HPLC-ESI-MSn Analysis and Validation of UV-Vis and RP-HPLC-PDA Methods for Polyphenols Quantification from Hymenaea eriogyne

Tenório, Camylla Janiele Lucas; Silva, Sarah Luanne; Silva, Joanda Paolla Raimundo e; Tavares, Josean Fechine; Ferreira, Magda Rhayanny Assunção; Soares, Luiz Alberto Lira

doi:10.1007/s43450-022-00260-y

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…The recovery of bioactive compounds is directly linked to the extraction process, which can be influenced by the characteristics of the sample, the extraction technique, and the extracting solvent [27] . As for the solvent, the use of two or more solvents with different polarities, instead of a single solvent, can increase the extraction of phytochemical compounds [28,29] . Among the solvents used in the extraction of polyphenols, such as flavonoids and hydroxycinnamic derivatives, the mixture of acetone and water has been widely employed, and its use has been reported in the extraction of bioactive compounds from A. hispidum [8,30,31] .…”

Section: Resultsmentioning

confidence: 99%

“…[27] As for the solvent, the use of two or more solvents with different polarities, instead of a single solvent, can increase the extraction of phytochemical compounds. [28,29] Among the solvents used in the extraction of polyphenols, such as flavonoids and hydroxycinnamic derivatives, the mixture of acetone and water has been widely employed, and its use has been reported in the extraction of bioactive compounds from A. hispidum. [8,30,31] In this work, the acetone: water mixture was effective in extracting the phenolic compounds from the A. hispidum matrix, as evidenced in the chromatographic analyses.…”

Section: Characterization Of Crude Extract and Fractionsmentioning

confidence: 99%

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Evaluation of the Antioxidant and Antifungal Actions of the Optimized Crude Extract and Fractions from the Aerial Parts of Acanthospermum hispidum

Santos

Silva

Machado

et al. 2022

Chemistry & Biodiversity

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This study investigated the phytochemical characteristics of the aerial parts of Acanthospermum hispidum, by chromatographic and spectrophotometric methods, and evaluated the antioxidant and antifungal activities of the crude extract and polyphenol‐enriched fractions of the species. The phytochemical prospection showed the presence of polyphenols from the groups of hydroxycinnamic derivatives and flavonoids in the crude extract (CE) and fractions of the aerial parts of A. hispidum. In the chromatographic analysis, it was possible to observe that the fractionation process of the CE with hexane and ethyl acetate was efficient in enriching the fractions in phenolic compounds. This enrichment provided an increase in antioxidant activity by the DPPH and ABTS methods, in which it was observed a higher antioxidant activity for EAF in the DPPH test and higher activity against the ABTS radical by the fractions AqF and RAqF. The extract and fractions were effective against Candida non‐Candida albicans strains, mainly against C. glabrata, C. parapsilosis and C. krusei, acting predominantly fungicidal. The results indicate that the aerial parts of A. hispidum can serve as a basis for the development of new antioxidant and antifungal products. Moreover, the fractionation process can contribute to increasing the biological potential of the species.

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Section: Resultsmentioning

confidence: 99%

Section: Characterization Of Crude Extract and Fractionsmentioning

confidence: 99%

Evaluation of the Antioxidant and Antifungal Actions of the Optimized Crude Extract and Fractions from the Aerial Parts of Acanthospermum hispidum

Santos

Silva

Machado

et al. 2022

Chemistry & Biodiversity

Self Cite

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“…Among the various analytical techniques, high-performance liquid chromatography (HPLC) coupled with a diode array detector (DAD) stands out for its simplicity and effectiveness, making it the most popular technique for qualitatively and quantitatively investigating natural polyphenols in various botanical products [20]. Numerous analytical methods have been reported for analyzing complex plant extracts using HPLC combined with different detection systems, including UPLC-DAD [21], HPLC-DAD-MS [22], HPLC-ELSD [23], HPLC-PDA [24], HPLC-PDA-MS [25], and LC-MS [26].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Pharmacologically Important Polyphenolic Secondary Metabolites in Plant-based Food Samples Using HPLC-DAD

Akhtar,

Siddiqui,

Ramzan

et al. 2024

Plants

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Polyphenolic compounds are vital components of plants. However, their analysis is particularly difficult and challenging due to their similar chemical and structural properties. In this study, we developed a simple and reproducible HPLC-DAD protocol for determining nineteen pharmacologically important polyphenols in plant-based food samples, including fruits (apple, banana, grapefruit, peach, grapes, plum, and pear), vegetables (onion, cabbage, capsicum, garlic, lemon, tomato, potato, and spinach), and other edible items (corn, kidney beans, green tea, black tea, and turmeric). The reference standards were pooled into four different groups based on logP values and expected retention time to avoid compound co-elution. These developed methods will be useful for the qualitative and quantitative analysis of biologically important polyphenolic compounds in various food samples and botanicals.

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Phenolic Compounds of Fresh and Dried Figs: Characterization and Health Benefits

Debib¹,

Menadi²

2023

Fig (Ficus Carica): Production, Processing, and Properties

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HPLC-ESI-MSn Analysis and Validation of UV-Vis and RP-HPLC-PDA Methods for Polyphenols Quantification from Hymenaea eriogyne

Cited by 5 publications

References 27 publications

Evaluation of the Antioxidant and Antifungal Actions of the Optimized Crude Extract and Fractions from the Aerial Parts of Acanthospermum hispidum

Evaluation of the Antioxidant and Antifungal Actions of the Optimized Crude Extract and Fractions from the Aerial Parts of Acanthospermum hispidum

Investigation of Pharmacologically Important Polyphenolic Secondary Metabolites in Plant-based Food Samples Using HPLC-DAD

Phenolic Compounds of Fresh and Dried Figs: Characterization and Health Benefits

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