed supplementation on milk production of dairy cows in the early stage of lactation have been neutral. Diet supplementation with whole flaxseed has had no effect on milk yield and composition of dairy cows in the mid or late stages of lactation. Physical processing of flaxseed increased milk production although heat treatment did not. Results on the effect of flaxseed processing on overall milk fat concentration have been controversial, but heat and formaldehyde treatments had no effect. Flaxseed supplementation had no effect on milk fat and protein concentrations, and processing of flaxseed had little effect. The extent of change in the concentration of fatty acids in milk is generally proportional to the level of inclusion of flaxseed in the diet. In conclusion, feeding flaxseed does not affect milk production or composition in the large majority of studies, but its long-term effects on health of cows and productivity still need to be determined.

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Section: Flaxseed Compositionmentioning

confidence: 99%

Review: Feed intake, milk production and milk composition of dairy cows fed flaxseed

Petit¹

2010

Can. J. Anim. Sci.

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“…Yuan et al (2008) found SDG in DFF in the range of 6-29 mg g −1 and Li et al (2008) reported a SDG content of 15.4 mg g −1 in flaxseed. In addition, Johnsson et al (2000) obtained SDG in the range of 11.7-24.1 mg g −1 in DFF and 6.1-13.3 mg g −1 in whole flaxseed from cultivars of Denmark and Sweden. Hao and Beta (2012) reported that the SDG content in flaxseed hulls varied from 16.4 to 33.9 mg g −1 .…”

Section: Sdg Content In Flaxseed Cultivarsmentioning

confidence: 94%

“…DFF was prepared by the Soxhlet method for 2 h at 70°C with nhexane, as described by Johnsson et al (2000) with some modifications. DFF (5 g) was mixed with 100 mL of 0.1 mmol L −1 sodium methoxide and sonicated for 30 min.…”

Section: Purification Of Sdg Sdg Extractionmentioning

confidence: 99%

“…On this basis, Chen, Liu, Shi, and Ma (2007) developed a high performance liquid chromatography (HPLC) method with ultraviolet (UV) detection for the analysis of SDG in flaxseed, which involves extraction from defatted flaxseed flour (DFF) with methanol and basic hydrolysis with aqueous sodium hydroxide. To simplify this extraction methodology, Eliasson, Kamal-Eldin, Andersson, and Åman (2003) eliminated the alcohol extraction step by using aqueous alkaline hydrolysis directly, resulting in a higher yield than that obtained by the hydrolysis of alcoholic extracts (Johnsson, Kamal-Eldin, Lundgren, & Åman, 2000). Other technique includes the use of supercritical CO 2 for lignan extraction from flaxseed which results in lower SDG extraction compared to the traditional solvent extractions (Comin, Temelli, & Saldaña, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Johnsson et al (2000) have purified SDG from flaxseed using chromatographic methods. Starting with an extraction with 1,4-dioxane/ethanol and alkaline hydrolysis, they were able to purify SDG by reverse-phase chromatography using silica gel, and they were finally able to confirm the identity of the compound by nuclear magnetic resonance (NMR).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of secoisolariciresinol diglucoside extraction from flaxseed (Linum usitatissimumL.) and isolation by a simple HPLC-UV method

Fuentealba

Figuerola

Estévez

et al. 2014

CyTA - Journal of Food

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Optimization of secoisolariciresinol diglucoside extraction from flaxseed (Linum usitatissimum L.) and isolation by a simple HPLC-UV method Optimización de la extracción de secoisolariciresinol diglucósido desde linaza (Linum usitatissimum L.) y su purificación a través de un método simple HPLC-UV This research was conducted to develop and optimize a methodology to quantify the content of secoisolariciresinol diglucoside (SDG), the main vegetable lignan present in flaxseed. However, at the time of this work, commercial SDG was not available for standard use. Therefore, an effective method to obtain purified SDG from flaxseed was developed using chromatographic techniques. The SDG obtained was of approximately 97% purity. The methanol extraction time, alkali concentrations, and reaction temperature were optimized through a response surface methodology (RSM), and the optimal conditions established for the extractions were 47°C, 58 mmol L −1 sodium methoxide, and 24 h. This methodology was then applied to study the variation of SDG content in flaxseed from different cultivars. The SDG content in different varieties of flaxseed ranged from 10.8 to 17.9 mg g −1 in defatted flaxseed flour and from 6.0 to 10.9 mg g −1 in whole flaxseed.Keywords: flaxseed; lignan; secoisolariciresinol diglucoside; RSM El objetivo de esta investigación fue desarrollar y optimizar una metodología para cuantificar el contenido de secoisolariciresinol diglucósido (SDG), el cual es el principal lignano vegetal presente en linaza. Sin embargo, al realizar este trabajo no existía SDG comercial para ser utilizado como estándar externo. Por lo tanto, se desarrolló un método efectivo para obtener SDG purificado usando técnicas cromatográficas. El SDG obtenido fue de 97% de pureza. Se optimizó el tiempo de extracción metanólica, concentración de alcali y temperatura de reacción a través de un diseño de superficie respuesta. Las condiciones óptimas establecidas fueron: 47°C, 58 mmol L −1 de metóxido de sodio y 24 h. Esta metodología luego fue aplicada para estudiar la variación del contenido de SDG en linaza de diferentes cultivares. El contenido de SDG en diferentes variedades de linaza se encontró en el rango de 10,8 a 17,9 mg g −1 en harina desgrasada de linaza y de 6,0 a 10,9 mg g −1 en linaza entera.

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Secondary Metabolites in Fruits, Vegetables, Beverages and Other Plant‐based Dietary Components

Crozier¹,

Yokota²,

Jaganath³

et al. 2006

Plant Secondary Metabolites

111

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HPLC Method for Analysis of Secoisolariciresinol Diglucoside in Flaxseeds

Cited by 191 publications

References 15 publications

Review: Feed intake, milk production and milk composition of dairy cows fed flaxseed

Review: Feed intake, milk production and milk composition of dairy cows fed flaxseed

Optimization of secoisolariciresinol diglucoside extraction from flaxseed (Linum usitatissimumL.) and isolation by a simple HPLC-UV method

Secondary Metabolites in Fruits, Vegetables, Beverages and Other Plant‐based Dietary Components

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