HPLC determination of irbesartan in human plasma: its application to pharmacokinetic studies

Abstract

A simple and rapid HPLC method using fluorescence detection was developed for determination of irbesartan in human plasma. Sample preparation was accomplished through a simple deproteinization procedure with 0.4 mL of acetonitrile containing 800 ng/mL of losartan (internal standard), and to a 0.1 mL plasma sample. Chromatographic separation was performed on a Zorbax Xclipse XDB C18 column (150 x 4.6 mm, i.d., 5 microm) at 40 degrees C. An isocratic mobile phase, acetonitrile:0.1% formic acid (37:63, v/v), was run at a flow-rate of 1.0 mL/min, and the column eluent was monitored using a fluorescence detector set at excitation and emission wavelengths of 250 and 370 nm, respectively. The retention times of irbesartan and losartan were 4.4 and 5.9 min, respectively. This assay was linear over a concentration range of 10-5000 ng/mL with a lower limit of quantification of 10 ng/mL. The coefficient of variation for this assay precision was less than 8.48%, and the accuracy exceeded 94.4%. The mean relative recoveries of irbesartan and losartan were 98.4 and 99.1%, respectively. This method was successfully applied for pharmacokinetic study after oral administration of irbesartan (300 mg) to 23 Korean healthy male volunteers.

Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase Regulation in Human Carotid Atheroma <i>in vitro </i>

“…The concentration of interventional agents used were based on circulating concentrations measured in patients receiving therapeutic levels of the drug or published effective in vitro concentrations. Therapeutic circulating concentrations of irbesartan are reported to be between ~1-10 mmol/L 25,37) . Pharmacokinetic studies suggest that the effective inhibitory dose of PD123319 for use in in vitro culture is 0.1-1 µmol/L 38) .…”

Quantitation of irbesartan and major proteins in human plasma by mass spectrometry with time-of-flight analyzer

“…LC is the major method for measurement of irbesartan in human plasma and urine. It is combined with ultraviolet (UV) detector [4,5], diode array detector (DAD) [6][7][8], fluorescence (Flu) detector [9][10][11][12][13], electrospray ionization mass spectrometric detection [14], and tandem quadrupole mass spectrometer [15]. In the CE technique, all the relative references apply in pharmaceutical formulation, except Zhang et al used polymer monolith microextraction as a clean-up procedure and capillary zone electrophoresis to analyze irbesartan and three other sartans in urine [16].…”

“…Several analytical methods have been developed for the determination of irbesartan including liquid chromatography (LC) [4][5][6][7][8][9][10][11][12][13][14][15], capillary electrophoresis (CE) [16][17][18][19] and spectrophotometry [20][21][22]. LC is the major method for measurement of irbesartan in human plasma and urine.…”

Simultaneous Determination of Sartans by High Performance Liquid Chromatography with Ultra Violet Detection

“…Irbesartan in a biological material: plasma and urine [31][32][33][34][35] is determined by an HPLC analysis with C18 column and DAD 33,34 , MS detector 35 or fluorescence detector, 31 Irbesartan and hydrochlorothiazide in tablets are analyzed spectrophotometrically. …”