HPLC Determination of Sildenafil in Tablets

Abstract

Objective: The popularity of Sildenafil, the widespread distribution of various products and dietary supplements with added synthetic drugs, requires reliable analysis methods. This research study aimed to develop a simple isocratic HPLC method for the determination of Sildenafil in tablet dosage forms from the local market.
 Methods: Separation was carried out at 30 °C, using column LiChrosorb® RP-18 (150 x 4.0 mm, 5 μm) with mobile phase consisting of acetonitrile: methanol: 0.5% triethylamine (15: 26: 59 v/v/v). The detector was set at 290 nm. The flow rate was 1.0 ml/min and the injection volume was 20 μl.
 Results: Linear correlation was obtained within the range 6.25–50.0 μg/ml with correlation coefficient (R2) 0.9998. The achieved limits of detection and quantitation were 0.7 and 2.2 μg/ml, respectively.
 Conclusion: The developed method can be applied for the quality control of Sildenafil preparations.