HPLC Determination of Flavonoids in Hairy-Root Cultures of Scutellaria baicalensis Georgi

Kovács, Gy. Huber; Кузовкина, И. Н.; Szöke, Éva; Kursinszki, L.

doi:10.1365/s10337-004-0266-0

Cited by 44 publications

(21 citation statements)

References 11 publications

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“…Therefore, from their data, it is impossible to judge about the ratios between particular flavones. In similar experiment, we demonstrated clearly that an increased sucrose concentration in the nutrient medium induced a considerable accumulation in skullcap roots of only glucuronides but not aglycons [19].…”

Section: Discussionsupporting

confidence: 65%

Flavones in genetically transformed Scutellaria baicalensis roots and induction of their synthesis by elicitation with methyl jasmonate

Кузовкина

Guseva

Szöke³

et al. 2005

Russ J Plant Physiol

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The composition and content of flavones were estimated in pRi T-DNA-transformed skullcap ( Scutellaria baicalensis Georgi) roots obtained by the inoculation of axenically grown seedlings with a wild A4 strain of the soil bacterium Agrobacterium rhizogenes. It was elucidated by analytical and preparative HPLC of phenolic compounds in the extracts from the pRi T-DNA-transformed roots and also by ultraviolet spectroscopy and 1 H and 13 C NMR that cultured skullcap roots contained similar basic flavones as intact roots of this plant species, i.e., baicalein and wogonin and corresponding glucuronides, baicalin and wogonoside. The content of these flavones in cultured roots was threefold lower than in the roots of intact five-year-old plants. When skullcap roots were cultured on B5 or Murashige and Skoog medium, the ratios between major flavones changed but their total content remained unchanged. The treatment of three-week-old cultured roots with methyl ether of jasmonic acid (MeJa) doubled the total concentration of major flavones in roots; the content of aglycons, baicalein and wogonin, increased to a greater degree, e.g., by 2.3 and 3.3 times, respectively. The induction of flavone production by elicitors indicates that flavones behave as phytoanticipins because major flavones of skullcap manifest a distinct antimicrobial activity. The results of the short-term treatment of skullcap roots with MeJa show that stress biotic factors can considerably increase the content of physiologically active flavones.

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Section: Discussionsupporting

confidence: 65%

Flavones in genetically transformed Scutellaria baicalensis roots and induction of their synthesis by elicitation with methyl jasmonate

Кузовкина

Guseva

Szöke³

et al. 2005

Russ J Plant Physiol

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“…Peaks were identified by addition of standards and by diode-array detection from 220 to 380 nm. The retention time of corresponding compounds referred by Kovacs et al (2004). Typical HPLC chromatogram of the flowers extract is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Secondary metabolites in Hypericum perfoliatum: variation among plant parts and phenological stages

Çırak

Radušienė²,

Janulis³

et al. 2007

Bot. Helv.

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Ç ırak C., Radušienė J., Janulis V. and Ivanauskas L. 2007. Secondary metabolites in Hypericum perfoliatum: variation among plant parts and phenological stages. Bot. Helv. 117: 29 -36.The genus Hypericum is a source of biologically active compounds, notably hypericin and various phenolics. The concentrations of these compounds in plant tissues vary among plant parts and during the growing season. To determine this variation for Hypericum perfoliatum, a widespread species in Northern Turkey, wildgrowing plants were harvested at five phenological stages: vegetative, floral budding, full flowering, fresh fruiting and mature fruiting. Whole shoots as well as individual parts (stems, leaves and reproductive tissues) were assayed for bioactive compounds by HPLC. In whole shoots, concentrations of secondary metabolites increased during shoot development. The highest concentrations of chlorogenic acid, hyperoside, quercitrin, quercetin and hypericin were reached when plants flowered, whereas rutin and apigenin-7-O-glucoside reached highest levels during fruit development. Of the individual plant parts, leaves did not contain any rutin, apigenin-7-O-glucoside and quercetin, and stems contained no hypericin. Reproductive parts had the highest levels of rutin, apigenin-7-O-glucoside, quercitrin, quercetin and hypericin, whereas leaves produced more chlorogenic acid and hyperoside. Compared to H. perforatum, a widely used medicinal plant, the tissues of H. perfoliatum contained similar concentrations of hypericin, less rutin, quercitrin and quercetin, and more chlorogenic acid and hyperoside. The presence of apigenin-7-O-glucoside had not been reported previously in the genus Hypericum.

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“…The column temperature was at 20°C. The elution was monitored at 360 nm and obtained data were compared with authentic samples of the respective compounds (Kovacs et al 2004).…”

Section: Hplc Analysismentioning

confidence: 99%

Variation of bioactive secondary metabolites in Hypericum origanifolium during its phenological cycle

Çırak

Radušienė²,

Ivanauskas³

et al. 2007

Acta Physiol Plant

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The genus Hypericum has received considerable interest from scientists, as it contains the variety of structurally diverse natural products which possess a wide array of biological properties. The present study was conducted to determine ontogenetic and morphogenetic variation of hypericin, chlorogenic acid and flavonoids, as rutin, hyperoside, apigenin-7-Oglucoside, quercitrin and quercetin content in Hypericum origanifolium growing in Turkey. Wild growing plants were harvested at vegetative, floral budding, full flowering, fresh fruiting and mature fruiting stages and dissected into stem, leaf and reproductive tissues and assayed for bioactive compounds by HPLC method. Hypericin, quercetin and quercitrin content in whole plant increased during course of ontogenesis and the highest level was reached in blooming stage. On the contrary, hyperoside content of whole plant decreased linearly with advancing of development stages and the highest level was observed at vegetative stage. Plants produced similar amount of chlorogenic acid at all stages of plant phenology except for mature fruiting at which the amount of this compound was decreased sharply. Among different tissues, reproductive parts accumulated the highest level of hypericin, quercetin and quercitrin, however, leaves produced substantially higher amount of chlorogenic acid and hyperoside. Rutin and apigenin-7-O-glucoside were detectable in all tissues only during fruit maturation. The presence and variation of these bioactive substances in H. origanifolium were reported for the first time.

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HPLC Determination of Flavonoids in Hairy-Root Cultures of Scutellaria baicalensis Georgi

Cited by 44 publications

References 11 publications

Flavones in genetically transformed Scutellaria baicalensis roots and induction of their synthesis by elicitation with methyl jasmonate

Flavones in genetically transformed Scutellaria baicalensis roots and induction of their synthesis by elicitation with methyl jasmonate

Secondary metabolites in Hypericum perfoliatum: variation among plant parts and phenological stages

Variation of bioactive secondary metabolites in Hypericum origanifolium during its phenological cycle

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