“…[2][3][4] Also, an efficient determination of RF concentration is significative in forages, food, supplements, and quality control. Thus, number of strategies including High Pressure-Liquid Chromatography (HPLC), 5 Chromatographic methods, 6 Colorimetric methods, 7 and Optical methods 8 have been used for the detection of RF. But, wide range of pre-treatment steps, prolonged analysis times, poor sensitivity and expensive nature of these methods limit their precedence in the laboratory condition.…”