HPLC Determination of Vitamin E in Fortified Foods

Abstract

Vitamin E is a generic name for tocol derivatives having the biological function of α -tocopherol. Four tocopherols and four tocotrienols (Figure 16.1 ) are generally reported, although other tocol derivatives with limited distribution have also been found in plant sources [1] .The most common natural E vitamers in the diet are α -and γ -tocopherols, which are available from green leaves and vegetables ( α -tocopherol) and from oilseeds, vegetable oils, and nuts (both α -and γ -tocopherols). Tocotrienols ( α -, β -and γ -) are found in cereal grains and palm oil. The main recognized biochemical effects of the tocopherols relate to their ability to act as antioxidants and to protect the polyunsaturated fatty acids in foods and in cell membranes and other compartments from oxidative damage caused by over -production of free radicals [2, 3] . Other physiological effects of vitamin E such as inhibition of protein kinase -C or smooth muscle cell proliferation [4] might or might not relate to its antioxidant function [3] . Food fortifi cation with vitamin E is performed for two main reasons:• as a vitamin; to meet the vitamin E requirements of the population • as an antioxidant; to protect unstable polyunsaturated fatty acids in certain foods against oxidation by free radicals.The purpose of this chapter is to describe the foods used for vitamin E fortifi cation, the chemical nature and levels of fortifi cants, and the methods used for their analysis. Fortifi cation of Foods with Vitamin EDietary reference intake s ( DRI s) are used to specify reference values used for planning and assessing nutrient intake for healthy people based on α -tocopherol.

Effects of dietary protected fat and vitamin E on in vitro rumen fermentation characteristics and reproductive performances of Hanwoo cows

“…From the collected blood plasma, the metabolite parameters, including vitamin E, blood urea nitrogen (BUN), and glucose, and the hormone parameters, including LH, FSH, progesterone, and estrogen, were measured. The vitamin E concentration in the plasma was determined using an HPLC-MS (SCIEX API 5000 TM AB Sciex Pte, Ltd., Woodlands, Singapore) fitted with a UV detector (SecurityGuard TM ULTRA EVO C18, Phenomenex Inc., California, USA) and a column (Kinetex 5μm EVO C18 100x2.1 mm, Phenomenex Inc., California, USA) according to [20]. The plasma concentration of the blood urea nitrogen was determined using a UREA/BUN kit (Roche, Mannheim, Germany).…”

Review: - Determination of vitamin E concentration in different samples

“…Derivatives of vitamin E (tocopherols) can be extensively analyzed using high-performance liquid chromatography (HPLC) method that utilizes natural and reverse-phase columns by using various detectors such as UV-Vis, fluorescence, electrochemical, and mass spectrometric. The preference of the analytical method depends on the nature of the forficate and the sample matrix, as Afaf and Jelena mentioned [6]. Korchazhkina and his colleagues [7] elucidated that the removal of vitamin E obtained from human milk using hexane with and without preform has been compared.…”