“…A number of derivatizing reagents have been proposed for HPLC determination of guanidino compounds, mainly benzoin, [12][13][14] ninhydrin, 15,16 9,10-phenanthrenequinone, 17 anisoin, 18 furoin 19 and pyridoin. 20 Gas chromatography has high potential for the separation of organic compounds with low running cost, and analysis could be completed with short running time. The GC of guanidino compounds has been carried out aer precolumn derivatization with triuoroacetic anhydride, 21 hexauoroacetylacetone, 22 hexa-uoroacetylacetone mono (trimethylsilyl) triuoroacetamide, 23,24 hexauoroacetylacetone pentauorobenzyl bromide, 25 hexa-uoroacetylacetone and ethyl chloroformate.…”